Starch-Gel Electrophoresis of Mouse Pituitary Gonadotrophins

Lloyd, Helen; Bindon, B. M.; Lamond, D. R.; Meares, J. D.

doi:10.1677/joe.0.0400313

Cited by 4 publications

(5 citation statements)

References 19 publications

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“…The simi¬ larity between the rat FSH levels reported in the present paper and those reported by others using the Steelman & Pohley (1953) assay for FSH (Greenwald, 1966;Labhsetwar, 1967;Goldman & Mahesh, 1968) provides indirect evidence that the present method is specific for FSH. Further, the levels of FSH found for the mouse pituitary agree well with those reported recently by Lloyd, Bindon, Lamond & Meares (1968) after purification of mouse FSH by starch gel electrophoresis.…”

Section: Discussionsupporting

confidence: 90%

Follicle-Stimulating Hormone Content of the Pituitary Gland Before Implantation in the Mouse and Rat

Bindon¹

1969

Journal of Endocrinology

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The method of Lamond & Bindon (1966) was used to measure follicle\x=req-\ stimulating hormone (FSH) in the pituitary glands of mice and rats killed at different times on each of days 1\ p=n-\ 6 of pregnancy. The sensitivity of the method allowed detection of FSH fluctuations not previously described in these species. A significant fall in pituitary FSH occurred in both species on day 3, although it was earlier in the mouse than in the rat. This transient decline in FSH activity, interpreted as a release of the hormone, was followed in both species by a gradual increase on days 4 and 5. Ovarian weight, measured only in the mouse, increased significantly late on day 3 and is believed to reflect changes in pituitary FSH.The results indicate that implantation in the mouse and rat is initiated by altered activity of the pituitary gland during the first 3 days of pregnancy. It appears that this pituitary mechanism differs from that responsible for the events of the oestrous cycle.

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Section: Discussionsupporting

confidence: 90%

Follicle-Stimulating Hormone Content of the Pituitary Gland Before Implantation in the Mouse and Rat

Bindon¹

1969

Journal of Endocrinology

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“…In addition to his work at that time with cattle and sheep, he and Don Lamond collaborated in some fundamental endocrinological studies, and some elegant and very useful techniques for bioassays of pituitary hormones were developed using hypophysectomised mice as the model Bindon 1966a, 1966b;Lloyd et al 1968).…”

Section: Early Careermentioning

confidence: 99%

“…Work before the Beef CRC After B. M. Bindon commenced research focused on understanding the control of pituitary concentrations of gonadotrophins in laboratory animals (Lloyd et al 1968), he initiated a line of study assessing how blood concentrations of the gonadotrophins, FSH and LH, were regulated in sheep with enhanced fecundity (Bindon 1972;Bindon and Turner 1974;Bindon et al 1971;Findlay and Bindon 1976). There was a growing body of scientific evidence, to which he made significant contributions, that the ovarian factor, inhibin, was a primary regulator of blood concentrations of FSH.…”

Section: Professional Scientific and Industry Contributionsmentioning

confidence: 99%

Bernard Michael Bindon — reproductive physiologist, animal scientist, research leader

Entwistle

Cummins

Hillard

et al. 2006

Aust. J. Exp. Agric.

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This paper is a foreword to a series of papers commissioned on ‘the impact of science on the beef industry’, where the Beef CRC-related collaborative scientific work of Professor Bernard Michael Bindon will be reviewed. These papers will be presented in March 2006, as part of a ‘festschrift’ to recognise his wider contributions to the Australian livestock industries for over 40 years. Bindon’s career involved basic and applied research in many areas of reproductive physiology, genetics, immunology, nutrition, meat science and more recently genomics, in both sheep and cattle. Together with his collaborators, he made large contributions to animal science by improving the knowledge of mechanisms regulating reproductive functions and in elucidating the physiology and genetics of high fecundity livestock. His collaborative studies with many colleagues of the reproductive biology and genetics of the Booroola Merino were amongst the most extensive ever conducted on domestic livestock. He was instrumental in the development of immunological techniques to control ovulation rate and in examining the application of these and other techniques to increase beef cattle reproductive output. This paper tracks his investigations and achievements both within Australia and internationally. In the later stages of his career he was the major influence in attracting a large investment in Cooperative Research Centres for the Australian cattle industry, in which he directed a multi-disciplinary approach to investigate, develop and disseminate science and technology to improve commercial cattle productivity.

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“…Human thyrotropin is more electronegative than porcine and bovine forms because it has a greater sialic acid content (1180). The gonadotropic activity in starchgel electrophoregrams of mouse pituitary homogenates has been located (782). Pig follicle-stimulating hormone can be assayed by immunoelectrophoresis (1258).…”

Section: Enzymesmentioning

confidence: 99%

“…The gonadotropic activity in starchgel electrophoregrams of mouse pituitary homogenates has been located (782).…”

Section: Microorganismsmentioning

confidence: 99%

Electrophoresis

Strickland¹

1970

Anal. Chem.

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Starch-Gel Electrophoresis of Mouse Pituitary Gonadotrophins

Cited by 4 publications

References 19 publications

Follicle-Stimulating Hormone Content of the Pituitary Gland Before Implantation in the Mouse and Rat

Follicle-Stimulating Hormone Content of the Pituitary Gland Before Implantation in the Mouse and Rat

Bernard Michael Bindon — reproductive physiologist, animal scientist, research leader

Electrophoresis

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