Staphylococcus ratti sp. nov. Isolated from a Lab Rat

Kovařovic, Vojtěch; Sedláček, Ivo; Petřáš, Petr; Králová, Stanislava; Mašlaňová, Ivana; Švec, Pavel; Neumann‐Schaal, Meina; Botka, Tibor; Gelbíčová, Tereza; Staňková, Eva; Doškař, Jiřı́; Pantůček, Roman

doi:10.3390/pathogens11010051

Cited by 7 publications

(1 citation statement)

References 86 publications

(93 reference statements)

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“…The strains grew well in a basic set of staphylococcal media at a temperature of 30–37°C. The morphological, biochemical, and physiological characterization was performed as previously mentioned ( 7 , 60 – 62 ). Antimicrobial susceptibility testing by disc diffusion method on Mueller-Hinton agar with adherence to EUCAST guidelines ( 63 ) was performed as described previously ( 60 ).…”

Section: Methodsmentioning

confidence: 99%

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex

Kovařovic,

Finstrlová,

Sedláček

et al. 2023

Microbiol Spectr

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Novel species of coagulase-negative staphylococci, which could serve as reservoirs of virulence and antimicrobial resistance factors for opportunistic pathogens from the genus Staphylococcus , are recognized in human and animal specimens due to advances in diagnostic techniques. Here, we used whole-genome sequencing, extensive biotyping, MALDI-TOF mass spectrometry, and chemotaxonomy to characterize five coagulase-negative strains from the Staphylococcus haemolyticus phylogenetic clade obtained from human ear swabs, wounds, and bile. Based on the results of polyphasic taxonomy, we propose the species Staphylococcus brunensis sp. nov. (type strain NRL/St 16/872 T = CCM 9024 T = LMG 31872 T = DSM 111349 T ). The genomic analysis revealed numerous variable genomic elements, including staphylococcal cassette chromosome (SCC), prophages, plasmids, and a unique 18.8 kb-long genomic island SbCI ccrDE integrated into the ribosomal protein L7 serine acetyltransferase gene rimL . SbCI ccrDE has a cassette chromosome recombinase ( ccr ) gene complex with a typical structure found in SCCs. Based on nucleotide and amino acid identity to other known ccr genes and the distinct integration site that differs from the canonical methyltransferase gene rlmH exploited by SCCs, we classified the ccr genes as novel variants, ccrDE . The comparative genomic analysis of SbCI ccrDE with related islands shows that they can accumulate virulence and antimicrobial resistance factors creating novel resistance elements, which reflects the evolution of SCC. The spread of these resistance islands into established pathogens such as Staphylococcus aureus would pose a great threat to the healthcare system. IMPORTANCE The coagulase-negative staphylococci are important opportunistic human pathogens, which cause bloodstream and foreign body infections, mainly in immunocompromised patients. The mobile elements, primarily the staphylococcal cassette chromosome mec , which confers resistance to methicillin, are the key to the successful dissemination of staphylococci into healthcare and community settings. Here, we present a novel species of the Staphylococcus genus isolated from human clinical material. The detailed analysis of its genome revealed a previously undescribed genomic island, which is closely related to the staphylococcal cassette chromosome and has the potential to accumulate and spread virulence and resistance determinants. The island harbors a set of conserved genes required for its mobilization, which we recognized as novel cassette chromosome recombinase genes ccrDE . Similar islands were revealed not only in the genomes of coagulase-negative staphylococci but also in S. aureus . The comparative genomic study contributes substantially to the understanding of the evolution and pathogenesis of staphylococci.

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Section: Methodsmentioning

confidence: 99%

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex

Kovařovic,

Finstrlová,

Sedláček

et al. 2023

Microbiol Spectr

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Valid publication of new names and new combinations effectively published outside the IJSEM

Oren

2022

International Journal of Systematic and Evolutionary Microbiology

331

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Update on novel validly published taxa of bacteria isolated from domestic animals described in 2022

Lawhon,

Burbick,

Munson

et al. 2023

J Clin Microbiol

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Expansion of our knowledge of the microbial world continues to progress at a rapid rate and carries with it an associated need for recognizing and understanding the implications of those changes. Here, we describe additions of novel taxa from domestic animals published in 2022 that are validly published per the International Code of Nomenclature of Prokaryotes. These included new members of Staphylococcaceae , Moraxella nasovis sp. nov. in sheep with respiratory disease, three additions to Campylobacteraceae (including one from chickens with spotty liver disease), and multiple additions of organisms from the microbiota of dogs, pigs, and especially honeybees and other important pollinators. Noteworthy additions were associated with diseases of cattle, including mastitis, endocarditis, orchitis, and endometritis. Also described in 2022 was Pseudochrobactrum algeriense sp. nov., a member of the Brucellaceae family, isolated from the mammary lymph nodes of cows.

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Staphylococcus ratti sp. nov. Isolated from a Lab Rat

Cited by 7 publications

References 86 publications

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex

Valid publication of new names and new combinations effectively published outside the IJSEM

Update on novel validly published taxa of bacteria isolated from domestic animals described in 2022

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