Stationary phases for packed-column supercritical fluid chromatography

Development of supercritical fluid chromatography coupled with mass spectrometry method for characterization of a nonionic surfactant and comparison with liquid chromatography coupled with mass spectrometry method

“…Because of strong dispersion force with stationary phase (C18 column), the series d (PEO‐glu‐tetrastearate) was not eluted. Relative to RPLC, the stationary phase of SFC was pure silanol column, the retention mechanism of which was similar to normal phase LC system . The resolution (R) of SFC is better than resolution of LC in same series.…”

Fractionation of Mangifera indica Linn polyphenols by reverse phase supercritical fluid chromatography (RP-SFC) at pilot plant scale

“…All stationary phases used in HPLC, including less polar or modified columns, are useful for SFC. However, there are no defined applications or indications about the packing material appropriate for a sample type [20,27]. Orthogonal studies in order to define the most suitable applicability of different types of packing materials, also used in reverse-phase separations, such as phenyl, cyanopropyl, diol, polar embedded or endcapped columns, in SFC have been recently reported [27][28][29][30][31][32].…”

“…However, there are no defined applications or indications about the packing material appropriate for a sample type [20,27]. Orthogonal studies in order to define the most suitable applicability of different types of packing materials, also used in reverse-phase separations, such as phenyl, cyanopropyl, diol, polar embedded or endcapped columns, in SFC have been recently reported [27][28][29][30][31][32]. Anyway, few developed procedures for natural substances separation by SFC with reverse phase C18 (RP-C18) column have been reported [26,32,33].…”

A novel method for characterization and comparison of reversed-phase column selectivity

“…More information of these tests has been thoroughly reviewed in the literature [4]. To systematically characterize and compare reversed-phase stationary phases, a universal method based on linear solvation energy relationships (LSERs) was introduced and developed by Kamlet [10,11], Abraham [12,13] and Poole [14,15]. The LSERs equation describes the relationship between the retention factors of probe solutes and their descriptors in a given separation system [16]: ln k = c + eE + sS + aA + bB + vV (1) http://dx.doi.org/10.1016/j.chroma.2014.08.005 0021-9673/© 2014 Elsevier B.V. All rights reserved.…”