Biology Entrepreneurship Education has become a mandatory course that imparts a distinct characteristic to its graduates. There is an expectation that students will possess an entrepreneurial spirit and immediately establish businesses upon graduation. However, entrepreneurship education often leaves a gap between expectations and real-world outcomes. This research aims to elucidate reflections on the success of education on Entrepreneurial Self-Efficacy. We conducted a case study involving students who have completed the biology entrepreneurship course. Twenty students were selected based on specific criteria for in-depth interviews. The findings indicate that all students reported an enhancement in Entrepreneurial Self-Efficacy, including proficiency in business searching, business planning, business marshalling, and implementation. Additionally, it was observed that a substantial number of college graduates do not pursue entrepreneurship after graduation. Field findings reveal that graduates from entrepreneurial backgrounds are more likely to continue entrepreneurial ventures after graduation. Further research can be conducted to evaluate entrepreneurship education programs focusing on specific post-graduation achievements and the factors influencing graduates in pursuing entrepreneurial endeavours.