[1] In a companion paper, we derived the high-frequency, compressible, dissipative polarization relations for gravity waves (GWs) propagating in the thermosphere. In this paper, we apply the results to nighttime thermospheric observations of a GW over Alaska on 9-10 January 2010. Using a vertically-pointed Fabry-Perot interferometer (FPI) at Poker Flat that measured vertical wind perturbations (w′) and two FPIs that measured the line-of-sight (LOS) velocities in four common volumes, we inferred a GW ground-based period $32.7 AE 0.3 min, horizontal wavelength l H = 1094 AE 408 km, horizontal ground-based phase speed c H $ 560 AE 210 m/s, and propagation azimuth q $ 33.5 AE 15.8 east-of-north. We compared the phase shifts and amplitude ratios of this GW with that predicted by the GW dissipative polarization relations derived in the companion paper, enabled by the ability of the FPIs to measure fundamental GW parameters (wind and temperature perturbations). We find that GWs with l H $ 700-1100 km, l z $ À500 to À350 km, q $ 15 to 50, and c H $ 350-560 m/s agree with the observations if the primary contribution to the 630-nm emission was near the upper portion of that layer. The source of GW was likely thermospheric given the large intrinsic phase speed of the wave. Possible sources are discussed, the most likely of which are related to the onset of auroral activity near the time that the wave was initially observed.