Starch Extraction and Amylose Analysis from Half Seeds

Mohammadkhani, Ali; Stoddard, Frederick L.; Marshall, D. R.; Uddin, M. Nizam; Zhao, Xiaoyan

doi:10.1002/(sici)1521-379x(199903)51:2<62::aid-star62>3.3.co;2-7

Cited by 10 publications

(11 citation statements)

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“…SDS‐PAGE analysis of starch granule proteins: The isolation of starch granules from half seeds and the separation of starch granule–bound proteins followed the method reported in the study by Zhao and Sharp (1996) with some modifications, as in the study by Mohammadkhani et al. (1999).…”

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confidence: 99%

Amylose content is not affected by overexpression of the Wx‐B1 gene in durum wheat

Sestili¹,

Botticella²,

Proietti³

et al. 2012

Plant Breeding

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With 6 figures and 1 table Abstract The granule‐bound starch synthase (GBSS), known as waxy protein, is the sole enzyme responsible for amylose synthesis, whereas amylopectin is produced by concerted action of several isoforms, including starch synthases, branching and debranching enzymes. Durum wheat contains two waxy genes, which encode the Wx‐A1 and Wx‐B1 proteins, respectively. In order to investigate the effect of the overexpression of the waxy genes on amylose content and starch properties, durum wheat transgenic lines overexpressing the Wx‐B1 gene were produced. Amylose content of the transgenic lines did not show any increase compared to the untransformed cultivar. One of the transgenic lines was crossed with a mutant waxy genotype of durum wheat lacking all the waxy protein isoforms. The screening of F2 segregant plants permitted the identification of lines possessing various combinations of waxy genes, including the line containing only the construct used in the transformation. In this line, the normal vitreous seed phenotype, because of the presence of amylose, was restored confirming a correct functioning of the transgene.

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mentioning

confidence: 99%

Amylose content is not affected by overexpression of the Wx‐B1 gene in durum wheat

Sestili¹,

Botticella²,

Proietti³

et al. 2012

Plant Breeding

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“…Starch granules were purified from mature seeds and starch granule proteins were separated by SDS-PAGE following the method reported in Zhao and Sharp (1996) with some modifications (Mohammadkhani et al, 1999). Protein bands were visualized by silver staining.…”

Section: Detection Of Ftgbssi Protein By Sodium Dodecyl Sulfate Polyamentioning

confidence: 99%

Identification and characterization of granule bound starch synthase I (GBSSI) gene of tartary buckwheat (Fagopyrum tataricum Gaertn.)

Wang

Feng

et al. 2014

Gene

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“…Atypical types of amylopectin bind more iodine and give higher La, than normal amylopectin, so that they give an in-69 1 (2) amylopectins occur in several legume and tuber starches; and type (3) has been reported in sweet potato starches (Takedaet al, 1986). The authors have used the methods cited in the literature (McCready and Hassid, 1943;Williams et al, 1970;Juliano, 1971;Morrison and Laignelet, 1983;Knutson, 1986;Yun and Matheson, 1990;Sen at al., 1997;Mohammadkhani et al, 1999) for the iodometric estimation of amylose content of cereal, tuber, and legume starches. The authors' experience has shown that none of the above methods yield reproducible results.…”

Section: Background Informationmentioning

confidence: 99%