Starvation-Induced Activity in Cockroach Malpighian Tubules1

Meyers, John; Miller, Thomas R.

doi:10.1093/aesa/62.4.725

Cited by 19 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All the experiments that involved PBAN stimulation of pheromone production were performed 5 h before lights-off. Females were anesthetized by brief cooling and injected with 4 p1 of a PBAN solution in Meyers and Miller's saline (Meyers and Miller, 1969) containing the doses indicated. To study the effect of PBAN on the incorporation of d3 14:Acid into pheromone, the glands were topically treated with 1 pg of d314:Acid in DMSO (0.1 pl), and the insects were released 15 min later and injected with 4 p1 of either saline or saline containing PBAN (12.5 pmols/female).…”

Section: Treatmentsmentioning

confidence: 99%

Inhibitory effect of 10,11‐methyl‐enetetradec‐10‐enoic acid on a Z9‐desaturase in the sex pheromone biosynthesis of Spodoptera littoralis

Gosalbo

Fabriás

Camps

1994

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

The effect of 10,ll -methylenetetradec-l O-enoic acid on the sex pheromone biosynthetic pathway of Spodoptera littoralis is reported. This new cyclopropene fatty acid inhibited the biosynthesis of the main pheromone component from labeled myristic acid. The study of each Z desaturation step revealed that the Z9-desaturase of E l 1-14:Acid was inhibited, whereas the Z11-desaturase of 16:Acid was not affected. The results presented in this article agree with our hypothesis that the methylenehexadecenoic acids are beta-oxidized in the pheromone gland to the corresponding methylenetetradecenoic acids. (0 1994 WiIey-Liss, Inc.

show abstract

Section: Treatmentsmentioning

confidence: 99%

Inhibitory effect of 10,11‐methyl‐enetetradec‐10‐enoic acid on a Z9‐desaturase in the sex pheromone biosynthesis of Spodoptera littoralis

Gosalbo

Fabriás

Camps

1994

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

show abstract

“…AKH-like immunohistochemistry. Immunohistochemistry was performed on tissues dissected under physiological saline (Meyers & Miller, 1969). Tissues were fixed in 2% paraformaldehyde' in Millonig's buffer and whole mounts processed for AKH-like immuno-reactivity (Lange et al, 1988).…”

Section: Methodsmentioning

confidence: 99%

Adipokinetic hormone of the true armyworm, Pseudaletia unipuncta: immunohistochemistry, amino acid analysis, quantification and bioassay

Orchard

Cusson

McNeil

1991

Physiological Entomology

View full text Add to dashboard Cite

A cluster of five to seven AKH‐like immunoreactive cells lie in each lobe of the paired corpora cardiaca of the true armyworm, Pseudaletia unipuncta. These cells form a mesh work of immunoreactive processes within the corpora cardiaca, and immunoreactive tracts projecting posteriorly over the aorta. Reversed‐phase high performance liquid chromatography of extracts of the corpora cardiaca of P.unipuncta revealed a single large U.V. absorbent peak with a retention time identical to synthetic Manduca‐AKH. Amino acid analysis of the contents of this peak yielded a composition identical to that of synthetic Manduca‐AKH which was analysed in a parallel manner. Furthermore the material within the peak possessed adipokinetic activity when bioassayed in day 2 adult male P. unipuncta. The corpora cardiaca of similar individuals were found to contain approximately 17.6ng (17.6pmol) of Manduca‐AKH equivalents per pair. Injection of Manduca‐AKH into 2‐day‐old adult male P.unipuncta resulted in a dose‐dependent elevation in haemolymph lipid levels with a maximum level of 80–90μmg/μl obtained with 5–10 ng of Manduca‐AKH. Continuous flight also elevated haemolymph lipid levels in day 4 adult males with a significant elevation evident in the first samples taken after 15 min of flight and lipid levels plateauing at approximately 100 μg/μl by about 60 min of flight.

show abstract

“…Preliminary observations indicated that 90 min post-eclosion was the earliest that a successful injection could be made, and this time was used in all tissue injection assays. All injections were made through the dorsal surface of the abdomen with 15 pl of saline only (Meyers & Miller, 1969), or with one insect equivalent of an appropriate tissue homogenate in 15 +I of saline. 'Total weight loss was measured 4.5 h after injection (6 h after emergence).…”

Section: Methodsmentioning

confidence: 99%

Post‐eclosion diuresis in adult Heliothis zea

Bushman

Raina

Nelson

1989

Physiological Entomology

View full text Add to dashboard Cite

ABSTRACT. The marked weight loss that follows adult eclosion in Heliothis zea (Boddie) (Lepidoptera: Noctuidae) was found to be the result of diuresis. This diuretic weight loss amounted to 18.2% of the emergence weight in females and 22.5% in males. Ligation between head and thorax immediately following emergence resulted in a significant reduction in this weight loss. Injection of homogenates of brain (BR) or suboesophageal ganglion (SOG) partially restored the weight loss in ligated insects, indicating potential neurohormonal regulation of this phenomenon. Because injection of homogenates of nervous and neuroendocrine tissues other than BR and SOG did not result in increased weight loss over controls, the activity present in BR and SOG was probably a tissue‐specific phenomenon. We speculate that a factor at least partially responsible for the enhanced diuretic weight loss is present in the fused BR/SOG.

show abstract

Starvation-Induced Activity in Cockroach Malpighian Tubules1

Cited by 19 publications

References 0 publications

Inhibitory effect of 10,11‐methyl‐enetetradec‐10‐enoic acid on a Z9‐desaturase in the sex pheromone biosynthesis of Spodoptera littoralis

Inhibitory effect of 10,11‐methyl‐enetetradec‐10‐enoic acid on a Z9‐desaturase in the sex pheromone biosynthesis of Spodoptera littoralis

Adipokinetic hormone of the true armyworm, Pseudaletia unipuncta: immunohistochemistry, amino acid analysis, quantification and bioassay

Post‐eclosion diuresis in adult Heliothis zea

Contact Info

Product

Resources

About