Static magnetic field controls cell cycle in cultured human glioblastoma cells

Kim, Seung Chan; Im, Wooseok; Shim, Jay Yong; Kim, Seung-Ki; Kim, Beom Jin

doi:10.1007/s10616-016-9973-2

Cited by 8 publications

(4 citation statements)

References 32 publications

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“…Our results demonstrated that SH-SY5Y cells exposed to SMS for 24 h show a decrease in cell viability immediately after the exposure (24 h). A previous study using glioblastoma cells submitted to SMS for 24 h corroborates these findings regarding cell viability (15). This effect on cell viability, however, was not long-lasting, since 24 h after exposure treated groups were not different from the control group.…”

Section: Discussionsupporting

confidence: 80%

“…Potential uses and effects of magnetic stimulation over cellular processes have been described, particularly using static magnetic stimulation (SMS). Apart from physiological and homeostatic events, such as wound healing (14), SMS has shown effects in cancer models of glioblastoma (15), adenocarcinoma (16) and leukemia (17), where it has been shown to control the cell cycle, reduce drug resistance to cisplatin and enhance natural killer cell cytotoxicity against tumor cells, respectively. SMS, unlike repetitive transcranial magnetic stimulation (rTMS) -in which changes in the magnetic field create an electric current through electromagnetic induction -does not induce electric currents; however, it has been shown to influence a variety of biological systems (18).…”

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confidence: 99%

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Static Magnetic Stimulation Induces Cell-type Specific Alterations in the Viability of SH-SY5Y Neuroblastoma Cell Line

et al. 2020

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Background/Aim: Magnetic stimulation is used in the treatment of a diversity of diseases, but a complete understanding of the underlying mechanisms of action requires further investigation. We examined the effect of static magnetic stimulation (SMS) in different cell lines. Materials and Methods: A culture plate holder with attached NeFeB magnets was developed. Different magnetic field intensities and periods were tested in tumoral and nontumoral cell lines. To verify the cellular responses to SMS, cell viability, cell death, cell cycle and BDNF expression were evaluated. Results: Exposure of SH-SY5Y cells to SMS for 24 hours led to a decrease in cell viability. Analysis 24 h after stimulation revealed a decrease in apoptotic and double-positive cells, associated with an increase in the number of necrotic cells. Conclusion: The effects of SMS on cell viability are cell type-specific, inducing a decrease in cell viability in SH-SY5Y cells. This suggests that SMS may be a potential tool in the treatment of neuronal tumors.Over the years, several electrophysiological studies have expanded the understanding of normal brain activity and its pathological conditions. Technological advances have been an important part of the improvement of therapies and research in several areas such as neurology, psychology, and psychiatry (1). Brain stimulation is a tool for modulating brain function, allowing the association of activity patterns and cognitive function to establish cause-consequence relations (2). Brain stimulation techniques are usually divided into two different types: invasive and non-invasive (NIBS) techniques. Whereas invasive techniques involve greater risk for patients, as demonstrated by studies that compare the impact of different procedures (3, 4), noninvasive techniques have shown favorable results together with lower risks (3, 5). Indeed, NIBS's application has been described in different scenarios: 1. Cognitive improvement on depression (6, 7); 2. Improvement in post-stroke recovery (8); 3. Improvement of the memory and the quality of life of patients with Alzheimer's disease (9, 10); 4. Relief of 5151

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Section: Discussionsupporting

confidence: 80%

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confidence: 99%

Static Magnetic Stimulation Induces Cell-type Specific Alterations in the Viability of SH-SY5Y Neuroblastoma Cell Line

et al. 2020

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“…A concomitant rearrangement of the actin cytoskeleton was additionally reported [149]. This raises the general question of to which extent cells can respond to forces generated from static magnetic fields [150][151][152]. In the context of dentistry, it needs to be considered that ferromagnetic compounds can sometimes be found as part of a prosthetic material [153,154] within overdentures in the oral cavity of patients.…”

