Stationary deformations of erythrocytes by high-frequency electric field

Kononenko, V. L.; Shimkus, J. K.

doi:10.1016/s0302-4598(00)00101-x

Cited by 13 publications

(8 citation statements)

References 14 publications

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“…Furthermore, the polarization of the cell membrane under an external electric field exerts local forces (Krassowska and Neu 1994) which cause various deformational regimes of cell elongation (Kononenko and Shimkus 2000) and can transform the spherical cell into prolate ellipsoids if the membrane surface is sufficiently flexible (Neek-Amal et al 2009). However, for simplification, they are generally considered as spherical particles (Kadaksham et al 2005;Urdaneta and Smela 2007).…”

Section: Theoretical Backgroundmentioning

confidence: 99%

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Khoshmanesh

Zhang

Tovar-Lopez

et al. 2009

Microfluid Nanofluid

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This article presents the numerical and experimental analysis of a dielectrophoretic-activated cell sorter (DACS), which is equipped with curved microelectrodes. Curved microelectrodes offer unique advantages, since they create strong dielectrophoretic (DEP) forces over the tips and maintain it over a large portion of their structure, as predicted by simulations. The performance of the system is assessed using yeast (Saccharomyces cerevisiae) cells as model organisms. The separation of the live and dead cells is demonstrated at different medium conductivities of 0.001 and 0.14 S/m, and the sorting performance was assessed using a second array of microelectrodes patterned downstream the microchannel. Further, microscopic cell counting analysis reveals that a single pass through the system yields a separating efficiency of *80% at low medium conductivities and *85% at high medium conductivities.

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Section: Theoretical Backgroundmentioning

confidence: 99%

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Khoshmanesh

Zhang

Tovar-Lopez

et al. 2009

Microfluid Nanofluid

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“…Recent progress in the study of the dielectrophoretic cell-deformation investigations focused on the shape transformation of red blood cells and white blood cells to investigate the activation of their membrane-bound proteins or repairment mechanisms to deformations due to chemical and physical changes that interfere with their cytoskeleton-bilayer [ 34 , 35 , 36 , 37 , 38 , 39 ]. Among these studies, Gass and co-workers investigated the mechanical properties of human red blood cells and their shape transformations due to the local deformations induced by high-frequency electric field [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

Quantifying Deformation and Migration Properties of U87 Glioma Cells Using Dielectrophoretic Forces

Elitaş

Islam²,

Korvink³

et al. 2022

Biosensors

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Glioblastoma multiforme is one of the most aggressive malignant primary brain tumors. To design effective treatment strategies, we need to better understand the behavior of glioma cells while maintaining their genetic and phenotypic stability. Here, we investigated the deformation and migration profile of U87 Glioma cells under the influence of dielectrophoretic forces. We fabricated a gold microelectrode array within a microfluidic channel and applied sinusoidal wave AC potential at 3 Vpp, ranging from 30 kHz to 10 MHz frequencies, to generate DEP forces. We followed the dielectrophoretic movement and deformation changes of 100 glioma cells at each frequency. We observed that the mean dielectrophoretic displacements of glioma cells were significantly different at varying frequencies with the maximum and minimum traveling distances of 13.22 µm and 1.37 µm, respectively. The dielectrophoretic deformation indexes of U87 glioma cells altered between 0.027–0.040. It was 0.036 in the absence of dielectrophoretic forces. This approach presents a rapid, robust, and sensitive characterization method for quantifying membrane deformation of glioma cells to determine the state of the cells or efficacy of administrated drugs.

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“…The reversible deformation is noticed at a low-intensity AC electric field, and the hemolysis of the cells are observed at a high-intensity field [Engelhardt and Sackmann, 1988, Gass et al, 1991, Krueger and Thom, 1997]. Moreover, the deformation also depends upon the conductivity of internal and external fluids [Sukhorukov et al, 1998, Kononenko and Shimkus, 2000. Apart from electrodeformation, electrorotation and its dependency on conductivities of the fluids as well as the frequency of the applied AC electric field is observed.…”

Section: Introductionmentioning

confidence: 99%

“…Attempts have been made to calculate Maxwell stresses at the interface of an erythrocyte [Sebastián et al, 2006]. An improved understanding of the mechanics of RBC has enabled development of better medical diagnostic devices [Engelhardt and Sackmann, 1988, Kononenko and Shimkus, 2000, Thom and Gollek, 2006, Thom, 2009, Du et al, 2014. A similar analysis can also be employed to measure the mechanical properties of the synthetic biconcave-discoid capsule.…”

Section: Introductionmentioning

confidence: 99%

Deformation of a biconcave-discoid capsule in extensional flow and electric field

2018

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Biconcave-discoid (empirical shape of a red blood cell) capsule finds numerous applications in the field of bio-fluid dynamics and rheology, apart from understanding the behavior of red blood cells (RBC) in blood flow. A detailed analysis is, therefore, carried out to understand the effects of the uniaxial extensional flow and electric field on the deformation of a RBC and biconcave-discoid polymeric capsule in the axisymmetric regime. The transient deformation is computed numerically using axisymmetric boundary integral method for Stokes flow considering the Skalak membrane constitutive law as the model for the area incompressible RBC/biconcave-discoid capsule membrane. A remarkable biconcave-discoid to prolate spheroid transition is observed when the elastic energy is overcome by the viscous or Maxwell electric stresses. Moreover, the significance of membrane stresses developed during the deformation and at steady state and different modes of deformation are presented. This study should be useful in designing an artificial system involving biological cells under an electric field. arXiv:1801.01971v2 [cond-mat.soft]

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Stationary deformations of erythrocytes by high-frequency electric field

Cited by 13 publications

References 14 publications

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Dielectrophoretic-activated cell sorter based on curved microelectrodes

Quantifying Deformation and Migration Properties of U87 Glioma Cells Using Dielectrophoretic Forces

Deformation of a biconcave-discoid capsule in extensional flow and electric field

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