The end restraint strength of the reinforced concrete (RC) beams under the fire conditions may greatly influence the inner force redistribution and the structural behavior of the beams, even the stability of the whole structure. The structural fire behavior of two full‐scale frame beams with different end restraint strength under load were studied based on experiment and simulation. The temperature field, reinforcement strain, beam deflection, end axial displacement, and rotation angle of the structure during the whole heating and cooling process were measured. The influence of end restraint strength on the structural behavior was analyzed. The results show that the vertical displacement, axial force, end bending moment, and angular displacement first increase rapidly, then decrease gradually and become stable in the whole process; the lateral displacement increases first and then stabilizes; the change of axial and rotational deformations appeared to be non‐proportional with the corresponding restraining forces, reflecting that the axial and the rotational restraint strength at the ends of the beams decreased with the rise of temperature. The restraining strength of beams that submitted to fire globally decreased faster, and leading to a severe fire response and an earlier failure of the structure. The analysis results and the conclusions will supply scientific basis for the fire‐resistance design and safety evaluation on complex structures.