Section: Mechanotransduction To the Core: Yap/taz In The Periodontiummentioning

confidence: 98%

From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues

et al. 2021

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Among oral tissues, the periodontium is permanently subjected to mechanical forces resulting from chewing, mastication, or orthodontic appliances. Molecularly, these movements induce a series of subsequent signaling processes, which are embedded in the biological concept of cellular mechanotransduction (MT). Cell and tissue structures, ranging from the extracellular matrix (ECM) to the plasma membrane, the cytosol and the nucleus, are involved in MT. Dysregulation of the diverse, fine-tuned interaction of molecular players responsible for transmitting biophysical environmental information into the cell’s inner milieu can lead to and promote serious diseases, such as periodontitis or oral squamous cell carcinoma (OSCC). Therefore, periodontal integrity and regeneration is highly dependent on the proper integration and regulation of mechanobiological signals in the context of cell behavior. Recent experimental findings have increased the understanding of classical cellular mechanosensing mechanisms by both integrating exogenic factors such as bacterial gingipain proteases and newly discovered cell-inherent functions of mechanoresponsive co-transcriptional regulators such as the Yes-associated protein 1 (YAP1) or the nuclear cytoskeleton. Regarding periodontal MT research, this review offers insights into the current trends and open aspects. Concerning oral regenerative medicine or weakening of periodontal tissue diseases, perspectives on future applications of mechanobiological principles are discussed.

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“…CDK1-cyclin B, also known as cell division control protein kinase 2-cyclin B (cdc2-cyclin B) functions at the G2/M phase of the cell cycle, to accelerate cell mitosis ( 60 ). SMFs (200 ± 60 mT, 48 ± 4 h) induced human malignant glioblastomata, such as U87 and U251, to arrest the G2/M phase by downregulating the expressions of cyclin B1 and CDK1 ( 61 ). The p53 protein is a critical participant in the signal transduction pathway which mediated apoptosis and G1 cell cycle arrest in mammalian cells ( 62 ).…”

Section: The Effects Of Mfs On Cell Proliferation Cell Cycle Arrestmentioning

confidence: 99%

Progressive Study on the Non-thermal Effects of Magnetic Field Therapy in Oncology

Wang

et al. 2021

Front. Oncol.

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Cancer is one of the most common causes of death worldwide. Although the existing therapies have made great progress and significantly improved the prognosis of patients, it is undeniable that these treatment measures still cause some serious side effects. In this context, a new treatment method is needed to address these shortcomings. In recent years, the magnetic fields have been proposed as a novel treatment method with the advantages of less side effects, high efficiency, wide applications, and low costs without forming scars. Previous studies reported that static magnetic fields (SMFs) and low-frequency magnetic fields (LF-MFs, frequency below 300 Hz) exert anti-tumor function, independent of thermal effects. Magnetic fields (MFs) could inhibit cell growth and proliferation; induce cell cycle arrest, apoptosis, autophagy, and differentiation; regulate the immune system; and suppress angiogenesis and metastasis via various signaling pathways. In addition, they are effective in combination therapies: MFs not only promote the absorption of chemotherapy drugs by producing small holes on the surface of cell membrane but also enhance the inhibitory effects by regulating apoptosis and cell cycle related proteins. At present, MFs can be used as drug delivery systems to target magnetic nanoparticles (MNPs) to tumors. This review aims to summarize and analyze the current knowledge of the pre-clinical studies of anti-tumor effects and their underlying mechanisms and discuss the prospects of the application of MF therapy in cancer prevention and treatment.

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Static magnetic field controls cell cycle in cultured human glioblastoma cells

Cited by 8 publications

References 32 publications

Static Magnetic Stimulation Induces Cell-type Specific Alterations in the Viability of SH-SY5Y Neuroblastoma Cell Line

Static Magnetic Stimulation Induces Cell-type Specific Alterations in the Viability of SH-SY5Y Neuroblastoma Cell Line

From the Matrix to the Nucleus and Back: Mechanobiology in the Light of Health, Pathologies, and Regeneration of Oral Periodontal Tissues

Progressive Study on the Non-thermal Effects of Magnetic Field Therapy in Oncology

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