Staphylococcus pasteuri (BCVME2) Resident in Buffalo Cervical Vaginal Mucus: A Potential Source of Estrus-Specific Sex Pheromone(s)

Srinivasan, Mahalingam; Rengarajan, Rengasamy Lakshminarayanan; Dhanasekaran, D.; Akbarsha, Mohammad Abdulkader; Archunan, Govindaraju

doi:10.1007/s10886-021-01311-9

Cited by 2 publications

(1 citation statement)

References 45 publications

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“…According to Ziegler et al [10], the ovulation timing and sexual behavior coordination are considered to be essential factors for successful fertilization [12]. A number of scientists have demonstrated that females release specific odour(s) during estrus through urine [13][14][15][16], feces [17], and vaginal fluid [18] which constitute the major avenues for chemical communication in mammals [19] and may be considered an accurate indicators of female receptivity [9,20].…”

Section: Introductionmentioning

confidence: 99%

Identification of urinary sex pheromones in female Rajapalayam breed dog and their influence on stud male reproductive behaviour

Rajagopal¹,

Dhivya²,

Ponmanickam³

et al. 2022

J App Biol Biotech

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The dog has been a good companion animal to humans. Companionship, security, hunting, and herding are their best utilities for the owner. The amazing sense of smell of some of the breeds of dogs is taken to advantage to train for accurately tracking and sniffing out criminals. The heralding and tracking efficiencies of the majestic Rajapalayam breed of dog are outstanding but little is documented about the reproductive status of females through volatile compounds to which the males respond appropriately to facilitate sexual attraction and mating of this breed. Therefore, the present study was carried out to investigate the reproductive behaviors and urinary volatiles at various stages of the estrous cycle. The repeated behaviors such as approach, attempted investigation, investigation, premount contact, and mounting were significantly higher in male dogs exposed to females in estrus. By contrast, such reproductive behaviors were totally absent in males exposed to females in post-estrus. Pre-copulatory behaviors and urinary volatiles of female dogs were hypothesized to be the guiding/exhibitioner factors. As many as, 30 urinary volatile compounds were identified during the estrous cycle. Among these, 2-methyl-hexadecane, 3-methylene-4methypentan-1-ol, (E)-9-methyl-7-undecenoic acid, 4,5-dimethyl-4-hexen-2-one, and 2-octyldodecan-1-ol were specific to estrus phase. Behavioral observation clearly revealed that stud males exhibited the highest attraction to females, with repeated attempts of investigation, actual investigation, mounting, and penile erection being significantly high toward estrus urine sample as well as mixture of synthetic estrus-specific volatile compounds. However, proestrus-specific 2, 3-dimethyl-1-butanol and postestrus-specific pentadecane did not evoke such sexual behaviors in male dogs. It is concluded that the female dog in estrus phase releases specific volatiles chemicals in the urine, which is instrumental in provoking the manifestations of premating behaviors in male dogs. Thus, the present study provides evidence that non-invasive methods such as assessment of behaviors and estrus-specific urinary volatiles would serve as good for detection of estrus in the Rajapalayam dog. Among the volatiles identified in estrus urine, based on the behavioral assay, urinary (E)-9-methyl-7-undecenoic acid proves to be the most effective male-attracting pheromone in the female dog. Thus, the present study provides evidence to the effect that assessment of male behaviors to estrusspecific urinary volatiles can facilitate detection of estrus in the Rajapalayam dog so as to precisely time the mating.

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Section: Introductionmentioning

confidence: 99%

Identification of urinary sex pheromones in female Rajapalayam breed dog and their influence on stud male reproductive behaviour

Rajagopal¹,

Dhivya²,

Ponmanickam³

et al. 2022

J App Biol Biotech

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The Exoproteome of Staphylococcus pasteuri Isolated from Cervical Mucus during the Estrus Phase in Water Buffalo (Bubalus bubalis)

et al. 2022

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Bacterial extracellular proteins participate in the host cell communication by virtue of the modulation of pathogenicity, commensalism and mutualism. Studies on the microbiome of cervical mucus of the water buffalo (Bubalus bubalis) have shown the occurrence of Staphylococcuspasteuri and that the presence of this bacterium is indicative of various physiological and reproductive states in the host. Recently, S. pasteuri has been isolated from the cervical mucus of the buffalo during the different phases of estrous cycle, and has proved to be much more pronounced during the estrus phase. The basis underlying the availability of a significantly increased S. pasteuri population, specifically during the estrus phase, is not known. Consequently, it is important to determine the significance of the specific abundance of S. pasteuri during the estrus phase of the buffalo host, particularly from the perspective of whether this bacterial species is capable of contributing to sexual communication via its extracellular proteins and volatiles. Therefore, the relevance of S. pasteuri exoproteome in the buffalo cervical mucus during the estrus phase was analyzed using LC-MS/MS. As many as 219 proteins were identified, among which elongation factor Tu (EF-Tu), 60-kDa chaperonin (Cpn60), enolase, fructose-bisphosphate aldolase class 1 (FBP aldolase), enoyl-[acyl-carrier-protein] reductase [NADPH] (ENR) and lipoprotein (Lpp) were the functionally important candidates. Most of the proteins present in the exoproteome of S. pasteuri were those involved in cellular–metabolic functions, as well as catalytic- and binding activities. Moreover, computational studies of Lpp have shown enhanced interaction with volatiles such as acetic-, butanoic-, isovaleric- and valeric acids, which were identified in the cervical mucus S. pasteuri culture supernatant. The present findings suggest that S. pasteuri extracellular proteins may play an important role in buffalo sexual communication during the estrus phase.

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Staphylococcus pasteuri (BCVME2) Resident in Buffalo Cervical Vaginal Mucus: A Potential Source of Estrus-Specific Sex Pheromone(s)

Cited by 2 publications

References 45 publications

Identification of urinary sex pheromones in female Rajapalayam breed dog and their influence on stud male reproductive behaviour

Identification of urinary sex pheromones in female Rajapalayam breed dog and their influence on stud male reproductive behaviour

The Exoproteome of Staphylococcus pasteuri Isolated from Cervical Mucus during the Estrus Phase in Water Buffalo (Bubalus bubalis)

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