Starch and Sugar Metabolism Response to Post-Anthesis Drought Stress During Critical Periods of Elite Wheat (Triticum aestivum L.) Endosperm Development

Li, Chao; Fu, Kaiyong; Guo, Wenting; Zhang, Xiangchi; Li, Chunyan; Li, Cheng

doi:10.1007/s00344-023-10930-3

Cited by 15 publications

(3 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies on wheat species have shown that under drought stress, the soluble sugar content and sucrose synthase (SS) activity significantly increase in the endosperm of these two plant species. The sucrose content and total starch content also increase under drought treatment (Li et al, 2023b). Starch and sucrose metabolism play important roles in providing quinoa with drought resistance (Huan et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus)

Chen,

Zhong,

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

IntroductionWatermelon is an annual vine of the family Cucurbitaceae. Watermelon plants produce a fruit that people love and have important nutritional and economic value. With global warming and deterioration of the ecological environment, abiotic stresses, including drought, have become important factors that impact the yield and quality of watermelon plants. Previous research on watermelon drought resistance has included analyzing homologous genes based on known drought-responsive genes and pathways in other species.MethodsHowever, identifying key pathways and genes involved in watermelon drought resistance through high-throughput omics methods is particularly important. In this study, RNA-seq and metabolomic analysis were performed on watermelon plants at five time points (0 h, 1 h, 6 h, 12 h and 24 h) before and after drought stress.ResultsTranscriptomic analysis revealed 7829 differentially expressed genes (DEGs) at the five time points. The DEGs were grouped into five clusters using the k-means clustering algorithm. The functional category for each cluster was annotated based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database; different clusters were associated with different time points after stress. A total of 949 metabolites were divided into 10 categories, with lipids and lipid-like molecules accounting for the most metabolites. Differential expression analysis revealed 22 differentially regulated metabolites (DRMs) among the five time points. Through joint analysis of RNA-seq and metabolome data, the 6-h period was identified as the critical period for watermelon drought resistance, and the starch and sucrose metabolism, plant hormone signal transduction and photosynthesis pathways were identified as important regulatory pathways involved in watermelon drought resistance. In addition, 15 candidate genes associated with watermelon drought resistance were identified through joint RNA-seq and metabolome analysis combined with weighted correlation network analysis (WGCNA). Four of these genes encode transcription factors, including bHLH (Cla97C03G068160), MYB (Cla97C01G002440), HSP (Cla97C02G033390) and GRF (Cla97C02G042620), one key gene in the ABA pathway, SnRK2-4 (Cla97C10G186750), and the GP-2 gene (Cla97C05G105810), which is involved in the starch and sucrose metabolism pathway.DiscussionIn summary, our study provides a theoretical basis for elucidating the molecular mechanisms underlying drought resistance in watermelon plants and provides new genetic resources for the study of drought resistance in this crop.

show abstract

Section: Discussionmentioning

confidence: 99%

Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus)

Chen,

Zhong,

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…In the current results, drought stress caused a prominent decrease in carbohydrate metabolic enzymes such as Ald, PGM, UGPase, vacInv, G 6 PDH, and cwInv, which might be associated with upregulation of proteins related to α-amylase and β-amylase enzymes. Overexpression of such enzymes is normally responsible for degradation of starch into soluble sugars to increase the cellular accumulation of osmoregulators, thus providing a large amount of energy to plants for optimal cell division and metabolism under drought stress. , A prompt increase in the activities of carbohydrate metabolic enzymes such as Ald, PGM, UGPase, vacInv, G 6 PDH, and cwInv in wheat plants raised from transgenerational seeds exposed to e [CO 2 ] is strongly related to the key role of e [CO 2 ] in suppressing the upregulation of the lichenase enzyme responsible for degradation of carbohydrates within the plant system under drought stress . It is also evident that carbohydrate metabolic enzymes like Ald, PGM, UGPase, vacInv, G 6 PDH, and cwInv have essential roles in drought tolerance in plants .…”

Section: Discussionmentioning

confidence: 99%

Transgenerational Seed Exposure to Elevated CO₂ Involves Stress Memory Regulation at Metabolic Levels to Confer Drought Resistance in Wheat

