Statically controlled mycogenic-synthesis of novel biologically active silver-nanoparticles using Hafr Al-Batin desert truffles and its antimicrobial efficacy against pathogens

Alshammari, Shifaa O.; Aty, Abeer A. Abd El

doi:10.1016/j.sjbs.2022.103334

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…In another work, temperature, AgNO 3 , and the extract/AgNO 3 ratio were used as independent factors for nanoparticle synthesis using Punica granatum leaves [9]. Two factors, namely AgNO 3 concentration and pH, were used under RSM for nanoparticle synthesis from the extract of desert truffle Ascocarps [10]. Box-Behnken [11] and Plackett-Burman [12] designs under response surface methodology were also used.…”

Section: Introductionmentioning

confidence: 99%

Full Factorial Design Synthesis of Silver Nanoparticles Using Origanum vulgare

Rigopoulos,

Gkaliouri,

Sakavitsi

et al. 2023

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Green synthesis of silver nanoparticles (AgNPs) involves a reduction reaction of a metal salt solution mixed with a plant extract. The reaction yield can be controlled using several independent factors, such as extract and metal concentration, temperature, and incubation time. AgNPs from Origanum vulgare (oregano) were synthesized in the past. However, no investigations were performed on the combined effects of independent factors that affect the synthesis. In this work, silver nitrate, oregano extract, and sodium hydroxide (NaOH) concentrations were chosen as the independent factors, and full factorial design under Response Surface Methodology was employed. UV–Vis absorbance spectroscopy, X-ray Powder Diffraction (XRD), and Fourier Transform Infrared Spectroscopy (FTIR) were used to characterize the nanoparticles. A Voigt function was fitted on the measured UV–Vis spectra. The fitting parameters of the Voigt function, peak wavelength, area, and Full Width at Half Maximum, were used as the responses. A quadratic model was fitted for the peak wavelength and area. The NaOH concentration proved to be the dominant factor in nanoparticle synthesis. UV–Vis absorbance showed a characteristic plasmon resonance of AgNPs at 409 nm. XRD verified the crystallinity of the nanoparticles and FTIR identified the ligands involved.

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Section: Introductionmentioning

confidence: 99%

Full Factorial Design Synthesis of Silver Nanoparticles Using Origanum vulgare

Rigopoulos,

Gkaliouri,

Sakavitsi

et al. 2023

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“…High negative values of AgNPs-RG1 and YG1 appeared clearly to be advantageous for long-term colloidal stability, as mentioned by Eltarahony et al (2018), who indicated that the values less than (−25 mV) or greater than (+25 mV) display higher electrical charge on nano-particles surface, which prevents agglomeration, but Alshammari and Abd El Aty (2022), showed slightly low surface charges of -6.70, -8.73 and 0.16 mv…”

Section: Dynamic Light Scatter Analysis (Dls)mentioning

confidence: 75%

Astragalus sarcocolla Gum-mediated a Novel Green-synthesis of Biologically Active Silver-Nanoparticles with Effective Anticancer and Antimicrobial activities

2023

JJBS

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Cancer diseases and microbial infections are the subject of several ongoing recent researches. In this paper, aqueous extract of red and yellow Astragalus sarcocolla gum was used as safe and green agent, for the synthesis of novel biologically active silver-nanoparticles. Six silver nanoparticles (AgNPs) with different characters were obtained using sarcocolla gum at (5, 10 and 20 mg/ml (w/v)) concentrations. All biosynthesized AgNPs were characterized using UV-visible spectroscopy, Transmission Electron Microscopy (TEM), and Dynamic light scattering (DLS). Cytotoxic effect of sarcocolla gum-AgNPs against five human cancer cells and one normal cells was determined using the MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5diphenyltetrazolium bromide) assay. The semi rounded particles formed from 5mg/ml red gum (AgNPs-RG1) and having 9.23±3.66 nm size range were the most active and possessed high cytotoxic effect against, A549 lung, pc3 prostate, Mcf-7 breast and paca2 pancreatic cancers, with IC 50 values (88.4,75.6, 72.2& 55.0 ug/ml, respectively), but showed low cytotoxic effect (20 % at 100ug/ml) against human normal cells (BJ-1). On the other hand, the spherical to cubic shaped nanoparticles obtained from 5mg/ml yellow gum (AgNPs-YG1) with 10.92 ± 5.48 nm size range, showed good cytotoxic effect against three cancer cells (PC3 prostate, MCF-7 breast and paca2 pancreatic) with IC 50 values (75.0, 82.8 and 81,6ug/ml respectively), and normal cells(BJ-1) with IC 50 value (81.8ug/ml). Antibacterial and antifungal activities of the synthesized red and yellow gum-mediated AgNPs with different sizes and shapes were also investigated. Particles biosynthesized from 5 and 10 mg/ml red gum (AgNPs-RG1, RG2) and 5mg/ml yellow gum (AgNPs-YG1) exhibited broad spectrum antimicrobial effects against all tested pathogenic gram-positive, gram-negative bacterial strains, unicellular yeast and filamentous fungi. Based on these results, it can be concluded that the synthesized silver nanoparticles from low concentrations of sarcocolla gum have significant biomedical applications.

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