Star-like polymer conjugates of biologically active polypeptides

Vlasov, G. P.; Illarionova, N.G.; Izvarina, N. L.; Denisov, Ilia G.

doi:10.1002/macp.1985.020091985135

Cited by 13 publications

(4 citation statements)

References 11 publications

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“…20 Before the immobilization, the disk was washed with 0.1 M PBS (pH 4.5). For immobilization of SBTI, succinylated according to the procedure described elsewhere 21 (SBTI-S), on an aminate surface of a sorbent, COOgroups of SBTI-S were activated for 30 min by water-soluble carbodiimide. The disk was then immersed into this solution and after 30 min 9 mg of SLA was added.…”

Section: Methodsmentioning

confidence: 99%

Development of a Strategy of Influenza Virus Separation Based on Pseudoaffinity Chromatography on Short Monolithic Columns

2008

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This research is devoted to the development and optimization of fine purification processes realized on short monolithic columns (CIM disks), using influenza vaccine and viruslike synthetic particles as model objects. The pseudoaffinity mode of liquid chromatography has been used as a tool for dynamic adsorption experiments. Viruslike particles, close to the dimensions of influenza viruses, were developed by means of main antigen of influenza viruses (hemeagglutinin) covalent binding to the outer aminated surface of synthetic latex particles. The natural receptor analogues of sialic acid were used as affinity ligands immobilized on the surface of the CIM disk by different ways to achieve a high adsorption capacity. Also, some other ligands were tested as possible candidates for virus capturing. The affinity binding parameters for influenza A virus were obtained by frontal elution method at optimized chromatographic conditions and immobilization schemes. The experimental data pointed out the possibility of selective isolation of hemeagglutinin from a mixture of vaccine proteins. The results obtained by fast affinity chromatography have shown functional and sterical correspondence viruslike synthetic models to influenza viruses. Additionally, the optimization of chromatographic conditions allowed isolation of influenza virus A while maintaining its virulence. The maximum value of adsorption capacity was registered for a monolithic disk, modified subsequently by chitosan and 2,6-sialyllactose and found to be equal to 6.9 x 10(12) virions/mL support.

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Section: Methodsmentioning

confidence: 99%

Development of a Strategy of Influenza Virus Separation Based on Pseudoaffinity Chromatography on Short Monolithic Columns

2008

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“…1). This may be alleviated by utilization of starlike carbon-chain polymer-protein conjugates [14][15][16] in which modifying polymer is connected with one of its termini to a single point of modified protein. that polycation protein carriers of DNA were synthesized using a protein preliminarily modified with dialdehyde with polylysine or polyethylenimine (Fig.…”

Section: Synthetic Approaches For the Preparation Of Starlike Polymermentioning

confidence: 99%

“…for insulin [14]. Similarly, the location of modification for low molecular weight proteins may be also controlled, e.g.…”

Section: The Influence Of Carbon-chain Polymer Moiety Of Protein-polymentioning

confidence: 99%

“…If any of these properties is missing, polymer-protein conjugates may not be used as BAS carriers. Biological activity (decreasing of glucose level in rabbit blood), secondary structure and immune reactivity of starlike carbon-chain polymer insulin conjugates [14] Biological properties of conjugates α-heliclity %, рН The strongest antibody response against both bradykinin and BSA was produced by those starlike polymer conjugates, in which bradykinin present in the polymethacrylic acid moiety of the conjugate was additionally modified with succinic acid. The preparation of starlike polymer-protein BSA-based conjugate based was carried out by polymerization of comonomers (acrolein diethyl acetal with methacrylic acid, acrolein diethyl acetal with N-vinylpyrrolidone and acrolein diethyl acetal and N-2-methyl-vinylimidazole) using BSA-macroinitiator prepared by modifying of BSA with 2,2'-azobisisobutyric acid dimethylimidate (Fig.…”

Section: Immunogenicity Of Starlike Polymer-protein Conjugatesmentioning

confidence: 99%

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Biodegradable and Biocompatible Carbon-Chain Polymer–Protein Conjugates as Carriers of Biologically Active Substances: Problems of Synthesis, Risks of Application and How to Overcome Them

Vlasov

2009

NATO Science for Peace and Security Series B: Physics and Biophysics

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Problems of preparation, properties and probable avenues of application of starlike carbon-chain polymer-protein conjugates as carriers of biologically active substances are presented and discussed. Special attention is directed to methodology of conjugate preparation, possible means of optimization of conjugate properties and routes of their application.Polyethylenimine or polylysine-modified proteins (transferrin and insulin) were used for DNA delivery [12,13]. The iron transporter transferrin and pancreatic hormone insulin can provide the site-specific targeting of resulting protein-polymer conjugate. At the same time, polycationic modifiers, polyethylenimine and polylysine, have been widely used for complex formation with DNA on their own. They provide the formation of a complex with DNA, which is capable of reverse ionic dissociation. It should be noted

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Synthesis, and conformational and biological study of 2‐D‐Ala,5‐des‐Met‐enkephalin hydrazide modified at the carboxylic end by poly‐N‐vinylimidazole

et al. 1987

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SynopsisN, N-bis-2-~-Ala,5-des-Met-enkephalin hydrazide of azo-isobutyric acid was synthesized. This was used to obtain polyvinylimidazole derivatives of an enkephalin analog, modified at the carboxylic end by poly-N-vinylimidazole. The effect of modification of tetrapeptide hydrazide on its conformational properties and biological activity in viw and in vitro was studied. EXPERIMENTAL Boc-LPhe-R-GPhe-Boc (I) [R=-NH-NH-CO-C(CHS),-N=N -C(CHS),-CO--NH--NH-]Boc-L-Phe-OPfp, 18.96 g (0.044 mol), was dissolved in 70 mL of dimethylformamide (DMF) and added to a suspension of hydrazide of azo-isoburyril *This paper is dedicated to the 70th anniversary of Professor G. Maneke. Soedin. A, =(lo), 2219-2228.XVII, (4), 494-499.

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Star-like polymer conjugates of biologically active polypeptides

Abstract: A general approach to the preparation of star-like carbon chain polymeric conjugates with proteins has been suggested. The influence of the modification on the spatial structure, biological activity, immunoreactivity and enzyme degradation of the protein (insulin) is discussed.

Cited by 13 publications

References 11 publications

Development of a Strategy of Influenza Virus Separation Based on Pseudoaffinity Chromatography on Short Monolithic Columns

Development of a Strategy of Influenza Virus Separation Based on Pseudoaffinity Chromatography on Short Monolithic Columns

Biodegradable and Biocompatible Carbon-Chain Polymer–Protein Conjugates as Carriers of Biologically Active Substances: Problems of Synthesis, Risks of Application and How to Overcome Them

Synthesis, and conformational and biological study of 2‐D‐Ala,5‐des‐Met‐enkephalin hydrazide modified at the carboxylic end by poly‐N‐vinylimidazole

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