State-of-art review of bacteria-based self-healing concrete: Biomineralization process, crack healing, and mechanical properties

Aytekin, Burcu; Mardani‐Aghabaglou, Ali; Yazıcı, Şemsi

doi:10.1016/j.conbuildmat.2023.131198

Cited by 45 publications

(6 citation statements)

References 199 publications

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“…Similar to temperature, the pH of the environment (construction material in this case) substantially impacts bacterial enzymatic activity. At the microlevel, pH variation affects microbial cells by two different mechanisms: it alters the enzymatic reaction rate and the transport of nutrients into the cell [91]. Changing the pH level outside of its optimum range may slow down or even stop the bacteria's enzymatic activity and CaCO 3 precipitation, as the bacteria will not survive [43].…”

Section: Parameters That Influence Bio-cementationmentioning

confidence: 99%

Advancements in Exploiting Sporosarcina pasteurii as Sustainable Construction Material: A Review

Khoshtinat

2023

Sustainability

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With the development of bioinspired green solutions for sustainable construction over the past two decades, bio-cementation, which exploits the naturally occurring phenomenon of calcium carbonate precipitation in different environments, has drawn a lot of attention in both building construction and soil stabilization. Various types of microorganisms, along with specific enzymes derived from these microorganisms, have been utilized to harness the benefits of bio-cementation. Different application methods for incorporating this mechanism into the production process of the construction material, as well as a variety of experimental techniques for characterizing the outcomes of bio-cementation, have been developed and tested. Despite the fact that the success of bio-cementation as a sustainable method for construction has been demonstrated in a significant body of scientific literature at the laboratory scale, the expansion of this strategy to construction sites and field application remains a pending subject. The issue may be attributed to two primary challenges. Firstly, the complexity of the bio-cementation phenomenon is influenced by a variety of factors. Secondly, the extensive body of scientific literature examines various types of microorganisms under different conditions, leading to a wide range of outcomes. Hence, this study aims to examine the recent advancements in utilizing the most commonly employed microorganism, Sporosarcina pasteurii, to emphasize the significance of influential factors identified in the literature, discuss the findings that have been brought to light, and outline future research directions toward scaling up the process.

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Section: Parameters That Influence Bio-cementationmentioning

confidence: 99%

Advancements in Exploiting Sporosarcina pasteurii as Sustainable Construction Material: A Review

Khoshtinat

2023

Sustainability

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“…The precipitation of CaCO₃ is based on the enzymatic reaction by using urease [23]. According to the research [39], the self-healing ratio resulted 44,71% efficiency of incorporation Jsmartech.ub.ac.id of added bacteria in the concrete. The results of a literature study regarding the ability of ureolytic bacterial species to form endospores (Table 3) indicate that the group of bacteria that has the potential to close cracks in concrete comes from the Bacillus sp.…”

Section: Potential Analysis Of Ureolytic Bacteria Speciesmentioning

confidence: 99%

Phylogenetic Analysis of UreABC Protein in Ureolytic Bacteria as Self-Healing Agent in Concrete

Audia,

Setiawan,

Mora

et al. 2023

JSMARTech

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Concrete is one of Indonesia's most widely used materials in infrastructure development because it is strong enough to withstand pressure, adaptable, and has relatively low maintenance costs. Bacteria are potential agents that can be used to close cracks in concrete. Therefore, this research is aimed to determine which bacteria can produce the enzyme urease based on constructing a phylogenetic tree, the ability to produce spores, and the characteristics of ureolytic bacteria. This study used four sequences of ureolytic bacteria that code for ureABC, namely Bacillus, Clostridium, Pseudomonas, and Enterobacter, with Micrococcus terreus as the outgroup. The four bacterial groups with outgroups were aligned and phylogeny constructed using the Neighbor-Joining (NJ) method with 1000 replication and grouped based on genetic distance in MEGA-X software. Then further screening was carried out based on the ability to form endospores and the characteristics of ureolytic bacteria. Phylogenetic analysis shows that the bacterial groups Bacillus, Enterobacter, Clostridium and Pseudomonas have a fairly close kinship, the construction is based on proteins, genus, cell shape, gram characteristics, and habitat. The ureolytic bacteria group predicted to have the highest potential as a biomaterial agent comes from Bacillus sp. and Clostridium sp. due to its ability to form endospores. Ureolytic activity is indicated by an increased pH value and urea degradation activity due to ammonification with Bacillus sp. having a pH of 6.0-8.0. In contrast, the pH value of Clostridium sp. is unknown because further research is needed in vitro.

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“…This paper explores the utilization of microorganisms in construction materials and the methods employed to evaluate their effect on strength. Concrete, being the most extensively used building material in construction projects (1) is not without its 2 challenges, including the presence of micro cracks (2). These micro cracks can reduce the workability of concrete and potentially lead to structural collapse and failure.…”

Section: Introductionmentioning

confidence: 99%

Harnessing microbes for self-healing concrete – A review

Asgharpour,

Çakiral,

Marar

2024

Res. Eng. Struct. Mat.

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This review explores the burgeoning field of microbially enhanced construction materials, with a special focus on self-healing concrete, through the lens of microbial biotechnology. Central to this discourse is the innovative use of bacteria, particularly Bacillus species, to address the pervasive issue of microcracks in concrete, a fundamental material in the construction industry. Traditional remedies, such as chemical admixtures and fiber reinforcements, offer partial solutions; however, self-healing concrete represents a paradigm shift, harnessing the natural calcite-precipitating ability of bacteria to autonomously repair cracks, thereby augmenting structural durability and longevity. Delving into the mechanics, the bacteria, embedded within the concrete matrix, remain dormant until crack formation triggers their metabolic pathways, leading to calcite production that effectively seals the fissures. This bio-mediated repair mechanism not only enhances the structural integrity of concrete but also aligns with sustainable construction practices by minimizing maintenance requirements and material wastage. The review extends beyond self-healing phenomena, encompassing broader applications of microbial technology in construction, including bio-concrete, bio-cement, and soil stabilization methods. These applications underscore the versatility of microbes in enhancing material properties such as compressive strength, tensile resilience, and water impermeability. Empirical evidence underscores the necessity of optimizing bacterial dosages and curing conditions to maximize the self-healing efficiency. Future research trajectories should aim to elucidate the complex interactions between microbial agents and concrete matrices, assess long-term performance, and evaluate the environmental and economic sustainability of microbial interventions in construction. The integration of microbial technology in construction materials heralds a new epoch of innovation, offering robust, sustainable, and resilient solutions to enduring challenges in the industry.

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State-of-art review of bacteria-based self-healing concrete: Biomineralization process, crack healing, and mechanical properties

Cited by 45 publications

References 199 publications

Advancements in Exploiting Sporosarcina pasteurii as Sustainable Construction Material: A Review

Advancements in Exploiting Sporosarcina pasteurii as Sustainable Construction Material: A Review

Phylogenetic Analysis of UreABC Protein in Ureolytic Bacteria as Self-Healing Agent in Concrete

Harnessing microbes for self-healing concrete – A review

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