Star-shaped polymers consisting of a β-cyclodextrin core and poly(amidoamine) dendron arms: binding and release studies with methotrexate and siRNA

Deng, Junjie; Li, Na; Mai, Kaijin; Yang, Chuan; Li, Yan; Zhang, Liming

doi:10.1039/c0jm03030a

Cited by 50 publications

(38 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…61 A star-shaped polymer comprising of a silsesquioxane core and 20 PDMAEMA arms with a DP of 230 was synthesised showing superior transfection efficiency even in hard-to-transfect Jurkat T-cells and primary T-lymphocytes. 63,64 As a summary it can be said that transferring the branched structure to star-shaped polymers can result in promising transfection properties while the cytotoxicity can be reduced and the transfection efficiency can be maintained or even increased. Their advantage regarding the cytotoxicity and the polyplex dissociation was demonstrated by using a POSS(SS-PDMAEMA 15-24 ) 8 star.…”

Section: Star-shaped Polymersmentioning

confidence: 99%

The influence of polymer architecture on in vitro pDNA transfection

et al. 2015

View full text Add to dashboard Cite

In 2012, the first gene therapy agent was approved by the Europe Medicines Agency leading to increased interest in this research field. Beside viruses, non-viral agents based on lipids or polymers represent aspiring alternatives to deliver the genetic material. Different hurdles have to be overcome depending on the kind of nucleic acid used, where plasmid DNA (pDNA) and small interfering RNA represent the common ones. The main challenge for transfection agents, in particular for pDNA delivery, is the transfer to the cell and into the cell nuclei. Within the group of transfection vesicles, cationic polymers show promising features and variability, as they can be synthesized with tailor-made physical and chemical properties (architectures and functionalisation). In the field of polymer-based gene delivery, the tuning potential of polymers by using different architectures like graft and star-shaped polymers as well as self-assembled block copolymers is immense. In particular, in the last few years numerous new polymer designs showed enhanced transfection properties in combination with good biocompatibility. Furthermore, new insights into the transfection mechanism demonstrated the continuous progress in this field. Polymer architecture influences the polyplex characteristics and the latter has an impact on the transfection mechanism, e.g. the interaction with the cellular membrane depends on the polyplex shape. Moreover, polyplex dissociation can be easily influenced by the polymer chemistry, thus biodegradable linkers lead to well suited polymers with reduced toxicity and high delivery potential, and are also promising for in vivo applications. This review focuses on the influence of polymer architectures for pDNA transfection in vitro, showing recent developments and insights. The theoretical background concerning the biological challenges for cationic polymers and the impact of graft-or star-shaped architectures as well as self-assembled structures will be presented in detail.

show abstract

Section: Star-shaped Polymersmentioning

confidence: 99%

The influence of polymer architecture on in vitro pDNA transfection

et al. 2015

View full text Add to dashboard Cite

show abstract

“…29 Lipofectamine ® 2000 was purchased from Thermo Fisher Scientific (Waltham, MA, USA). MMP-9-siRNA was purchased from Shanghai GenePharma Co., Ltd., Shanghai, People's Republic of China.…”

Section: Materials and Methods Materialsmentioning

confidence: 99%

Cationic star-shaped polymer as an siRNA carrier for reducing MMP-9 expression in skin fibroblast cells and promoting wound healing in diabetic rats

