State-of-the-arts techniques and current evolving approaches in the separation and detection of circulating tumor cell

He, Sitian; Wei, Jinlan; Ding, Lei; Yang, Xiaonan; Wu, Yongjun

doi:10.1016/j.talanta.2021.123024

Cited by 16 publications

(10 citation statements)

References 142 publications

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“…35 Further refinement of this nanopore assay using MUC1 as a singlemolecule biomarker reduced the LOD of BrCa CTCs to 2 cells/mL. 36 Following these indicative examples of CTC enrichment strategies, the reader is referred to recent review articles for a detailed description of additional classic techniques (e.g., density-based gradient centrifugation, Figure 1), 37 in vivo capture and the diagnostic leukapheresis (DLA), 32 or functional approaches (e.g., EPISPOT-Epithelial ImmunoSPOT which evolved into EPIDROP-EPIspot in a DROP) for CTC detection, 38 but also lately developed magnetic nanoparticles 39 and microfluidic chips. 32 Comparative tables of different techniques with their corresponding performance metrics (efficiency, purity, recovery, viability, throughput) can also be found in the literature.…”

Section: Advances In Ctc Isolation and Sorting In Bulk And At The Sin...mentioning

confidence: 99%

Circulating Tumor Cells in Cancer Diagnostics and Prognostics by Single-Molecule and Single-Cell Characterization

Chowdhury¹,

Cressiot

Parisi

et al. 2023

ACS Sens.

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Circulating tumor cells (CTCs) represent an interesting source of biomarkers for diagnosis, prognosis, and the prediction of cancer recurrence, yet while they are extensively studied in oncobiology research, their diagnostic utility has not yet been demonstrated and validated. Their scarcity in human biological fluids impedes the identification of dangerous CTC subpopulations that may promote metastatic dissemination. In this Perspective, we discuss promising techniques that could be used for the identification of these metastatic cells. We first describe methods for isolating patient-derived CTCs and then the use of 3D biomimetic matrixes in their amplification and analysis, followed by methods for further CTC analyses at the single-cell and single-molecule levels. Finally, we discuss how the elucidation of mechanical and morphological properties using techniques such as atomic force microscopy and molecular biomarker identification using nanopore-based detection could be combined in the future to provide patients and their healthcare providers with a more accurate diagnosis.

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Section: Advances In Ctc Isolation and Sorting In Bulk And At The Sin...mentioning

confidence: 99%

Circulating Tumor Cells in Cancer Diagnostics and Prognostics by Single-Molecule and Single-Cell Characterization

Chowdhury¹,

Cressiot

Parisi

et al. 2023

ACS Sens.

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“…Cancer cells are excreted from the primary tumor and then they enter the circulatory system as CTCs. A small portion of it may develop into metastatic cancer under appropriate microenvironmental conditions [ 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the detection of CTC from the blood of oncologic patients by a so-called liquid biopsy [ 3 ] is a non-invasive way for the identification of lesions and may thus form a clinical basis for the development of a targeted therapy [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…The main problems of CTC detection are the rarity and heterogeneity of their occurrence in the blood, leading to higher costs in the production of adequately effective separators with low interception performance [ 2 ]. Therefore, numerous and intensive studies on methods for effective enrichment of CTC [ 12 , 13 , 14 , 15 , 16 ], isolation, and identification [ 17 , 18 , 19 , 20 ] have been carried out.…”

Section: Introductionmentioning

confidence: 99%

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Studies on the Effect of Graphene Oxide Deposited on Gold and Nickel Microsieves on Prostate Cancer Cells DU 145

Nasiłowska

Bogdanowicz

Kasprzycka

et al. 2022

IJMS

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This work shows the effect of graphene oxide deposition on microsieves’ surfaces of gold and nickel foils, on DU 145 tumor cells of the prostate gland. The sieves were made by a laser ablation process. The graphene oxide (GO) deposition process was characterized by the complete covering of the inner edges of the microholes and the flat surface between the holes with GO. Electron microscanning studies have shown that due to the deposition method applied, graphene oxide flakes line the interior of the microholes, reducing the unevenness of the downstream surfaces during the laser ablation process. The presence of graphene oxide was confirmed by Fourier infrared spectroscopy. During the screening (sieving) process, the microsieves were placed in a sieve column. Gold foil is proven to be a very good material for the screening of cancer cells, but even more so after screening as a substrate for re-culture of the DU 145. This allows a potential recovery of the cells and the development of a targeted therapy. The sieved cells were successfully grown on the microsieves used in the experiment. Graphene oxide remaining on the surface of the nickel sieve has been observed to increase the sieving effect. Although graphene oxide improved separation efficiency by 9.7%, the nickel substrate is not suitable for re-culturing of the Du 145 cells and the development of a targeted therapy compared to the gold one.

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“…Therefore, there is a crucial requirement to develop an innovative point-of-care device that is user-friendly, efficient, low cost and has high sensitivity [ 11 ]. Electrochemical sensors for tumor cell evaluation, compared with traditional tissue biopsy, which is considered the “gold standard”, can be used for significantly advanced strategies including the analysis of rare cells or molecules in blood samples [ 12 , 13 , 14 ]. For tumor cell detection, electrochemical biosensors offer a remarkable solution for cancer monitoring as a result of their high sensitivity, good performance and rapid real-time quantitative information.…”

Section: Introductionmentioning

confidence: 99%

Vertical Graphene-Based Biosensor for Tumor Cell Dielectric Signature Evaluation

et al. 2022

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The selective and rapid detection of tumor cells is of critical consequence for the theragnostic field of tumorigenesis; conventional methods, such as histopathological diagnostic methods, often require a long analysis time, excessive analytical costs, complex operations, qualified personnel and deliver many false-positive results. We are considering a new approach of an electrochemical biosensor based on graphene, which is evidenced to be a revolutionary nanomaterial enabling the specific and selective capture of tumor cells. In this paper, we report a biosensor fabricated by growing vertically aligned graphene nanosheets on the conductive surface of interdigitated electrodes which is functionalized with anti-EpCAM antibodies. The dielectric signature of the three types of tumor cells is determined by correlating the values from the Nyquist and Bode diagram: charge transfer resistance, electrical double layer capacity, Debye length, characteristic relaxation times of mobile charges, diffusion/adsorption coefficients, and variation in the electrical permittivity complex and of the phase shift with frequency. These characteristics are strongly dependent on the type of membrane molecules and the electromagnetic resonance frequency. We were able to use the fabricated sensor to differentiate between three types of tumor cell lines, HT-29, SW403 and MCF-7, by dielectric signature. The proposed evaluation method showed the permittivity at 1 MHz to be 3.63 nF for SW403 cells, 4.97 nF for HT 29 cells and 6.9 nF for MCF-7 cells.

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State-of-the-arts techniques and current evolving approaches in the separation and detection of circulating tumor cell

Cited by 16 publications

References 142 publications

Circulating Tumor Cells in Cancer Diagnostics and Prognostics by Single-Molecule and Single-Cell Characterization

Circulating Tumor Cells in Cancer Diagnostics and Prognostics by Single-Molecule and Single-Cell Characterization

Studies on the Effect of Graphene Oxide Deposited on Gold and Nickel Microsieves on Prostate Cancer Cells DU 145

Vertical Graphene-Based Biosensor for Tumor Cell Dielectric Signature Evaluation

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