State-of-Art Bio-Assay Systems and Electrochemical Approaches for Nanotoxicity Assessment

Shinde, Ravikumar B.; Veerapandian, Murugan; Kaushik, Ajeet; Manickam, Pandiaraj

doi:10.3389/fbioe.2020.00325

Cited by 10 publications

(9 citation statements)

References 111 publications

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“…Conventional analytical techniques like microplate reader, cytometer, high content imaging, and spectrophotometric techniques typically usually take a long period of time and often lead to false-positive outcomes. Among various analytical techniques, bioelectrochemical techniques are able to measure the nanotoxic effects ( in vitro and in vivo ) by a noninvasive method, at multicellular as well as unicellular levels ( Shah et al, 2014 ; Shinde et al, 2020 ).…”

Section: Novel Technologies Employed For Toxicity Evaluationmentioning

confidence: 99%

“…Electrochemical impedance spectroscopy (ECIS) is used to track cellular biophysical changes in response to NM interactions. Surface coating and physicochemical electrochemical collision techniques are employed to screen the particle reactivity ( Shinde et al, 2020 ). In regulated settings, electrochemical collision is a newly established approach for fast screening and characterization of particle type, catalytic characteristics, and chemical reactivity.…”

Section: Novel Technologies Employed For Toxicity Evaluationmentioning

confidence: 99%

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Novel Methods and Approaches for Safety Evaluation of Nanoparticle Formulations: A Focus Towards In Vitro Models and Adverse Outcome Pathways

et al. 2021

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Nanotoxicology is an emerging field employed in the assessment of unintentional hazardous effects produced by nanoparticles (NPs) impacting human health and the environment. The nanotoxicity affects the range between induction of cellular stress and cytotoxicity. The reasons so far reported for these toxicological effects are due to their variable sizes with high surface areas, shape, charge, and physicochemical properties, which upon interaction with the biological components may influence their functioning and result in adverse outcomes (AO). Thus, understanding the risk produced by these materials now is an important safety concern for the development of nanotechnology and nanomedicine. Since the time nanotoxicology has evolved, the methods employed have been majorly relied on in vitro cell-based evaluations, while these simple methods may not predict the complexity involved in preclinical and clinical conditions concerning pharmacokinetics, organ toxicity, and toxicities evidenced through multiple cellular levels. The safety profiles of nanoscale nanomaterials and nanoformulations in the delivery of drugs and therapeutic applications are of considerable concern. In addition, the safety assessment for new nanomedicine formulas lacks regulatory standards. Though the in vivo studies are greatly needed, the end parameters used for risk assessment are not predicting the possible toxic effects produced by various nanoformulations. On the other side, due to increased restrictions on animal usage and demand for the need for high-throughput assays, there is a need for developing and exploring novel methods to evaluate NPs safety concerns. The progress made in molecular biology and the availability of several modern techniques may offer novel and innovative methods to evaluate the toxicological behavior of different NPs by using single cells, cell population, and whole organisms. This review highlights the recent novel methods developed for the evaluation of the safety impacts of NPs and attempts to solve the problems that come with risk assessment. The relevance of investigating adverse outcome pathways (AOPs) in nanotoxicology has been stressed in particular.

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Section: Novel Technologies Employed For Toxicity Evaluationmentioning

confidence: 99%

Section: Novel Technologies Employed For Toxicity Evaluationmentioning

confidence: 99%

Novel Methods and Approaches for Safety Evaluation of Nanoparticle Formulations: A Focus Towards In Vitro Models and Adverse Outcome Pathways

et al. 2021

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“…Investigation of short- as well as long-term nanotoxicity has gained considerable attention in this context to ensure the nano-safety aspect. Diverse conventional toxicity assessment platforms, particularly the 2D culture model, have been extensively used to elucidate the landscape of toxicity imparted by diverse nanomaterials along with their underlying mechanisms ( Wani et al, 2011 ; Azhdarzadeh et al, 2015 ; Armstead and Li, 2016 ; Fadeel, 2019 ; Abdelhamid, 2020 ; Shinde et al, 2020 ). However, minute alteration in the physicochemical properties of NPs such as shape, size, charge, crystal structure, surface area, surface functionality, and customization protocol can distinctly change the toxicity profile of any established nano-formulation ( Zhu et al, 2012 ; Jeevanandam et al, 2018 ).…”

