Starch branching enzymes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Comparative analyses of enzyme structure and gene expression

Mutisya, Joel; Sathish, P.; Sun, Chuanxin; Andersson, Lena; Ahlandsberg, Staffan; Baguma, Yona; Palmqvist, Sara; Odhiambo, Benjamin; Åman, Per; Jansson, Christer

doi:10.1078/0176-1617-00960

Cited by 40 publications

(43 citation statements)

References 39 publications

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“…1). The four regions implicated in the catalytic site of amylolytic enzymes (Jespersen et al, 1993) are conserved in sorghum SBEIIA (data not shown; see Mutisya et al, 2003, for a discussion on sorghum SBEIIB). The principal difference between the two enzymes is the 130 amino 13 acids-long N-terminal sequence of SBEIIB (Fig.…”

Section: Sequence Analysis Of Sorghum Sbeiiamentioning

confidence: 99%

“…Total genomic DNA was isolated from young sorghum leaves as described by Mutisya et al (2003). To determine the SBEIIA gene copy number, approximately 20 µg DNA was digested with the restriction enzymes HpaI, Kpn1 and SacI that cut only once within the probe.…”

Section: Dna and Rna Blot Analysesmentioning

confidence: 99%

“…Screening of the sorghum genomic library (SB-BBc; Mutisya et al, 2003) for SBEIIA was performed with heterologous barley probes.…”

Section: Molecular Cloning and Dna Sequence Analysismentioning

confidence: 99%

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Diurnal oscillation of SBE expression in sorghum endosperm

Mutisya

Sun

Rosenquist

et al. 2009

Journal of Plant Physiology

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Spatial and temporal expression patterns of the sorghum SBEI, SBEIIA and SBEIIB genes, encoding, respectively, starch branching enzyme (SBE) I, IIA and IIB, in the developing endosperm of sorghum (Sorghum bicolor) were studied. Full-length genomic and cDNA clones for sorghum was cloned and the SBEIIA cDNA was used together with gene-specific probes for sorghum SBEIIB and SBEI. In contrast to sorghum SBEIIB, which was expressed primarily in endosperm and embryo, SBEIIA was expressed also in vegetative tissues. All three genes shared a similar temporal expression profile during endosperm development, with a maximum activity at 15-24 days after pollination. This is different from barley and maize where SBEI gene activity showed a significantly later onset compared to that of SBEIIA and SBEIIB. Expression of the three SBE genes in the sorghum endosperm exhibited a diurnal rhythm during a 24-h cycle.

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Section: Sequence Analysis Of Sorghum Sbeiiamentioning

confidence: 99%

Section: Dna and Rna Blot Analysesmentioning

confidence: 99%

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Diurnal oscillation of SBE expression in sorghum endosperm

Mutisya

Sun

Rosenquist

et al. 2009

Journal of Plant Physiology

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“…Because SSs are large and rather poorly expressed proteins, only partial sequences of the corresponding cDNAs were recovered (Table I). Three SBE cDNAs were identified, each almost identical with the cloned SBEI (Mutisya et al, 2003) and SBEIIa and SBEIIb (Sun et al, 1998) transcripts. Three different ISA cDNA sequences, one full and two partial, were identified, while one partial sequence was homologous with PUL cDNA (Burton et al, 1999(Burton et al, , 2002a.…”

Section: Localization Of Starch In the Developing Barley Grainmentioning

confidence: 99%

“…Each individual SS isoform is believed to have a unique function in amylopectin synthesis Li et al, 2003;Morell et al, 2003;Morell and Myers, 2005). Distinct SBE isoforms are recruited for starch synthesis during particular stages of seed filling in barley (Mutisya et al, 2003). Plant DBEs are categorized into the isoamylase (ISA) and the pullanase (or b-limit dextrinase; PUL) types.…”

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confidence: 99%

Spatiotemporal Profiling of Starch Biosynthesis and Degradation in the Developing Barley Grain

Borisjuk²,

et al. 2009

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Barley (Hordeum vulgare) grains synthesize starch as the main storage compound. However, some starch is degraded already during caryopsis development. We studied temporal and spatial expression patterns of genes coding for enzymes of starch synthesis and degradation. These profiles coupled with measurements of selected enzyme activities and metabolites have allowed us to propose a role for starch degradation in maternal and filial tissues of developing grains. Early maternal pericarp functions as a major short-term starch storage tissue, possibly ensuring sink strength of the young caryopsis. Gene expression patterns and enzyme activities suggest two different pathways for starch degradation in maternal tissues. One pathway possibly occurs via a-amylases 1 and 4 and b-amylase 1 in pericarp, nucellus, and nucellar projection, tissues that undergo programmed cell death. Another pathway is deducted for living pericarp and chlorenchyma cells, where transient starch breakdown correlates with expression of chloroplast-localized b-amylases 5, 6, and 7, glucan, water dikinase 1, phosphoglucan, water dikinase, isoamylase 3, and disproportionating enzyme. The suite of genes involved in starch synthesis in filial starchy endosperm is much more complex than in pericarp and involves several endosperm-specific genes. Transient starch turnover occurs in transfer cells, ensuring the maintenance of sink strength in filial tissues and the reallocation of sugars into more proximal regions of the starchy endosperm. Starch is temporally accumulated also in aleurone cells, where it is degraded during the seed filling period, to be replaced by storage proteins and lipids.

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Comparative proteomic based analysis of grain nutrients in two hulless barley cultivars

et al. 2021

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Hullessbarley (Hordeum vulgare L.) is a barley cultivar, serving as one of the major food crops worldwide. The main nutrients in hulless barley are starch, protein, and βglucan, the contents of which vary considerably among different cultivars. However, the potential molecular mechanisms that underlie these variations in nutrient are still insufficiently clear. In this study, the nutrient content of two hulless barley cultivars ('Zangqing 2000' and 'C2') was analyzed, and quantitative tandem mass tag-LC-MS/MS proteomics and bioinformatics analysis were used to verify the differentially expressed proteins (DEPs) between the two cultivars. Parallel reaction monitoring (PRM) analysis was used to verify the expression change of candidate DEPs. The content of starch and amylose in C2 was lower than that in Zangqing 2000, and βglucan, protein content, and amylopectin showed a higher concentration in C2 hulless barley cultivar. A total of 326 DEPs were identified, with 108 induced proteins and 218 reduced proteins in C2 compared with Zangqing 2000. Further functional and pathways analysis of DEPs revealed that C2 hulless barley cultivar has relatively more biological activities, and DEPs in C2 hulless barley cultivar were mainly associated with the biological processes of multiple important metabolic pathways. Moreover, PRM analysis showed the accuracy level of quantitative proteomic data. Overall, this was the first study that undertook the proteomic analysis in Zangqing 2000 and C2 and the results revealed the molecular mechanisms of seed nutrient biosynthesis and accumulation, which provide key insights about the development of hulless barley quality.

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Starch branching enzymes in sorghum (Sorghum bicolor) and barley (Hordeum vulgare): Comparative analyses of enzyme structure and gene expression

Cited by 40 publications

References 39 publications

Diurnal oscillation of SBE expression in sorghum endosperm

Diurnal oscillation of SBE expression in sorghum endosperm

Spatiotemporal Profiling of Starch Biosynthesis and Degradation in the Developing Barley Grain

Comparative proteomic based analysis of grain nutrients in two hulless barley cultivars

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