Static magnetic fields generated by a 0.5T MRI unit affects in vitro expression of activation markers and interleukin release in human peripheral blood mononuclear cells (PBMC)

Salerno, Sergio; Casto, Antonio Lo; Caccamo, Nadia; D’Anna, Claudia; Maria, Muhtarova; Lagalla, Roberto; Scola, Letizia; Cardinale, A

doi:10.1080/095530099140384

Cited by 21 publications

(12 citation statements)

References 8 publications

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“…Salerno et al (1999) showed that exposure to a static magnetic field from a commercially available 0.5 T MRI unit induced modifications in the release of some interleukins in peripheral blood mononuclear cells. Lin et al (2008a) demonstrated that long-term SMF stimulation inhibits LPSinduced cytotoxicity through increased IL-6 production by fibroblasts, the response of which is similar to endotoxin tolerance.…”

Section: Introductionmentioning

confidence: 99%

A static magnetic field attenuates lipopolysaccharide-induced neuro-inflammatory response via IL-6-mediated pathway

Shen

Huang²,

Yang³

et al. 2013

Electromagnetic Biology and Medicine

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An effective method for controlling brain damage and neurodegeneration caused by inflammation remains elusive. Down-expression of the lipopolysaccharide (LPS)-induced inflammatory cytokines resulting in endotoxin tolerance is reported as an alternative anti-infection treatment. Nonetheless, because the dosage and action site are hard to control, endotoxin tolerance caused by low-dose LPS injection in brain tissue may induce side effects. The aim of this study was to test the hypothesis that static magnetic fields (SMF) stimulate endotoxin tolerance in brain tissue. In this study, survival rate and pathological changes in brain tissues of LPS-challenged mice were examined with and without SMF treatment. In addition, the effects of SMF exposure on growth rate and cytokine expression of LPS-challenged BV-2 microglia cells were monitored. Our results showed that SMF pre-exposure had positive effects on the survival rate and histological outcomes of LPS-treated mice. Furthermore, SMF exposure significantly decreased IL-6 expression in BV-2 cells (p < 0.05) by a phenomenon similar to endotoxin tolerance. We suggest that SMF has potential as an alternative simulation source for controlling LPS-induced excess neuro-inflammatory response.

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Section: Introductionmentioning

confidence: 99%

A static magnetic field attenuates lipopolysaccharide-induced neuro-inflammatory response via IL-6-mediated pathway

Shen

Huang²,

Yang³

et al. 2013

Electromagnetic Biology and Medicine

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“…In particular, the production of pro-inflammatory cytokines interleukin IL-6 and tumor necrosis factor TNF-α, and that of the anti-inflammatory cytokine IL-10 was studied in the work of Salerno et al [13] in vitro on human peripheral blood mononuclear cells (PBMC) under 0.5 T SMF continuous exposure for 24 h. The authors did not find any significant changes in the release of any cytokines. Aldinucci et al [14] published similar conclusions of a similar study concerning pro-inflammatory cytokines IL-6 and TNF-α on identical subject cells as Salerno et al [13], but the magnetic induction was continuous 4.75 T for 1 h. IL-8 was monitored by Sontag [15] in his in vitro study on human promyelocite cells (HL-60) exposed to 0–1.2 mT for a time period of 15 min.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Analysis of the Anti-Inflammatory Effect of Inhomogeneous Static Magnetic Field-Exposure on Human Macrophages and Lymphocytes