Section: Nanotoxicity Assessment Using the Organoid Modelmentioning

confidence: 99%

“…Several in vitro as well as in vivo methods provide the scope for nanotoxicity evaluation in a conventional way ( Figure 2 ; Hillegass et al, 2010 ; Arora et al, 2012 ; Chuang et al, 2013 ; Astashkina et al, 2014 ; Kumar et al, 2017 ; Shinde et al, 2020 ). Cytotoxicity analyses along with observations regarding genotoxicity, inflammation, and oxidative stress in cell cultures are the most convenient tools for in vitro nanotoxicity assessment ( Hillegass et al, 2010 ; Arora et al, 2012 ; Astashkina et al, 2014 ; Kumar et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Organoid Technology: A Reliable Developmental Biology Tool for Organ-Specific Nanotoxicity Evaluation

Prasad

Kumar

Buragohain

et al. 2021

Front. Cell Dev. Biol.

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Engineered nanomaterials are bestowed with certain inherent physicochemical properties unlike their parent materials, rendering them suitable for the multifaceted needs of state-of-the-art biomedical, and pharmaceutical applications. The log-phase development of nano-science along with improved “bench to beside” conversion carries an enhanced probability of human exposure with numerous nanoparticles. Thus, toxicity assessment of these novel nanoscale materials holds a key to ensuring the safety aspects or else the global biome will certainly face a debacle. The toxicity may span from health hazards due to direct exposure to indirect means through food chain contamination or environmental pollution, even causing genotoxicity. Multiple ways of nanotoxicity evaluation include several in vitro and in vivo methods, with in vitro methods occupying the bulk of the “experimental space.” The underlying reason may be multiple, but ethical constraints in in vivo animal experiments are a significant one. Two-dimensional (2D) monoculture is undoubtedly the most exploited in vitro method providing advantages in terms of cost-effectiveness, high throughput, and reproducibility. However, it often fails to mimic a tissue or organ which possesses a defined three-dimensional structure (3D) along with intercellular communication machinery. Instead, microtissues such as spheroids or organoids having a precise 3D architecture and proximate in vivo tissue-like behavior can provide a more realistic evaluation than 2D monocultures. Recent developments in microfluidics and bioreactor-based organoid synthesis have eased the difficulties to prosper nano-toxicological analysis in organoid models surpassing the obstacle of ethical issues. The present review will enlighten applications of organoids in nanotoxicological evaluation, their advantages, and prospects toward securing commonplace nano-interventions.

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“…However, electrochemical based techniques can be proper options since they have the potential to use electrical conductive properties combined with high chemical stable materials at the active detector surface [242] . Thus, these detection or determination techniques seem to have the adequate flexibility to be used in design and fabrication of biosensors for nitrite/nitrate detection in food or biological samples.…”

Section: The Level Of Os and Related Compoundsmentioning

confidence: 99%

Review on oxidative stress relation on COVID-19: Biomolecular and bioanalytical approach

Ebrahimi

Norouzi

Aazami

et al. 2021

International Journal of Biological Macromolecules

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State-of-Art Bio-Assay Systems and Electrochemical Approaches for Nanotoxicity Assessment

Cited by 10 publications

References 111 publications

Novel Methods and Approaches for Safety Evaluation of Nanoparticle Formulations: A Focus Towards In Vitro Models and Adverse Outcome Pathways

Novel Methods and Approaches for Safety Evaluation of Nanoparticle Formulations: A Focus Towards In Vitro Models and Adverse Outcome Pathways

Organoid Technology: A Reliable Developmental Biology Tool for Organ-Specific Nanotoxicity Evaluation

Review on oxidative stress relation on COVID-19: Biomolecular and bioanalytical approach

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