Staphylococcus Infection Associated with Arthroplasty

Ye, Weisheng; Shang, Wei; Yang, Yaqiong

doi:10.5772/25783

Cited by 3 publications

(3 citation statements)

References 77 publications

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“…11) B. subtilis strain WB700, lacking seven extracellular proteases (AprE, Epr, NprB, NprE, Mpr, Bpr, and Vpr), has been isolated in the background 168 strain. 12) Although the levels of most extracellular proteins were not affected in the WB700 strain, the levels of some proteins, the WapA-and YvcE-processing form, increased. 13) This result suggests that released forms of the cell wall-associated WapA and YvcE were degraded by extracellular proteases.…”

mentioning

confidence: 90%

Alkaline Serine Protease AprE Plays an Essential Role in Poly-γ-glutamate Production during Natto Fermentation

Kada

Ishikawa

Ohshima

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Natto is a traditional Japanese food made from soybeans fermented by natto starter strains of Bacillus subtilis natto. It has been suggested that extracellular protease activity released by the bacteria are involved in the production of poly--glutamate ( -PGA) during natto fermentation. One of the natto starters, strain r22, possesses at least seven genes, each of which encoded an extracellular protease orthologous to its counterpart in B. subtilis 168, aprE, bpr, epr, mpr, nprE, vpr, and wprA, but it was found to lack nprB. Inactivating the aprE ortholog alone resulted in a severe decrease in -PGA production and in the total extracellular protease activity. The defect in -PGA production of the mutant lacking the aprE ortholog was complemented when the medium was supplemented with sufficient glutamate. These results suggest that the alkaline serine protease encoded by aprE plays an indispensable role in supplying materials to produce -PGA. On the other hand, simultaneous inactivation of all the protease genes except for aprE did not significantly affect either -PGA production or total protease activity.

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confidence: 90%

Alkaline Serine Protease AprE Plays an Essential Role in Poly-γ-glutamate Production during Natto Fermentation

Kada

Ishikawa

Ohshima

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…The arthroplasty associated infection may distinguishes two major types-septic arthritis and osteomyelitis, which both cause serious morbidity and are often difficult to manage. Septic arthritis is a joint disease characterized by bacterial colonization and rapid articular destruction [1].…”

Section: Introductionmentioning

confidence: 99%

“…Osteomyelitis describes a range of infections in which the bone is colonized by microorganisms, with associated inflammation and bone destruction. Acute osteomyelitic foci are characterized by pus-forming inflammation at the site of microbial colonization [1].…”

Section: Introductionmentioning

confidence: 99%

Η Κλινική Εφαρμογή Των Μοριακών Τεχνικών Στη Διάγνωση Της Ορθοπαιδικής Λοίμωξης

Zegaer¹,

Ζιγκαέρ²

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Intraoperative conventional bacteriological cultures were compared with different polymerasechain reaction (PCR) methods in patients with total joint arthroplasties. The isolated bacteria wereinvestigated for biofilm formation, and the biofilm forming strains, in their planktonic and biofilmforms, were further tested for their antimicrobial resistance against several clinically importantantimicrobials. Forty four bone and joint samples were included and classified as infected or noninfectedaccording to standard criteria for periprosthetic hip and knee infections. For thebacteriological diagnosis, conventional culture, two types of universal PCR and species specificPCR for three selected pathogens (S. aureus, S. epidermidis, and P. aeruginosa) were applied.Biofilm formation determination was performed by the tissue culture plate method. Antimicrobialsusceptibility of the planktonic bacteria was performed by the minimal inhibitory concentrationdetermination and, of the biofilm forms, by the minimal inhibitory concentration for bacterial regrowthfrom the biofilm. Twenty samples were culture positive, with S. epidermidis, S. aureus orP. aeruginosa. All PCR methods were very ineffective in detecting only one pathogen. All isolateswere biofilm positive and their biofilm forms were highly resistant. In this study, compared toPCR, culture remains the “gold standard”. The biofilm formation by the causative bacteria and theconcomitant manifold increased antimicrobial resistance may explain the clinical failure oftreatment in some cases and should be considered in the future for therapeutic planning.

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Non-contact Tomographic Imaging and Nanocomposite Thin Films for Monitoring Human-prosthesis Interfaces

Gupta

Loh

2017

Procedia Engineering

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Staphylococcus Infection Associated with Arthroplasty

Cited by 3 publications

References 77 publications

Alkaline Serine Protease AprE Plays an Essential Role in Poly-γ-glutamate Production during Natto Fermentation

Alkaline Serine Protease AprE Plays an Essential Role in Poly-γ-glutamate Production during Natto Fermentation

Η Κλινική Εφαρμογή Των Μοριακών Τεχνικών Στη Διάγνωση Της Ορθοπαιδικής Λοίμωξης

Non-contact Tomographic Imaging and Nanocomposite Thin Films for Monitoring Human-prosthesis Interfaces

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