Staphylococcus taiwanensis sp. nov., isolated from human blood

Lin, Yu Tzu; Hung, Wei‐Chun; Wan, Tsai‐Wen; Li, Hsin; Lee, Tai-Fen; Hsueh, Po‐Ren; Teng, Lee‐Jene

doi:10.1099/ijsem.0.005262

Cited by 10 publications

(3 citation statements)

References 37 publications

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“…Multilocus sequence analysis (MLSA) based on housekeeping gene sequences provides greater discriminative power than the 16S rRNA gene for identifying and classifying the genus Staphylococcus [ 55 ]. Lin et al [ 56 ] successfully used an MLSA scheme based on six housekeeping genes ( dnaJ , gap , hsp60 , rpoB , sodA , and tuf ) to discriminate between a novel species of Staphylococcus hsinchuensis and its close relatives. Hence, these genes could act as taxonomic markers and were used to characterize the novel strain.…”

Section: Resultsmentioning

confidence: 99%

Staphylococcus hsinchuensis sp. nov., Isolated from Soymilk

Wang,

Lin,

Hsieh

et al. 2024

Pathogens

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A novel coagulase-negative Staphylococcus strain (H164T) was isolated from soymilk in Taiwan. Comparative sequence analysis of the 16S rRNA gene revealed that the H164T strain is a member of the genus Staphylococcus. We used multilocus sequence analysis (MLSA) and phylogenomic analyses to demonstrate that the novel strain was closely related to Staphylococcus gallinarum, Staphylococcus nepalensis, Staphylococcus cohnii, and Staphylococcus urealyuticus. The average nucleotide identity and digital DNA-DNA hybridization values between H164T and its closest relatives were <95% and <70%, respectively. The H164T strain could also be distinguished from its closest relatives by the fermentation of fructose, maltose, maltose, trehalose, and mannitol; the activities of α-glucosidase; and alkaline phosphatase. The major cellular fatty acids and predominant menaquinones were C15:0 iso and C15:0 anteiso and MK-7 and MK-8, respectively. In conclusion, this strain represents a novel species, named Staphylococcus hsinchuensis sp. nov., with the type strain H164T (=BCRC 81404T = NBRC 116174T).

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Section: Resultsmentioning

confidence: 99%

Staphylococcus hsinchuensis sp. nov., Isolated from Soymilk

Wang,

Lin,

Hsieh

et al. 2024

Pathogens

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“…1A ). Because the 16S rRNA analysis has limited discriminatory power in the genus Staphylococcus ( 5 , 6 , 23 ), the phylogenetic position of the new isolates was also assessed using the concatenated multilocus sequence data of six routinely used conserved housekeeping genes: rpoB , hsp60 , dnaJ , tufA , gap , and sodA for discrimination of staphylococcal species ( Fig. 1B ).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the standard microbial and molecular diagnostic tools have limited discriminatory power to reliably distinguish species closely related to S. haemolyticus ( 4 ). Only recently, molecular diagnostics approaches, mainly in-depth whole-genome characterization, have assigned atypical S. haemolyticus strains isolated from clinical specimens into the new species Staphylococcus borealis ( 5 ) and Staphylococcus taiwanensis ( 6 ). The core genome phylogeny also led to the reclassification of the S. petrasii phylogenetic complex ( 7 , 8 ), which now consists of three species —S.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex

Kovařovic,

Finstrlová,

Sedláček

et al. 2023

Microbiol Spectr

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Novel species of coagulase-negative staphylococci, which could serve as reservoirs of virulence and antimicrobial resistance factors for opportunistic pathogens from the genus Staphylococcus , are recognized in human and animal specimens due to advances in diagnostic techniques. Here, we used whole-genome sequencing, extensive biotyping, MALDI-TOF mass spectrometry, and chemotaxonomy to characterize five coagulase-negative strains from the Staphylococcus haemolyticus phylogenetic clade obtained from human ear swabs, wounds, and bile. Based on the results of polyphasic taxonomy, we propose the species Staphylococcus brunensis sp. nov. (type strain NRL/St 16/872 T = CCM 9024 T = LMG 31872 T = DSM 111349 T ). The genomic analysis revealed numerous variable genomic elements, including staphylococcal cassette chromosome (SCC), prophages, plasmids, and a unique 18.8 kb-long genomic island SbCI ccrDE integrated into the ribosomal protein L7 serine acetyltransferase gene rimL . SbCI ccrDE has a cassette chromosome recombinase ( ccr ) gene complex with a typical structure found in SCCs. Based on nucleotide and amino acid identity to other known ccr genes and the distinct integration site that differs from the canonical methyltransferase gene rlmH exploited by SCCs, we classified the ccr genes as novel variants, ccrDE . The comparative genomic analysis of SbCI ccrDE with related islands shows that they can accumulate virulence and antimicrobial resistance factors creating novel resistance elements, which reflects the evolution of SCC. The spread of these resistance islands into established pathogens such as Staphylococcus aureus would pose a great threat to the healthcare system. IMPORTANCE The coagulase-negative staphylococci are important opportunistic human pathogens, which cause bloodstream and foreign body infections, mainly in immunocompromised patients. The mobile elements, primarily the staphylococcal cassette chromosome mec , which confers resistance to methicillin, are the key to the successful dissemination of staphylococci into healthcare and community settings. Here, we present a novel species of the Staphylococcus genus isolated from human clinical material. The detailed analysis of its genome revealed a previously undescribed genomic island, which is closely related to the staphylococcal cassette chromosome and has the potential to accumulate and spread virulence and resistance determinants. The island harbors a set of conserved genes required for its mobilization, which we recognized as novel cassette chromosome recombinase genes ccrDE . Similar islands were revealed not only in the genomes of coagulase-negative staphylococci but also in S. aureus . The comparative genomic study contributes substantially to the understanding of the evolution and pathogenesis of staphylococci.

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Staphylococcus , Micrococcus , and Other Catalase‐Positive Cocci

Becker,

David,

Skov

2023

ClinMicroNow

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Historically, the genera Staphylococcus and Micrococcus were placed together with the genera Stomatococcus and Planococcus in the family Micrococcaceae , containing Gram‐positive, catalase‐positive cocci. As staphylococci and micrococci are part of the microbiota of the skin and mucous membranes, careful procedures should be used to isolate organisms from the presumed focus of infection without collecting surrounding microbiota. Because serological testing for antistaphylococcal antibodies lacks specificity and predictive accuracy, it plays no role in the diagnosis of most staphylococcal diseases. A number of studies and reviews describing the antimicrobial and biocide susceptibilities of clinically important staphylococcal species have been published. Distinguishing contaminants and colonizers from staphylococcal and micrococcal isolates causing infection continues to be an important challenge for laboratorians and clinicians.

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Staphylococcus taiwanensis sp. nov., isolated from human blood

Cited by 10 publications

References 37 publications

Staphylococcus hsinchuensis sp. nov., Isolated from Soymilk

Staphylococcus hsinchuensis sp. nov., Isolated from Soymilk

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex

Staphylococcus , Micrococcus , and Other Catalase‐Positive Cocci

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