Vergallo

Szamosvölgyi²,

Tenuzzo

et al. 2013

PLoS ONE

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The effect of inhomogeneous static magnetic field (SMF)-exposure on the production of different cytokines from human peripheral blood mononuclear cells (PMBC), i.e., lymphocytes and macrophages, was tested in vitro. Some cultures were activated with lipopolysaccharide (LPS) at time point −3 h and were either left alone (positive control) or exposed to SMF continuously from 0 until 6, 18, or 24 h. The secretion of interleukin IL-6, IL-8, tumor necrosis factor TNF-α, and IL-10 was tested by ELISA. SMF-exposure caused visible morphological changes on macrophages as well as on lymphocytes, and also seemed to be toxic to lymphocytes ([36.58; 41.52]%, 0.308≤p≤0.444), but not to macrophages (<1.43%, p≥0.987). Analysis of concentrations showed a significantly reduced production of pro-inflammatory cytokines IL-6, IL-8, and TNF-α from macrophages compared to negative control ([56.78; 87.52]%, p = 0.031) and IL-6 compared to positive control ([45.15; 56.03]%, p = 0.035). The production of anti-inflammatory cytokine IL-10 from macrophages and from lymphocytes was enhanced compared to negative control, significantly from lymphocytes ([−183.62; −28.75]%, p = 0.042). The secretion of IL-6 from lymphocytes was significantly decreased compared to positive control ([−115.15; −26.84]%, p = 0.039). This massive in vitro evidence supports the hypotheses that SMF-exposure (i) is harmful to lymphocytes in itself, (ii) suppresses the release of pro-inflammatory cytokines IL-6, IL-8, and TNF-α, and (iii) assists the production of anti-inflammatory cytokine IL-10; thus providing a background mechanism of the earlier in vivo demonstrated anti-inflammatory effects of SMF-exposure.

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“…According to the results of these studies, MF activates natural killer cells (De Seze et al 1993), stimulates IL-4 synthesis (Salerno et al 1999), reduces the severity of pain (Colbert et al 2008), and when antitumour drugs were used in combination with a weak MF the tumorigenesis was suppressed (Omote et al 1990). In addition, thermal radiation has been known to play a very important role for a very long time in the treatment of various diseases.…”

Section: Effect Of Magnetic Field On Enzyme Activitymentioning

confidence: 99%

Investigation of a weak magnetic field effect on the in vitro catalytic activity of adenosine deaminase and xanthine oxidase

Batçıoğlu

Doğan

Uyumlu

et al. 2012

gpb

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Abstract. The effect of a weak magnetic field (MF) on adenosine deaminase (ADA) and xanthine oxidase (XOD) activities have been investigated. A 50 Hz uniform MF was generated, and the magnitude of the field was kept constant at 5.8 mT. The changes in ADA activity over time were significantly different in and out of the MF; MF caused a steeper decline in ADA activity compared to the situation when no MF is present. In addition, MF caused a significant increase in XOD activity. There were no significant time-related changes for either enzyme in the absence of the MF. We suggest that a weak MF affects enzymatic systems.

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Static magnetic fields generated by a 0.5T MRI unit affects in vitro expression of activation markers and interleukin release in human peripheral blood mononuclear cells (PBMC)

Abstract: The SMF generated by a 0.5 T superconducting MRI unit modified in vitro activation marker expression and interleukin release from human PBMC.

Cited by 21 publications

References 8 publications

A static magnetic field attenuates lipopolysaccharide-induced neuro-inflammatory response via IL-6-mediated pathway

A static magnetic field attenuates lipopolysaccharide-induced neuro-inflammatory response via IL-6-mediated pathway

In Vitro Analysis of the Anti-Inflammatory Effect of Inhomogeneous Static Magnetic Field-Exposure on Human Macrophages and Lymphocytes

Investigation of a weak magnetic field effect on the in vitro catalytic activity of adenosine deaminase and xanthine oxidase

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Static magnetic fields generated by a 0.5T MRI unit affects in vitro expression of activation markers and interleukin release in human peripheral blood mononuclear cells (PBMC)

Abstract: The SMF generated by a 0.5 T superconducting MRI unit modified in vitro activation marker expression and interleukin release from human PBMC.

Cited by 21 publications

References 8 publications

A static magnetic field attenuates lipopolysaccharide-induced neuro-inflammatory response via IL-6-mediated pathway

A static magnetic field attenuates lipopolysaccharide-induced neuro-inflammatory response via IL-6-mediated pathway

In Vitro Analysis of the Anti-Inflammatory Effect of Inhomogeneous Static Magnetic Field-Exposure on Human Macrophages and Lymphocytes

Investigation of a weak magnetic field effect on the in vitro catalytic activity of adenosine deaminase and xanthine oxidase

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Investigation of a weak magnetic field effect on the in vitro catalytic activity of adenosine deaminase and xanthine oxidase