Staphylococcus succinus subsp. casei subsp. nov., a Dominant Isolate from a Surface Ripened Cheese

Place, Raymond B; Hiestand, Daniel; Burri, Sandra; Teuber, Michael

doi:10.1078/0723-2020-00130

Cited by 66 publications

(38 citation statements)

References 12 publications

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“…Comparing the results of the two commercial kits used, the VITEK 2 kit showed more misidentifications, mainly due to the limited database of gram-positive cocci (19 staphylococcal species) included in the ID-GBP card, whereas ID 32 Staph covers 24 Staphylococcus species. Beside the utmost importance of S. aureus identification, assured identification results, at least at the species level, are vital for differentiation of the expanding number of CoNS (sub)species increasingly characterized as emerging pathogens (22,40) and also recently recovered as food-fermenting microorganisms, which may have to be considered relevant to food safety and quality (27,28). Differentiation up to the species level may also have substantial consequences for the management of patients in the case of CoNS.…”

Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Quality-Controlled Ribosomal Sequence Database for 16S Ribosomal DNA-Based Identification of Staphylococcus Species

Becker¹,

et al. 2004

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To establish an improved ribosomal gene sequence database as part of the Ribosomal Differentiation of Microorganisms (RIDOM) project and to overcome the drawbacks of phenotypic identification systems and publicly accessible sequence databases, both strands of the 5 end of the 16S ribosomal DNA (rDNA) of 81 type and reference strains comprising all validly described staphylococcal (sub)species were sequenced. Assuming a normal distribution for pairwise distances of all unique staphylococcal sequences and choosing a reporting criterion of >98.7% similarity for a "distinct species," a statistical error probability of 1.0% was calculated. To evaluate this database, a 16S rDNA fragment (corresponding to Escherichia coli positions 54 to 510) of 55 clinical Staphylococcus isolates (including those of the small-colony variant phenotype) were sequenced and analyzed by the RIDOM approach. Of these isolates, 54 (98.2%) had a similarity score above the proposed threshold using RIDOM; 48 (87.3%) of the sequences gave a perfect match, whereas 83.6% were found by searching National Center for Biotechnology Information (NCBI) database entries. In contrast to RIDOM, which showed four ambiguities at the species level (mainly concerning Staphylococcus intermedius versus Staphylococcus delphini), the NCBI database search yielded 18 taxon-related ambiguities and showed numerous matches exhibiting redundant or unspecified entries. Comparing molecular results with those of biochemical procedures, ID 32 Staph (bioMérieux, Marcy I'Etoile, France) and VITEK 2 (bioMérieux) failed to identify 13 (23.6%) and 19 (34.5%) isolates, respectively, due to incorrect identification and/or categorization below acceptable values. In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants.The emerging role of coagulase-negative staphylococci (CoNS), along with Staphylococcus aureus, in connection with the expanding number of staphylococcal (sub)species described necessitates their comprehensive and accurate identification. A variety of methods have been proposed for the identification of staphylococcal species, including conventional identification schemes based on publications of Kloos and Schleifer (18) and commercial identification tests. In general, methods based on phenotypic characteristics are hampered by the fact that they are dependent on the expression of metabolic activities and/or morphological features. Furthermore, commercial systems often offer two or more suggestions for identification with a comparable safety level.Nowadays, with the advent of molecular biology-based techniques, investigations based on comparative DNA sequence analysis of the genes of conserved macromolecules have become commonplace in microbiology as a tool for classification of microbial organisms. The most useful and extensively investigated taxonomic marker molecules are the larger rRNAs and their corresponding ...

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Section: Discussionmentioning

confidence: 99%

Development and Evaluation of a Quality-Controlled Ribosomal Sequence Database for 16S Ribosomal DNA-Based Identification of Staphylococcus Species

Becker¹,

et al. 2004

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“…Susceptibility to novobiocin (5 mg), bacitracin (0?04 IU), furazolidone (100 mg) and polymyxin B (300 IU) was tested by the disc diffusion method on Mueller-Hinton agar (Oxoid) according to Woods & Washington (1995). Evaluation of biochemical test results was carried out using TNW 6.0 software (PLIVA-Lachema) and published phenotype characteristics described by Lambert et al (1998), Place et al (2002) and Schleifer et al (1984).…”

Section: Methodsmentioning

confidence: 99%

Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens

Nováková

Sedláček

Pantůček

et al. 2006

Journal of Medical Microbiology

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“…g 21 for smear Gruyère cheeses within the first 3 weeks of ripening. This flora is composed of two principal Gram-positive groups: coryneform bacteria (irregular rods or cocci, catalase-positive) and staphylococci (cocci, catalase-positive) (Bockelmann & Hoppe-Seyler, 2001;Bockelmann, 2002;Brenann et al, 2002;Place et al, 2002Place et al, , 2003. These two groups present similar physiological properties permitting their growth on the cheese surface: they are aerobic, alkaliphilic, mesophilic and salt-tolerant and cannot develop under acid conditions.…”

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confidence: 99%

Arthrobacter bergerei sp. nov. and Arthrobacter arilaitensis sp. nov., novel coryneform species isolated from the surfaces of cheeses

Irlinger

Bimet

Delettre

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

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Fourteen isolates of two different bacterial species isolated from the surface of smear-ripened cheeses were found to exhibit many characteristics of the genus Arthrobacter. The isolates were aerobic, Gram-positive, catalase-positive, non-spore-forming and non-motile. The cell-wall peptidoglycan contained lysine, alanine and glutamic acid. rrs sequence analysis indicated that the new isolates Re117T and Ca106T are closely related to the Arthrobacter nicotianae group and showed highest sequence similarity (>98 %) to Arthrobacter nicotianae and Arthrobacter protophormiae. However, DNA–DNA hybridization studies indicated that the strains represented two novel genomic species within the genus Arthrobacter and did not belong to A. nicotianae or A. protophormiae (<43 % DNA–DNA relatedness). On the basis of the phylogenetic and phenotypic distinctiveness of the new isolates, these bacteria should be classified as two novel Arthrobacter species, for which the names Arthrobacter bergerei sp. nov. and Arthrobacter arilaitensis sp. nov. are proposed. Type strains have been deposited in culture collections as Arthrobacter bergerei Ca106T (=CIP 108036T=DSM 16367T) and Arthrobacter arilaitensis Re117T (=CIP 108037T=DSM 16368T).

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Staphylococcus succinus subsp. casei subsp. nov., a Dominant Isolate from a Surface Ripened Cheese

Cited by 66 publications

References 12 publications

Development and Evaluation of a Quality-Controlled Ribosomal Sequence Database for 16S Ribosomal DNA-Based Identification of Staphylococcus Species

Development and Evaluation of a Quality-Controlled Ribosomal Sequence Database for 16S Ribosomal DNA-Based Identification of Staphylococcus Species

Staphylococcus equorum and Staphylococcus succinus isolated from human clinical specimens

Arthrobacter bergerei sp. nov. and Arthrobacter arilaitensis sp. nov., novel coryneform species isolated from the surfaces of cheeses

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