Stationary Phases 45(1). Chromatographic Separation of High Energetic Materials with C60-Fullerene Stationary Phase

Abstract

A method for the chromatographic separation of nitroaromatic high energetic materials on C60‐fullerene stationary phase is discussed. Special selectivity on this phase using methanol/water(80/20) as mobile phase leads to have a wider separating space so that its retention power is much greater than that of a commercial RP‐18 phase. This advantage can be used to identify the isomers of some nitroaromatic high energetic materials.

Nano-particle modified stationary phases for high-performance liquid chromatography

“…For example, Chang et al, have reported the immobilisation of C 60 -fullerenes on silica substrates, and applied the modied phase as a reversed-phase substrate for use in the HPLC separation of high energetic materials (explosives). 47 The retention behaviour for a total of 23 explosives was studied, and a well resolved separation of 6 explosives was achieved in $35 min, using isocratic elution with methanol-water (80/20) mixture. This was directly compared to the separation of the same analytes, under the same conditions on an ODS stationary phase, where no separation was achieved due to co-elution of 4 of the 6 analytes.…”

“…These results clearly highlight the much higher specicity for substituted aromatic compounds afforded by the immobilised fullerenes, and is supported by similar studies reporting high selectivity for PAHs using such phases. 48 More recently, Bonn and co-workers have explored the selectivity of C 60 -fullerene modied silica gels, using a similar immobilisation approach to that of Chang et al, 47,48 but here fullerenes were immobilised onto 3-aminopropylsilica (APS) using either C 60 -fullerenoacetic acid or C 60 -epoxyfullerene as the starting materials. [49][50][51] Characterising selectivity of the modied phases towards various biomolecules, including amino acids, carbohydrates, proteins, peptides and phospholipids was the main aim of this work, and particular selectivity for phosphorylated peptides was noted by the researchers.…”