Stationary phase with specific surface properties for the separation of estradiol diastereoisomers

Buszewski, Bogusław; Jezierska-Switala, M.; Kowalska, Sylwia

doi:10.1016/s1570-0232(03)00283-6

Cited by 51 publications

(23 citation statements)

References 27 publications

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“…Several tailor-made stationary phases, such as, cholesteryl-based, carbazole-based, molecularly imprinted polymers, temperature responsive phases were used for the separation of steroids and their isomers [43][44][45][46][47][48][49][50][51][52][53]. Figure 4 shows the separation of a mixture of eight steroids having a wide range of polarities on the newly developed alternating copolymer-based Sil-poly(ODA-alt-DGMI) column.…”

Section: Separation Of Steroidsmentioning

confidence: 99%

Selectivity enhancement for the separation of tocopherols and steroids by integration of highly ordered weak interaction sites along the polymer main chain

et al. 2012

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A novel alternating copolymer-based organic phase was synthesized using a new N-substituted maleimide monomer for the development of alternating copolymer-grafted silica for high-performance liquid chromatographic applications. This new monomer (DGMI) was copolymerized with octadecyl acrylate (ODA) from 3-mercaptopropyltrimethoxysilane-grafted silica to produce Sil-poly(ODA-alt-DGMI). The organic phase was characterized by the elemental analysis and the diffuse reflectance infrared Fourier transform spectroscopy. Tocopherol isomers and steroids were used as analytes for the evaluation of the chromatographic selectivity profiles of this novel stationary phase. The selectivity of this column was then compared with a polymeric ODS column and previously developed another alternating copolymer-grafted silica (without the glutamide-derived moiety) column, Sil-poly(ODA-alt-N-octadecylmaleimide). The complete baseline separation of tocopherol isomers in an isocratic mode has been achieved within 25 min with the Sil-poly(ODA-alt-DGMI). The separation of eight kinds of estrogenic steroids and corticoids has also been achieved in an isocratic mode with this column. Significant differences in separation selectivity between Sil-poly(ODA-alt-DGMI) and polymeric ODS columns were observed towards the steroids, and compared with the reference columns, a better separation profile for these analytes was obtained with the Sil-poly(ODA-alt-DGMI). The results of this investigation indicated that the enhancement of selectivity of Sil-poly(ODA-alt-DGMI) towards the test analytes arose from the multiple interaction mechanism such as hydrophobic effect, carbonyl-π and hydrogen-bonding interactions, and such integrated interactions originated from the addition of two amide groups in the N-substituted maleimide monomer.

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Section: Separation Of Steroidsmentioning

confidence: 99%

Selectivity enhancement for the separation of tocopherols and steroids by integration of highly ordered weak interaction sites along the polymer main chain

et al. 2012

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“…[154][155][156] Excellent separation of the epimers of estradiol was achieved by modifying the surface of silica gel with the covalent binding of cholesterol. 157 Molecularly imprinted artificial receptors as HPLC stationary phases were prepared among others for the screening of corticosteroids 158 and estrogens. 159 Porous graphitic stationary phases were also used for the separation of corticosteroids especially if a voltage is applied to the column, while making use of its electric conductivity.…”

Section: ·4 High-performance Liquid Chromatography (Hplc) Andmentioning

confidence: 99%

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

Gǒrög¹

2004

ANAL. SCI.

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“…Our laboratory participated in a CRL-RIVM proficiency study (estradiol in samples of bovine serum) requesting the determination and quantification of 17␣/17␤-E2 in bovine serum [7]. While the separation of estradiol diastereoisomers has been reported [8], and there are several methods for the analysis of 17␤-E2 [9,10], to the best of our knowledge there have been no reports on their chromatographic separation and quantification in bovine serum.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of 17α-estradiol and 17β-estradiol in bovine serum by liquid chromatography–tandem mass spectrometry

Ferretti

Ferranti

Crovella

et al. 2008

Journal of Chromatography B

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Stationary phase with specific surface properties for the separation of estradiol diastereoisomers

Cited by 51 publications

References 27 publications

Selectivity enhancement for the separation of tocopherols and steroids by integration of highly ordered weak interaction sites along the polymer main chain

Selectivity enhancement for the separation of tocopherols and steroids by integration of highly ordered weak interaction sites along the polymer main chain

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

Simultaneous analysis of 17α-estradiol and 17β-estradiol in bovine serum by liquid chromatography–tandem mass spectrometry

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