Luo

Yang

et al. 2014

IJN

Self Cite

View full text Add to dashboard Cite

Background Excessive expression of matrix metalloproteinase-9 (MMP-9) is deleterious to the cutaneous wound-healing process in the context of diabetes. The aim of the present study was to explore whether a cationic star-shaped polymer consisting of β-cyclodextrin (β-CD) core and poly(amidoamine) dendron arms (β-CD-[D 3 ] 7 ) could be used as the gene carrier of small interfering RNA (siRNA) to reduce MMP-9 expression for enhanced diabetic wound healing. Methods The cytotoxicity of β-CD-(D 3 ) 7 was investigated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (MMT) method in the rat CRL1213 skin fibroblast cell line. The transfection efficiency of β-CD-(D 3 ) 7 /MMP-9-small interfering RNA (siRNA) complexes was determined by confocal microscopy and flow cytometry. Quantitative real time (RT) polymerase chain reaction was performed to measure the gene expression of MMP-9 after the transfection by β-CD-(D 3 ) 7 /MMP-9-siRNA complexes. The β-CD-(D 3 ) 7 /MMP-9-siRNA complexes were injected on the wounds of streptozocin-induced diabetic rats. Wound closure was measured on days 4 and 7 post-wounding. Results β-CD-(D 3 ) 7 exhibited low cytotoxicity in fibroblast cells, and easily formed the complexes with MMP-9-siRNA. The β-CD-(D 3 ) 7 /MMP-9-siRNA complexes were readily taken up by fibroblast cells, resulting in the downregulation of MMP-9 gene expression ( P <0.01). Animal experiments revealed that the treatment by β-CD-(D 3 ) 7 /MMP-9-siRNA complexes enhanced wound closure in diabetic rats on day 7 post-wounding ( P <0.05). Conclusion β-CD-(D 3 ) 7 may be used as an efficient carrier for the delivery of MMP-9-siRNA to reduce MMP-9 expression in skin fibroblast cells and promote wound healing in diabetic rats.

show abstract

“…Moreover, the signal at 7.84 ppm suggested the presence of the triazole proton, which was attributed to the formation of PP-PLLD by Cu(I)-catalyzed Huisgen 1,3-dipolar cycloaddition of PP-N 3 with PLLD-G3. [ 30 ] Particularly, the integral ratio (I d :I f ) of the proton resonance signal at 2.70 ppm to that at 3.87 ppm was found to be about 1:1, which indicated that 1 mol of PP-N 3 reacted with 4 mol of PLLD-G3, confi rming the intact star-shaped structure of PP-PLLD.…”

Section: Introductionmentioning

confidence: 87%

Star‐Shaped Polymer Consisting of a Porphyrin Core and Poly(L‐lysine) Dendron Arms: Synthesis, Drug Delivery, and In Vitro Chemo/Photodynamic Therapy

Liu

Qin

et al. 2013

Macromol. Rapid Commun.

View full text Add to dashboard Cite

A novel star-shaped polymer, porphyrin-poly(L-lysine) dendrons (PP-PLLD), is synthesized by the click reaction between azido-modified porphyrin and propargyl focal point poly(L-lysine) dendrons. Its chemical structure is characterized by (1) H nuclear magnetic resonance, Fourier transform infrared spectroscopy, and gel permeation chromatography (GPC) is analyses etc. Due to its amphiphilic property, the obtained PP-PLLD has a low critical micelle concentration in an aqueous solution, and can load doxorubicin (DOX) with a loading amount of 64 μg mg(-1) . By in vitro toxicity assay, PP-PLLD has no dark cytotoxicity but has significant phototoxicity. Moreover, DOX-loaded PP-PLLD shows a higher cytotoxicity under the light condition than PP-PLLD or DOX alone, suggesting PP-PLLD has a potential application in combined photodynamic therapy and chemotherapy.

show abstract

Star-shaped polymers consisting of a β-cyclodextrin core and poly(amidoamine) dendron arms: binding and release studies with methotrexate and siRNA

Cited by 50 publications

References 39 publications

The influence of polymer architecture on in vitro pDNA transfection

The influence of polymer architecture on in vitro pDNA transfection

Cationic star-shaped polymer as an siRNA carrier for reducing MMP-9 expression in skin fibroblast cells and promoting wound healing in diabetic rats

Star‐Shaped Polymer Consisting of a Porphyrin Core and Poly(L‐lysine) Dendron Arms: Synthesis, Drug Delivery, and In Vitro Chemo/Photodynamic Therapy

Contact Info

Product

Resources

About