Statherin and alpha-amylase levels in saliva from patients with gingivitis and periodontitis

Parlak, Hanife Merva; Büber, Esra; Gur, Ali Tugrul; Karabulut, Erdem; Akalın, Ferda Alev

doi:10.1016/j.archoralbio.2022.105574

Cited by 7 publications

(3 citation statements)

References 42 publications

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“…These findings are consistent with a study conducted by Parlak et al, which reported a significant correlation between the age of participants and the activity of salivary enzymes. 14 Additionally, the study showed that there was no significant correlation between alpha amylase levels and the duration of smoking. These findings are inconsistent with the previous studies.…”

Section: Discussionmentioning

confidence: 92%

“…This result was consistent with the research conducted by Parlak et al and Rashid et al, which demonstrated that periodontitis can lead to an elevated production of salivary proteins, such as mucin and amylase. 14,15 Patients with moderate to severe periodontitis had higher concentrations of these two forms of proteins. According to a study by Papacosta, salivary alpha-amylase serves as the initial defense mechanism, which aligns with the observed increase in alpha-amylase levels in periodontitis.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of salivary alpha amylase activity in smokers with periodontitis, Khartoum state, 2023

Allah,

Ahmed

2023

Eur J Clin Exp Med

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Introduction and aim. Smoking widely affect oral health, including its role in the development of periodontitis. Saliva contains an antioxidant system and various enzymes. The study was designed to evaluate the activity of salivary alpha amylase among individuals who smoke and have periodontitis. Material and methods. A total of 100 participants were included, with 50 cases (cigarette smokers with periodontitis) and the remaining 50 nonsmokers with healthy periodontium as the control group. Saliva samples were collected to measure salivary alpha amylase activity. Results. Smokers with periodontitis had significantly higher levels of salivary alpha amylase compared to the control group (177.96±14.5 vs 94.04±19.6 IU/mL, p<0.001). Additionally, there was a weak negative correlation between the level of alpha amylase and the age of the patients (p=0.01, r=0.376). However, no correlation was found between the level of alpha amylase and the duration of smoking (p=0.584, r=0.079). Conclusion. There is a significant increase in salivary alpha amylase levels among smokers with periodontitis. No correlation was found between age and salivary alpha amylase levels. However, a weak positive correlation was observed between the duration of smoking and salivary alpha amylase activity.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Evaluation of salivary alpha amylase activity in smokers with periodontitis, Khartoum state, 2023

Allah,

Ahmed

2023

Eur J Clin Exp Med

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“…For instance, salivary α-amylase levels were significantly higher in youths with anxiety disorders compared to healthy controls [59]. In the context of periodontitis, it has been revealed that patients with the disease exhibit significantly higher levels of salivary α-amylase activity in UWS when compared to healthy controls [60,61]. Additionally, a positive correlation has been established between salivary α-amylase activity and the number of teeth affected by periodontal disease with a probing pocket depth of 5 mm or greater [62].…”

Section: Discussionmentioning

confidence: 99%

Mass Spectrometry-Based Proteomics for Discovering Salivary Biomarkers in Periodontitis: A Systematic Review

Hu,

Leung

2023

IJMS

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Periodontitis is one of the primary causes of tooth loss, and is also related to various systemic diseases. Early detection of this condition is crucial when it comes to preventing further oral damage and the associated health complications. This study offers a systematic review of the literature published up to April 2023, and aims to clearly explain the role of proteomics in identifying salivary biomarkers for periodontitis. Comprehensive searches were conducted on PubMed and Web of Science to shortlist pertinent studies. The inclusion criterion was those that reported on mass spectrometry-driven proteomic analyses of saliva samples from periodontitis cohorts, while those on gingivitis or other oral diseases were excluded. An assessment for risk of bias was carried out using the Newcastle–Ottawa Scale and Quality Assessment of Diagnostic Accuracy Studies or the NIH quality assessment tool, and a meta-analysis was performed for replicable candidate biomarkers, i.e., consistently reported candidate biomarkers (in specific saliva samples, and periodontitis subgroups, reported in ≥2 independent cohorts/reports) were identified. A Gene Ontology enrichment analysis was conducted using the Database for Annotation, Visualization, and Integrated Discovery bioinformatics resources, which consistently expressed candidate biomarkers, to explore the predominant pathway wherein salivary biomarkers consistently manifested. Of the 15 studies included, 13 were case–control studies targeting diagnostic biomarkers for periodontitis participants (periodontally healthy/diseased, n = 342/432), while two focused on biomarkers responsive to periodontal treatment (n = 26 participants). The case–control studies were considered to have a low risk of bias, while the periodontitis treatment studies were deemed fair. Summary estimate and confidence/credible interval, etc. determination for the identified putative salivary biomarkers could not be ascertained due to the low number of studies in each case. The results from the included case–control studies identified nine consistently expressed candidate biomarkers (from nine studies with 230/297 periodontally healthy/diseased participants): (i) those that were upregulated: alpha-amylase, serum albumin, complement C3, neutrophil defensin, profilin-1, and S100-P; and (ii) those that were downregulated: carbonic anhydrase 6, immunoglobulin J chain, and lactoferrin. All putative biomarkers exhibited consistent regulation patterns. The implications of the current putative marker proteins identified were reviewed, with a focus on their potential roles in periodontitis diagnosis and pathogenesis, and as putative therapeutic targets. Although in its early stages, mass spectrometry-based salivary periodontal disease biomarker proteomics detection appeared promising. More mass spectrometry-based proteomics studies, with or without the aid of already available clinical biochemical approaches, are warranted to aid the discovery, identification, and validation of periodontal health/disease indicator molecule(s). Protocol registration number: CRD42023447722; supported by RD-02-202410 and GRF17119917.

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A New Strategy for Measuring Salivary Alpha‐Amylase Activity Using Glucometer

Abdi,

Gorgani‐Firuzjaee,

Behroozi

et al. 2024

Starch Stärke

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Alpha‐amylase is one of the most important enzymes in the saliva. It is an important biomarker for many health conditions. Saliva sampling is a non‐invasive method and is the preferred choice for sampling at point of the care and home testing. Measurement of salivary alpha‐amylase activity as a rapid test using test strips with Flavin‐dependent glucose dehydrogenases (FAD‐GDH) as glucose is the aim of the current research. FAD‐GDH is an oxygen‐independent enzyme and has a narrow substrate specificity. For this goal, starches from various sources are tested as a substrate for alpha‐amylase and chose MS‐101 starch as the best substrate, between six different starch types. Under optimal assay conditions, when hydrolyzed starch (in 20 min, 37 °C, pH = 7) reacts with FAD‐GDH enzymes that exist in test strips for 5 s glucometer can show the amount of glucose produced in samples from patient samples. This glucose value is directly correlated with the salivary alpha‐amylase activity (p‐value <0.01).

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Statherin and alpha-amylase levels in saliva from patients with gingivitis and periodontitis

Cited by 7 publications

References 42 publications

Evaluation of salivary alpha amylase activity in smokers with periodontitis, Khartoum state, 2023

Evaluation of salivary alpha amylase activity in smokers with periodontitis, Khartoum state, 2023

Mass Spectrometry-Based Proteomics for Discovering Salivary Biomarkers in Periodontitis: A Systematic Review

A New Strategy for Measuring Salivary Alpha‐Amylase Activity Using Glucometer

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