Shafique Ahmad,

Shehzad,

Javid

et al. 2024

ACS Omega

View full text Add to dashboard Cite

Drought is the worst environmental stress constraint that inflicts heavy losses to global food production, such as wheat. The metabolic responses of seeds produced overtransgenerational exposure to e[CO 2 ] to recover drought's effects on wheat are still unexplored. Seeds were produced constantly for four generations (F1 to F4) under ambient CO 2 (a[CO 2 ], 400 μmol L −1 ) and elevated CO 2 (e[CO 2 ], 800 μmol L −1 ) concentrations, and then further regrown under natural CO 2 conditions to investigate their effects on the stress memory metabolic processes liable for increasing drought resistance in the next generation (F5). At the anthesis stage, plants were subjected to normal (100% FC, field capacity) and drought stress (60% FC) conditions. Under drought stress, plants of transgenerational e[CO 2 ] exposed seeds showed markedly increased superoxide dismutase (16%), catalase (24%), peroxidase (9%), total antioxidants (14%), and proline (35%) levels that helped the plants to sustain normal growth through scavenging of hydrogen peroxide (11%) and malondialdehyde (26%). The carbohydrate metabolic enzymes such as aldolase (36%), phosphoglucomutase (12%), UDP-glucose pyrophosphorylase (25%), vacuolar invertase (33%), glucose-6-phosphate-dehydrogenase (68%), and cell wall invertase (17%) were decreased significantly; however, transgenerational seeds produced under e[CO 2 ] showed a considerable increase in their activities in drought-stressed wheat plants. Moreover, transgenerational e[CO 2 ] exposed seeds under drought stress caused a marked increase in leaf Ψ w (15%), chlorophyll a (19%), chlorophyll b (8%), carotenoids (12%), grain spike (16%), hundred grain weight (19%), and grain yield (10%). Hence, transgenerational seeds exposed to e[CO 2 ] upregulate the drought recovery metabolic processes to improve the grain yield of wheat under drought stress conditions.

show abstract

“…To gauge drought tolerance, scientists have examined how plant cell membranes hold up and function under water stress [114]. Early drought stress during grain filling can lower nutrient transport, reducing cell count and metabolic activity in the endosperm [115]. Cysteine proteinase's vital role in plant signaling, growth, development, and stress response was highlighted by [116].…”

Section: Nurturing Drought Resilience In Wheat: Insights Into Physiol...mentioning

confidence: 99%

Exploring Drought Tolerance in Wheat: Insights from Biochemical, Morphological, and Physiological Responses

Hashmi,

Ahmad,

Ahmad

et al. 2023

AJRCS

View full text Add to dashboard Cite

This review paper delves into the intricate biochemical foundations underlying drought tolerance mechanisms in wheat plants. This exploration encompasses multifaceted aspects, ranging from physiological adaptations to gene expression modulation. Plants like wheat employ repertoire of biochemical strategies to bolster their resilience against drought stress. It involves coordinating antioxidant enzymes, osmotic regulators, polyamines, and hormones to combat drought stress. The antioxidant system is crucial, countering reactive oxygen species produced during drought. Enzymes like SOD, CAT, and POD are activated to protect cells. Osmolytes like sugars and polyamines maintain cell integrity and water retention, while hormones like ABA control stomatal closure and water conservation. Gene expression highlights the plant's drought adaptability. AP2/ERF factors boost drought tolerance when overexpressed. Gene repression uses motifs like EAR to reduce expression for environmental adaptation. Gene expression shows the plant's drought adaptability. Overexpressed AP2/ERF factors enhance tolerance. Repression, like the EAR motif, aids variation. The morphological basis study examines how water scarcity affects different growth stages of wheat, such as germination, tillering, flowering, and grain filling. It identifies physio-morphological traits that could serve as the indicators of drought resilience, providing a new way to breed stress resistance. Furthermore, this article also explains plant characteristics important for adapting to drought, including photosynthesis, water relations, nutrient uptake, oxidative state, osmotic balance, and hormonal consequences. Each facet contributes to the intricate web of physiological adaptations that allow wheat plants to withstand and thrive under drought conditions. Comprehending these mechanisms aids breeding for drought-tolerant wheat, ensuring food security amid climate change.

show abstract

Starch and Sugar Metabolism Response to Post-Anthesis Drought Stress During Critical Periods of Elite Wheat (Triticum aestivum L.) Endosperm Development

Cited by 15 publications

References 65 publications

Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus)

Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus)

Transgenerational Seed Exposure to Elevated CO₂ Involves Stress Memory Regulation at Metabolic Levels to Confer Drought Resistance in Wheat

Exploring Drought Tolerance in Wheat: Insights from Biochemical, Morphological, and Physiological Responses

Contact Info

Product

Resources

About

Starch and Sugar Metabolism Response to Post-Anthesis Drought Stress During Critical Periods of Elite Wheat (Triticum aestivum L.) Endosperm Development

Cited by 15 publications

References 65 publications

Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus)

Joint transcriptomic and metabolomic analysis provides new insights into drought resistance in watermelon (Citrullus lanatus)

Transgenerational Seed Exposure to Elevated CO2 Involves Stress Memory Regulation at Metabolic Levels to Confer Drought Resistance in Wheat

Exploring Drought Tolerance in Wheat: Insights from Biochemical, Morphological, and Physiological Responses

Contact Info

Product

Resources

About

Transgenerational Seed Exposure to Elevated CO₂ Involves Stress Memory Regulation at Metabolic Levels to Confer Drought Resistance in Wheat