Static magnetic fields accelerate osteogenesis by regulating FLRT/BMP pathway

Li, Weihao; Zhao, Shurong; He, Wei; Zhang, Ming; Li, Song; Xu, Yeqiong

doi:10.1016/j.bbrc.2020.04.090

Cited by 14 publications

(12 citation statements)

References 36 publications

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“…Uniform dispersion in the scaffold may show more contact surface area, thus providing more attachment sites for cell attachment [59]. However, the Fe3O4 nanoparticles are In addition, the magnetic field can also activate various signal pathways of the cell, and collaboratively mediate the signal communication between them, such as the classic mitogen-activated protein kinase [55][56][57] and BMP signal pathway [17,58], thereby promoting the expression of growth factors, improving the activity of runt-related transcription factor 2 and ALP, accelerating the growth and differentiation of osteoblasts, and promoting bone repair finally. Of course, the micro magnetic force generated in the microenvironment of the magnetic scaffold can provide continuous dynamic mechanical stimulation to MG63 cells, which can also improve the cells adhere and migrate.…”

Section: Cell Responsesmentioning

confidence: 99%

Micro Magnetic Field Produced by Fe3O4 Nanoparticles in Bone Scaffold for Enhancing Cellular Activity

et al. 2020

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The low cellular activity of poly-l-lactic acid (PLLA) limits its application in bone scaffold, although PLLA has advantages in terms of good biocompatibility and easy processing. In this study, superparamagnetic Fe3O4 nanoparticles were incorporated into the PLLA bone scaffold prepared by selective laser sintering (SLS) for continuously and steadily enhancing cellular activity. In the scaffold, each Fe3O4 nanoparticle was a single magnetic domain without a domain wall, providing a micro-magnetic source to generate a tiny magnetic field, thereby continuously and steadily generating magnetic stimulation to cells. The results showed that the magnetic scaffold exhibited superparamagnetism and its saturation magnetization reached a maximum value of 6.1 emu/g. It promoted the attachment, diffusion, and interaction of MG63 cells, and increased the activity of alkaline phosphatase, thus promoting the cell proliferation and differentiation. Meanwhile, the scaffold with 7% Fe3O4 presented increased compressive strength, modulus, and Vickers hardness by 63.4%, 78.9%, and 19.1% compared with the PLLA scaffold, respectively, due to the addition of Fe3O4 nanoparticles, which act as a nanoscale reinforcement in the polymer matrix. All these positive results suggested that the PLLA/Fe3O4 scaffold with good magnetic properties is of great potential for bone tissue engineering applications.

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Section: Cell Responsesmentioning

confidence: 99%

Micro Magnetic Field Produced by Fe3O4 Nanoparticles in Bone Scaffold for Enhancing Cellular Activity

et al. 2020

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“…The effects of magnetic fields influence biological activities [ 31 ], not only in osteoporosis [ 32 ] and orthodontics [ 33 ] but also with an active role in cartilage tissue engineering [ 34 ]. Related studies have provided evidence that moderate-intensity SMF can effectively promote chondrogenic differentiation of BMSCs [ 10 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…Related studies have provided evidence that moderate-intensity SMF can effectively promote chondrogenic differentiation of BMSCs [ 10 , 25 ]. Our earlier study tested different SMF strengths by exposing MCCs to 160, 280, or 360 mT and found that the proliferation and chondrogenesis-promoting effects of 280 mT SMF were the most pronounced [ 34 ]. In recent years, many studies have attempted to combine SMF with other elements [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Static Magnetic Fields Enhance the Chondrogenesis of Mandibular Bone Marrow Mesenchymal Stem Cells in Coculture Systems

Zhang

et al. 2021

BioMed Research International

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Objectives. Combining the advantages of static magnetic fields (SMF) and coculture systems, we investigated the effect of moderate-intensity SMF on the chondrogenesis and proliferation of mandibular bone marrow mesenchymal stem cells (MBMSCs) in the MBMSC/mandibular condylar chondrocyte (MCC) coculture system. The main aim of the present study was to provide an experimental basis for obtaining better cartilage tissue engineering seed cells for the effective repair of condylar cartilage defects in clinical practice. Methods. MBMSCs and MCCs were isolated from SD (Sprague Dawley) rats. Flow cytometry, three-lineage differentiation, colony-forming assays, immunocytochemistry, and toluidine blue staining were used for the identification of MBMSCs and MCCs. MBMSCs and MCCs were seeded into the lower and upper Transwell chambers, respectively, at a ratio of 1 : 2, and exposed to a 280 mT SMF. MBMSCs were harvested after 3, 7, or 14 days for analysis. CCK-8 was used to detect cell proliferation, Alcian blue staining was utilized to evaluate glycosaminoglycan (GAG), and western blotting and real-time quantitative polymerase chain reaction (RT-qPCR) detected protein and gene expression levels of SOX9, Col2A1 (Collagen Type II Alpha 1), and Aggrecan (ACAN). Results. The proliferation of MBMSCs was significantly enhanced in the experimental group with MBMSCs cocultured with MCCs under SMF stimulation relative to controls ( P < 0.05 ). GAG content was increased, and SOX9, Col2A1, and ACAN were also increased at the mRNA and protein levels ( P < 0.05 ). Conclusions. Moderate-intensity SMF improved the chondrogenesis and proliferation of MBMSCs in the coculture system, and it might be a promising approach to repair condylar cartilage defects in the clinical setting.

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“…Wang et al reported that SPIOs may induce MSC differentiation into osteoblasts via MAPK signaling pathway (18). SMF also has been shown to regulate the osteogenic differentiation of MSCs through the fibronectin leucine-rich transmembrane protein (FLRT)/bone morphogenetic protein (BMP) signaling pathway (21). SMF combine with SPIOs for MSCs transplanting has also been reported (19).…”

Section: Global Gene Expression Profile Of Spios and Smf-treated Nscsmentioning

confidence: 99%

Effects of superparamagnetic iron oxide nanoparticles and static magnetic fields on neural stem cell differentiation by transcriptomic techniques

Li¹,

Ma²,

Tang³

2022

STEMedicine

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Neural stem cells (NSCs)-based cell therapy provides promising treatment for the neurodegenerative diseases. The success of stem cell therapy relies on the efficient differentiation of transplanted stem cells into functional cells. Therefore, directed differentiation of NSCs into neurons is essential for its application in the neurodegenerative diseases. In recent years, magnetic fields and superparamagnetic iron oxide nanoparticles (SPIOs) have shown potential in the regulation of stem cell behaviors. Here, we investigated the regulatory effects of static magnetic fields (SMFs) and the combination with SPIO on NSC differentiation by transcriptome sequencing analysis techniques. Our results found that SPIOs caused more differentially expressed genes than SMF alone. Interestingly, the number of differentially expressed genes induced by the combination was less than that of SPIO alone, which may imply that the regulation is not a simple superposition effect. Our findings provide experimental evidence for the regulation of SMF and SPIO on NSC differentiation at the transcriptomic level.

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Static magnetic fields accelerate osteogenesis by regulating FLRT/BMP pathway

Cited by 14 publications

References 36 publications

Micro Magnetic Field Produced by Fe3O4 Nanoparticles in Bone Scaffold for Enhancing Cellular Activity

Micro Magnetic Field Produced by Fe3O4 Nanoparticles in Bone Scaffold for Enhancing Cellular Activity

Static Magnetic Fields Enhance the Chondrogenesis of Mandibular Bone Marrow Mesenchymal Stem Cells in Coculture Systems

Effects of superparamagnetic iron oxide nanoparticles and static magnetic fields on neural stem cell differentiation by transcriptomic techniques

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