Starvation for Reduced Nitrogen Stimulates the Secretion of Proteases by Maize Endosperm Cultures

Miernyk, Ján A.; Sturch, Stacey

doi:10.1016/s0176-1617(11)80273-7

Cited by 2 publications

(3 citation statements)

References 22 publications

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“…How this might be related to normal soybean cellular protein trafficking or beneficial to the suspensioncultured cells remains obscure, but it is not without precedent. It is well-known that phosphate starvation leads to secretion of phosphatases from both intact [65,66) and suspension-cultured [18] plant cells, and it has also been shown that starvation for reduced nitrogen can lead to secretion of proteases by maize endosperm suspension cultures [19]. Furthermore, exposure of A. thaliana suspension cells to extracellular proteins from Pseudomonas syringae led to changes in the plant secretome in response to exposure to proteins from a virulent pathovar of the bacterium [22].…”

Section: Discussionmentioning

confidence: 99%

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Analysis of soybean tissue culture protein dynamics using difference gel electrophoresis

Miernyk

Jett

Johnston

2016

Journal of Proteomics

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Section: Discussionmentioning

confidence: 99%

“…The proteome of plant cells is dynamic, changing in response to developmental programs [12][13][14], environmental stress [15][16][17], nutrition [18,19], and host:pathogen interactions [20][21][22][23]. The changes in response to developmental cues typically involve complex networks mediated by signaling pathways [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Analysis of soybean tissue culture protein dynamics using difference gel electrophoresis

Miernyk

Jett

Johnston

2016

Journal of Proteomics

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“…In our protocol, 1% BSA is essential to maintain protoplast viability, an observation suggesting that proteases might be present in the endosperm protoplast preparations that could compromise the integrity of the endosperm plasmalemma. Several protease activities are known to be secreted in endosperm cultures, and cells starved for nitrogen can be rescued by the addition of BSA to the medium (Miernyk and Sturch, 1991). No such BSA requirement was necessary to maintain aleurone protoplast viability.…”

Section: Isolation and Transformation Of Aleurone And Endosperm Protomentioning

confidence: 99%

The Regulation of Gene Expression in Transformed Maize Aleurone and Endosperm Protoplasts (Analysis of Promoter Activity, Intron Enhancement, and mRNA Untranslated Regions on Expression)

Gallie

Young

1994

Plant Physiol.

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Gene expression in the aleurone and endosperm is highly regulated during both seed development and germination. Studies of a-amylase expression in the aleurone of barley (Hordeum vulgare) have generated the current paradigm for hormonal control of gene expression in germinating cereal grain. Gene expression studies in both the aleurone and endosperm tissues of maize (Zea mays) seed have been hampered because of a lack of an efficient transformation system. We report here the rapid isolation of protoplasts from maize aleurone and endosperm tissue, their transformation using polyethylene glycol or electroporation, and the regulation of gene expression in these cells. Adhl promoter activity was reduced relative to the 35s promoter in aleurone and endosperm protoplasts compared to Black Mexican Sweet suspension cells in which it was nearly as strong as the 35s promoter. Intron-mediated stimulation of expression was substantially higher in transformed aleurone or endosperm protoplasts than in cell-suspension culture protoplasts, and the data suggest that the effect of an intron may be affected by cell type. To examine cytoplasmic regulation, the 5' and 3' untranslated regions from a barley a-amylase gene were fused to the firefly luciferase-coding region, and their effect on translation and mRNA stability was examined following the delivery of in vitro synthesized mRNA to aleurone and endosperm protoplasts. The aamylase untranslated regions regulated translational efficiency in a tissue-specific manner, increasing translation in aleurone or endosperm protoplasts but not in maize or carrot cell-suspension protoplasts, in animal cells, or in in vitro translation lysates.The aleurone and endosperm play an essential role in storing reserves during cereal seed development as well as mobilizing those reserves during germination. The expression of several genes in the aleurone is induced following imbibition. a-Amylase synthesized and secreted from the aleurone during germination is involved in the hydrolysis of the starch reserves. The genes encoding a-amylase in barley (Hordeum vulgare) have been extensively characterized as an example of a gene family subject to hormonal regulation.

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Starvation for Reduced Nitrogen Stimulates the Secretion of Proteases by Maize Endosperm Cultures

Cited by 2 publications

References 22 publications

Analysis of soybean tissue culture protein dynamics using difference gel electrophoresis

Analysis of soybean tissue culture protein dynamics using difference gel electrophoresis

The Regulation of Gene Expression in Transformed Maize Aleurone and Endosperm Protoplasts (Analysis of Promoter Activity, Intron Enhancement, and mRNA Untranslated Regions on Expression)

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