State of Antioxidant System and Long-Term Storage of Tobacco Hairy Roots with Constitutive Expression of Glutathione-S-Transferase Gene ATGSTF11

Musin, Kh. G.; Fedyaev, V.V.; Kuluev, B. R.

doi:10.1134/s1021443721040105

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…Plants in the experimental plot were subjected to cold stress of natural origin in November (at the age of 200 days). RNA was extracted from plants when the outdoor temperature dropped to −1 • C, and then to −8 • C. Plants cultivated in the laboratory were not subjected to cold stress, because a sudden decrease of temperature rarely occurs in nature, and in our earlier studies, GSTF11 expression level in A. thaliana did not change after a sudden drop of temperature from 22 • C to 8 • C. The 35S:AtGSTF11 transgenic hairy roots of tobacco at +3 • C were the same as in control plants [41].…”

Section: Stress Treatmentmentioning

confidence: 93%

“…GSTF11 is associated with a response to several types of stress [41][42][43]. To determine whether this gene is involved in general stress response or a specific response, transgenic plants were subjected to additional treatments.…”

Section: Response Of Transgenic Plants To Drought and Cold Stressmentioning

confidence: 99%

“…GSTF11 has been also associated with response to other types of stress, but the data are contradictory. Transgenic hairy roots of tobacco demonstrated increased tolerance to salinity, heat stress and heavy metal pollution due to AtGSTF11 expression [41]. This gene also enhances anthocyanin pigmentation in ornamental crop Euphorbia pulcherrima [42].…”

Section: Introductionmentioning

confidence: 99%

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The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress

Mikhaylova

Khusnutdinov

Shein

et al. 2021

Plants

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Oilseed rape (Brassica napus) is an economically important crop. In a temperate climate, powdery mildew Erysiphe crucifertaum can drastically reduce its yield. Nevertheless, cultivars resistant to this fungal disease have not yet been selected. Glutathione S-transferase GSTF11 is involved in glucosinolate (GSL) biosynthesis and response to stress, including fungal deceases. However, the impact of exogenous GSTF11 gene expression on resistance to powdery mildew has not yet been confirmed and requires further investigation. Transgenic B. napus was generated for this purpose. It demonstrated increased GST activity and a higher GSH:GSSG ratio under normal conditions. Powdery mildew Erysiphe crucifertaum caused 50% mortality in wild type (WT) plants. In most of transgenic plants, mycelium growth was inhibited. The infection contributed to higher GSTF11 expression and increased levels of glutathione (GSH) and oxidized glutathione (GSSG) in both transgenic and WT plants. In contrast, GSTF11 mRNA content, GST activity and GSSG level were lower only in WT plants. In transgenic plants, increased resistance to powdery mildew correlated with a lower GSH:GSSG ratio, indicating a higher content of neutralized toxic molecules. GSTF11 expression was also affected by cold stress, but not drought. At −1 °C, the expression level increased only in transgenic plants. Therefore, GSTF11 appears to be nonspecific and is able to protect plants under several types of stress. This gene could be used as a target in the production of stress tolerant cultivars.

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Section: Stress Treatmentmentioning

confidence: 93%

Section: Response Of Transgenic Plants To Drought and Cold Stressmentioning

confidence: 99%