This chapter describes the overview of Web 2.0 technologies; Web 2.0 applications in learning and education; Web 2.0 applications in academic libraries; Web 2.0 applications in Knowledge Management (KM); the perspectives of Health Information Technology (health IT); the multifaceted applications of health IT; IT and Technology Acceptance Model (TAM); and the significance of health IT in the health care industry. Web 2.0 is the platform of the network which spans all connected services so that users can utilize them more efficiently. Web 2.0 technologies have various benefits by enhancing the opportunities for business collaboration and by sharing knowledge through online communities of practice toward gaining improved organizational performance. Health IT includes utilizing technology to electronically store, protect, retrieve, and transfer the information in modern health care. Health IT has great potential to improve the quality, safety, and efficiency of health care services in the health care industry.