Starved human fibroblasts secrete acidic proteins inducing post re‐feeding proliferation and in vitro cell migration: A potential tool for wound healing

Abstract

We concluded that human starved fibroblasts secrete proteins that are able to induce post re-feeding cell proliferation and fibroblasts migration, probably through the induction of a sustained calcium influx. This is worth being considered as a potential tool for wound healing.

GSK-3β controls autophagy by modulating LKB1-AMPK pathway in prostate cancer cells

“…As shown in Figure 1E, GSK-3b inhibitors had only a mild effect on cellular glycolysis activity under FBS-supplied condition. Consistent with a recent report [32], cellular glycolytic activity (in other word, L-lactate release) elevated about two-fold under FBSfree condition, however, GSK-3b inhibitors significantly reduced glycolysis activity in a dose-dependent manner (Fig. 1F).…”

Downregulation of Stemness Genes and Induction of Necrosis in Rat LA7 Cancer Stem Cells Induced Tumors Treated with Starved Fibroblasts Culture Supernatant

“…We supposed that the 16h-SFS solution can also selectively kill CSCs but not normal cells. We have previously shown that 16h-SFS promoted the survival of our body ubiquitous cells, i.e., fibroblasts in serum free condition, and accelerated the post-refeeding proliferation in these cells (28). In the present study, the tumors derived from 16h-SFS treated animals exhibited relatively higher levels of angiogenesis, suggesting that 16h-SFS solution similar to xenobiotics might cause elevation in ROS concentrations in LA7 CSCs.…”

“…We isolated the fibroblasts from fresh foreskin (newborn; n= 3, age; 1 to 1.5 months) based on the method already described (27). The isolated cells were cultured in Dulbecco's Modified Eagle's Medium (DMEM, Biowest, France), supplemented with 10% fetal bovine serum (FBS), (Sigma, Germany), streptomycin (100 µg/mL) and Penicillin (100 IU/mL), at 37 ºC with 5% CO2 saturation, until the cell number reached adequate levels to carry out the serum starvation procedure according to the method already reported (28). Then at 80% confluency in 75 cm 2 cell culture flask, the cells were washed with phosphate buffered saline (PBS) twice, and cell culture was continued under standard conditions for 16 h in serum free, glucose containing DMEM supplemented with streptomycin (100 µg/mL) and Penicillin (100 IU/mL) Finally, the cell culture supernatants were harvested, pooled, and stored at -20 °C until use.…”

Cell death, cavitation and spontaneous multi‐differentiation of dental pulp stem cells‐derived spheroids in vitro: A journey to survival and organogenesis

“…It has been considered that numerous types of cell are able to migrate in response to environmental cues (Zylbersztejn and Galli, ). A new study showed that proteins secreted from starved fibroblasts are able to induce other starved cell proliferation and promote fibroblasts migration in an in vitro model of human skin wound healing test (Golpour et al., ). It is possible that some mature cells simply migrated into better place to get nutrition and oxygen due to environmental changes, such as the secretion from the starving cells.…”