State of the art in cerebral venous sinus thrombosis animal models

Bourrienne, Marie‐Charlotte; Gay, Juliette; Mazighi, Mikaël; Ajzenberg, Nadine

doi:10.1111/jth.15816

Cited by 8 publications

(3 citation statements)

References 75 publications

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“…Mouse models have been used extensively to elevate our understanding of the mechanisms of thrombopoiesis (platelet production) and multiplex functions of platelets under physiological and pathological conditions. These mouse models represent an integral approach that underpins platelet and thrombosis research (Bourrienne et al., 2022; Han et al., 2022; Palacios‐Acedo et al., 2020; Ware, 2022). Several excellent publications describe in great detail about the care and handling of laboratory mice (Donovan & Brown, 2013), as well as blood collection methods (Ahrens Kress et al., 2022; Aurbach et al., 2019; Parasuraman et al., 2010; Yang et al., 2019); however, these methods rely upon the use of skilled blood collection techniques such as retro‐orbital bleeding or cardiac puncture that requires anesthesia as well as the use of specialized pre‐coated anticoagulant tubes to avoid blood dilution errors.…”

Section: Introductionmentioning

confidence: 99%

An Optimized Method of Collecting Murine Peripheral Blood and Dilution Correction for Accurate Blood Cell Enumeration

Bhoopalan,

Gardiner,

Kaur

2023

Current Protocols

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Accurate measurement of whole blood counts from mice is an essential quantitative tool across the fields of vascular cell biology. In particular, the measurement of platelet counts can be challenging as the process relies upon good phlebotomy technique, the inclusion of a sufficient amount of the appropriate anticoagulant, and very often dilution of the sample to meet the sample volume requirements of an automated analyzer. To minimize sample dilution, blood collection tubes pre‐coated with the anticoagulant can be used; however, these are expensive and prone to blood clotting issues. Here, we describe a simple dilution correction method that accurately calculates blood‐to‐anticoagulant dilutions to generate appropriate volumes for automated blood cell analysis while minimizing blood clotting. We also discuss some simple steps that can be incorporated into blood collection methods to avoid artefacts during blood collection. Blood count data analysis involving volume correction and clot exclusion can significantly reduce variable blood cell count values among healthy untreated littermates. It also detects subtle changes in blood cell counts, mainly of platelets and RBCs in experimental settings, which can be masked in the absence of careful and precise volume correction. Blood count analysis with volume correction precisely determines mouse whole blood cell counts for investigators. The decreased variability in cell count values reduces the number of experimental animals required for meaningful analysis. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol: An optimized method of collecting murine peripheral blood and dilution correction for accurate blood cell enumeration

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Section: Introductionmentioning

confidence: 99%

An Optimized Method of Collecting Murine Peripheral Blood and Dilution Correction for Accurate Blood Cell Enumeration

Bhoopalan,

Gardiner,

Kaur

2023

Current Protocols

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“…These ndings further support the idea that the dural sinus is a crucial site for immune-blood-brain interactions where CNS antigens are exposed. APCs capture and present these antigens to circulating T cells, which subsequently become activated for immune surveillance of the CNS [11,12].…”

Section: Discussionmentioning

confidence: 99%

Giant cell arteritis combined with venous sinus thrombosis: A case report and systematic review

Li,

Zhang,

Wang

2023

Preprint

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Background Giant cell arteritis (GCA) is a low-incidence systemic vasculitis in Asian populations. It is considered a rare cause of cerebral venous sinus thrombosis (CVST). This case report presents a rare case of an Asian patient with GCA accompanied by CVST. Case Presentation: A 67-year-old man who had been experiencing varicose veins in the bilateral temporal region for four years and complete loss of vision in the right eye for four months sought medical attention in our emergency department due to loss of vision in the left eye for two weeks and fever for three days. On admission, this patient had a recurrent fever, and no source of infection was identified. Conventional anti-infective treatment was ineffective. Based on the clinical presentation, a diagnosis of GCA combined with CVST was made, and hormone therapy was administered. The patient was discharged with normal body temperature, clear consciousness, recovery of vision in both eyes, and improved clinical parameters. Conclusions This case report presents a distinct manifestation of both GCA and CVST. The coexistence of GCA and CVST is not solely attributed to increased blood viscosity, inflammation of the vascular wall, and abnormal coagulation. It also involves a complex immunological network. This case report may help clinicians make comprehensive clinical decisions in similar cases.

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“…CVST is associated with a wide range of clinical symptoms from isolated headaches (the most common symptom at presentation, reported in 90% of cases) to focal deficits, seizures, and mental status changes. 2 The variability and nonspecificity of the clinical course make the diagnosis and prognosis of CVST cases challenging. Moreover, according to recent research, 3 the incidence of CVST has increased more than previously estimated with the advent of modern diagnostic techniques, with an overall death and dependency rate of 15%.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of Poor Prognosis in Patients with Cerebral Venous Sinus Thrombosis: A Multicenter Retrospective Study

Zhang¹,

Liu²,

Qin³

et al. 2023

NDT

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Purpose The present study aimed to identify the characteristics, predictors, and imaging features of poor recovery in cases of cerebral venous sinus thrombosis (CVST). Patients and Methods A total of 290 consecutive adult patients with CVST were enrolled from January 2017 to December 2021 from five hospitals in Nanning, Guangxi. According to the modified Rankin scale (mRS) score at hospital discharge, the patients were classified into good prognosis (GP, mRS ≤2) groups and poor prognosis (PP, mRS>2) groups. Logistic regression was used to identify factors associated with clinical outcomes. Results Of the 290 patients, 35 were assigned to the PP group and 255 to the GP group. No significant difference in sex was observed between the two groups. Headache (76.21%) was the most frequent symptom of CVST, and local head and neck infection was the major comorbidity (26.21%). Approximately half of the patients (48.62%) had brain injury lesions <1 cm, and the most commonly affected sinus was the lateral sinus (81.03%). Less-common headaches (odds ratio [OR]: 2.769, p=0.046), altered mental status (OR: 0.122, p<0.001), hematologic disorder (OR: 0.191, p=0.045), and injury to multiple lobes (OR: 0.166, p=0.041) were associated with poor clinical outcomes. Conclusion Headache was the most common and protective manifestation of CVST, and disturbances in consciousness were an important indication of poor clinical prognosis. Patients with hematologic diseases also tended to have poor outcomes. No significant correlation was found between the number and location of venous sinus thromboses and clinical prognosis; however, intracranial injury involving multiple lobes was often associated with poor prognosis.

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State of the art in cerebral venous sinus thrombosis animal models

Cited by 8 publications

References 75 publications

An Optimized Method of Collecting Murine Peripheral Blood and Dilution Correction for Accurate Blood Cell Enumeration

An Optimized Method of Collecting Murine Peripheral Blood and Dilution Correction for Accurate Blood Cell Enumeration

Giant cell arteritis combined with venous sinus thrombosis: A case report and systematic review

Characteristics of Poor Prognosis in Patients with Cerebral Venous Sinus Thrombosis: A Multicenter Retrospective Study

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