State-of-the-art progress of switch fluorescence biosensors based on metal-organic frameworks and nucleic acids

Huo, Yapeng; Li, Sha; Gao, Zhixian; Ning, Baoan; Wang, Yu

doi:10.1007/s00604-021-04827-9

Cited by 31 publications

(12 citation statements)

References 108 publications

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“…44 In addition, MOFs are promising fluorescent quenching agents that can bind to biological recognition molecules and affect the switching states of fluorescence, as shown in Figure 3C. 41 Based on this, MOFs have been employed for sensitive and efficient quantitative analysis of various samples. 45 Upconversion nanomaterials are materials with anti-Stokes shift properties that can emit short-wavelength light (visible or ultraviolet) under the irradiation of long-wavelength light (near-infrared).…”

Section: Fluorescence-based Sensorsmentioning

confidence: 99%

Microfluidic preparation of optical sensors for biomedical applications

Wang

Yang

et al. 2023

Smart Medicine

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Optical biosensors are platforms that translate biological information into detectable optical signals, and have extensive applications in various fields due to their characteristics of high sensitivity, high specificity, dynamic sensing, etc. The development of optical sensing materials is an important part of optical sensors. In this review, we emphasize the role of microfluidic technology in the preparation of optical sensing materials and the application of the derived optical sensors in the biomedical field. We first present some common optical sensing mechanisms and the functional responsive materials involved. Then, we describe the preparation of these sensing materials by microfluidics. Afterward, we enumerate the biomedical applications of these optical materials as biosensors in disease diagnosis, drug evaluation, and organ‐on‐a‐chip. Finally, we discuss the challenges and prospects in this field.

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Section: Fluorescence-based Sensorsmentioning

confidence: 99%

Microfluidic preparation of optical sensors for biomedical applications

Wang

Yang

et al. 2023

Smart Medicine

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“…Metal–organic frameworks (MOFs) are crystalline nanomaterials made up of metal ions and organic ligands. − MOFs are widely used in a variety of fields, including separation, gas adsorption, energy storage, and catalysis, due to their huge specific areas, simple synthesis procedures, plentiful functional groups, enzyme-mimetic behavior, and chemical stability. , Because of these benefits, MOFs are particularly well-suited for the construction of biosensors with a wide range of applications, particularly in the biomedical domains. − Recently, researchers are interested in integrating nucleic acids with MOFs for biosensing. − Moreover, the enzyme-mimetic activity or fluorescence emission of some MOFs allows for using this entity in colorimetric or fluorescence assay. − To this end, rational selection of MOFs with enzyme-mimetic behavior and fluorescence emission gives the best route toward functionalization with a suitable aptamer.…”

Section: Introductionmentioning

confidence: 99%

Nanozyme and Stimulated Fluorescent Cu-Based Metal–Organic Frameworks (Cu-MOFs) Functionalized with Engineered Aptamers as a Molecular Recognition Element for Thrombin Detection in the Plasma of COVID-19 Patients

Ali

Omer

2022

ACS Omega

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An essential tool in the management and control of the COVID-19 pandemic is the development of a fast, selective, sensitive, and inexpensive COVID-19 biomarkers detection method. Herein, an ultrasensitive and labelfree biosensing strategy was described for the colorimetric and fluorimetric detection of thrombin. A dual-mode aptasensing method based on integrating engineered ssDNA with a stimulated fluorescent enzyme-mimetic copper-based metal−organic framework (Cu-MOF) as a molecular recognition element for thrombin was investigated. Cu-MOFs displayed stimulated fluorescence and enzyme-mimetic peroxidase activities that oxidize the chromogenic colorless substance TMB to blue-colored oxTMB. The thrombin-based aptamer (ssDNA) can be immobilized on the Cu-MOF surface to form a functionalized composite, ssDNA/MOF, and quench the stimulated fluorescence emission and the enzymatic activity of the Cu-MOF. Later, addition of thrombin recovers the fluorescence and enzymatic activity of the MOF. Thus, a turn-on colorimetry/fluorimetry aptasensing probe was designed for the detection of thrombin. Based on colorimetric assay, 350 pM was recorded as the lower limit of detection (LOD), while based on the fluorescence mode, 110 fM was recorded as the LOD (when S/N = 3). The label-free aptasensing probe was used successfully for the detection of thrombin in COVID-19 patients with satisfactory recoveries, 95−98%. Since the detection time of our aptasensor is relatively rapid (45 min) and due to the low-cost precursors and easy-to-operate characteristics, we believe that it has great potential to be used in point-of-care testing (POCT).

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“…Besides their unique optical properties, the high porosity and the abundant active sites of MOFs provide the spatial environment and multiple recognition sites and facilitate the adsorption of analyte in the pores, leading to the preconcentration of target and the enhancement of detection sensitivity. Recently, a few review papers have summarized the applications of MOFs-based materials in chemical/biological sensing [ 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. For example, Wu’s group and Wang’s group reviewed the recent progress in fluorescence biosensors based on DNA–MOF hybrids [ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, a few review papers have summarized the applications of MOFs-based materials in chemical/biological sensing [ 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. For example, Wu’s group and Wang’s group reviewed the recent progress in fluorescence biosensors based on DNA–MOF hybrids [ 36 , 37 ]. Jia’s group discussed the post-synthetic modification methods for MOFs-based fluorescent sensors [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

An Overview of the Design of Metal-Organic Frameworks-Based Fluorescent Chemosensors and Biosensors

et al. 2022

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Taking advantage of high porosity, large surface area, tunable nanostructures and ease of functionalization, metal-organic frameworks (MOFs) have been popularly applied in different fields, including adsorption and separation, heterogeneous catalysis, drug delivery, light harvesting, and chemical/biological sensing. The abundant active sites for specific recognition and adjustable optical and electrical characteristics allow for the design of various sensing platforms with MOFs as promising candidates. In this review, we systematically introduce the recent advancements of MOFs-based fluorescent chemosensors and biosensors, mainly focusing on the sensing mechanisms and analytes, including inorganic ions, small organic molecules and biomarkers (e.g., small biomolecules, nucleic acids, proteins, enzymes, and tumor cells). This review may provide valuable references for the development of novel MOFs-based sensing platforms to meet the requirements of environment monitoring and clinical diagnosis.

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State-of-the-art progress of switch fluorescence biosensors based on metal-organic frameworks and nucleic acids

Cited by 31 publications

References 108 publications

Microfluidic preparation of optical sensors for biomedical applications

Microfluidic preparation of optical sensors for biomedical applications

Nanozyme and Stimulated Fluorescent Cu-Based Metal–Organic Frameworks (Cu-MOFs) Functionalized with Engineered Aptamers as a Molecular Recognition Element for Thrombin Detection in the Plasma of COVID-19 Patients

An Overview of the Design of Metal-Organic Frameworks-Based Fluorescent Chemosensors and Biosensors

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