State-of-the-art and future perspectives in infertility diagnosis: Conventional versus nanotechnology-based assays

Andone, Bianca-Astrid; Handrea-Dragan, Iuliana M.; Botiz, Ioan; Boca, Sanda

doi:10.1016/j.nano.2023.102709

Cited by 8 publications

(3 citation statements)

References 207 publications

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“…The serum dosage of gonadotropins, TSH, and AMH concentrations by immunoassay is a routine practice in clinical applications in the diagnosis of ovarian conditions and for predicting the response to in vitro fertilization (IVF) therapy protocols. 17,18 Currently, the mass spectrometry platform is becoming a valid support to clinical laboratories, especially in metabolomics, as the sample manipulation is minimal and the analysis cost and time are enormously decreased using a targeted approach by LC-MRM/MS. The proteomics approach suffers from the digestion step, which lasts around 16 h for enzymatic hydrolysis.…”

Section: Resultsmentioning

confidence: 99%

Determination of Fertility Hormones and Adipokines by LC-MRM/MS Analysis

Illiano,

Pinto,

Mallardo

et al. 2024

ACS Omega

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Due to the increase in the rate of male and female infertility, assisted fertilization practices are currently adopted as valid support for couples unable to get pregnant. Analytical approaches for fertility hormone dosages are constantly being developed, following the technological progress of fertilization methods that have evolved for more than a century. Indeed, the analysis of fertility hormones in serum samples is a common clinical practice to check the fertility state, but absolute quantification of these hormones is a great challenge due to biological variability and low serum concentrations. Currently, ELISA (enzyme-linked immunosorbent assay) based methods are the most used analytical techniques to quantify hormones in blood in clinical settings. The current Article discusses the development of a liquid chromatography-tandem mass spectrometry method (LC-MS/MS) to monitor multiple fertility hormones of a protein nature in a single chromatographic run, i.e., LH (luteinizing hormone), FSH (follicle-stimulating hormone), TSH (thyroid-stimulating hormone), AMH (anti-Mullerian hormone), adiponectin, ghrelin, leptin, glucagon, and obestatin. Particular attention has been paid to the AMH hormone, whose ELISA-based quantification is known to be controversial due to the poor reproducibility between the various kits used. For AMH, the internal standard method was used for the quantitative determination to compare mass spectrometry data to the ELISA assays performed by an accredited analysis laboratory on a cohort of samples from women aged between 18 and 60 years. The ability to monitor multiple transitions by LC-MRM/MS ensured both high specificity and high selectivity, which is necessary for the quantification of protein and steroid hormones, besides improvements in data reproducibility and reduced analysis times and costs.

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Section: Resultsmentioning

confidence: 99%

Determination of Fertility Hormones and Adipokines by LC-MRM/MS Analysis

Illiano,

Pinto,

Mallardo

et al. 2024

ACS Omega

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“…The subject of contraceptives has been a subject of significant discourse for several decades since the promotion of contraception and family planning not only improves people’s general welfare and health but also adds to economic prosperity. The progressions in the field of nanotechnology have resulted in notable enhancements in contraceptive methodologies throughout the last several decades . The effectiveness of immuno-contraceptive vaccines using chitosan nanoparticles has been shown in the delivery of peptides and proteins, showcasing enhanced uptake by dendritic cells and extended presence in lymph nodes .…”

Section: Nanotech Advancements For Male and Female Contraceptionmentioning

confidence: 99%

“…The progressions in the field of nanotechnology have resulted in notable enhancements in contraceptive methodologies throughout the last several decades. 242 The effectiveness of immuno-contraceptive vaccines using chitosan nanoparticles has been shown in the delivery of peptides and proteins, showcasing enhanced uptake by dendritic cells and extended presence in lymph nodes. 243 Further investigation revealed that the use of disposable polymer-based polyethylene sebacate particles demonstrated efficacy in the administration of an HSA peptide-1 vaccination.…”

Section: Nanotech Advancements For Male and Female Contraceptionmentioning

confidence: 99%

Recent Advances in Nanotechnology-Based Drug Delivery Systems for the Diagnosis and Treatment of Reproductive Disorders

Acharya,

Behera,

Behera

et al. 2024

ACS Appl. Bio Mater.

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Over the past decade, nanotechnology has seen extensive integration into biomedical applications, playing a crucial role in biodetection, drug delivery, and diagnostic imaging. This is especially important in reproductive health care, which has become an emerging and significant area of research. Global concerns have intensified around disorders such as infertility, endometriosis, ectopic pregnancy, erectile dysfunction, benign prostate hyperplasia, sexually transmitted infections, and reproductive cancers. Nanotechnology presents promising solutions to address these concerns by introducing innovative tools and techniques, facilitating early detection, targeted drug delivery, and improved imaging capabilities. Through the utilization of nanoscale materials and devices, researchers can craft treatments that are not only more precise but also more effective, significantly enhancing outcomes in reproductive healthcare. Looking forward, the future of nanotechnology in reproductive medicine holds immense potential for reshaping diagnostics, personalized therapies, and fertility preservation. The utilization of nanotechnology-driven drug delivery systems is anticipated to elevate treatment effectiveness, minimize side effects, and offer patients therapies that are not only more precise but also more efficient. This review aims to delve into the various types, properties, and preparation techniques of nanocarriers specifically designed for drug delivery in the context of reproductive disorders, shedding light on the current landscape and potential future directions in this dynamic field.

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