Statistical Evaluation of Diluents and Automatic Diluting and Pipetting Machines in Influenza Serology

O’Brien, Thomas C.; Rastogi, Suresh Chandra; Tauraso, Nicola M.

doi:10.1128/aem.21.2.311-315.1971

Cited by 3 publications

(1 citation statement)

References 4 publications

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“…Antibody determination. The antibody determination was performed (Tables 1 and 2) with the hemagglutination inhibition test, using the microtiter method (7,12) in "V" plates in the automatic pipetting machine (Autotiter III, manufactured by Canalco) with 0.5% chicken erythrocytes.…”

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New adjuvants on a polymethylmethacrylate base

Kreuter¹,

Speiser²

1976

Infect Immun

121

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A new type of adjuvant using influenza virus as an antigen is presented. The new adjuvant was produced by polymerizing monomeric methylmethacrylate in the presence of the antigen. As a comparison, influenza virus was added to previously polymerized polymethylmethacrylate particles. In animal experiments, the antibody response in mice and guinea pigs was measured. After polymerization in the presence of the antigen, the adjuvant effect was dependent on the methylmethacrylate concentration used, reaching an optimal concentration at 0.5%. This adjuvant preparation was considerably more effective than simple addition of virus to comparable polymethacrylate preparations or to aluminum hydroxide. The latter two adjuvants were approximately equivalent in effectiveness.

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Section: Downloaded Frommentioning

confidence: 99%

New adjuvants on a polymethylmethacrylate base

Kreuter¹,

Speiser²

1976

Infect Immun

121

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Antibodies to type A influenza viruses in sera from nonhuman primates

O'brien

Tauraso

1973

Archiv f Virusforschung

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Vaccinia virus: Kinetics of the hemagglutination-inhibition test and preparation of hemagglutinin

O’Brien

Taurkaso

1972

Archiv f Virusforschung

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SummaryThe optimum temperature and time of incubation of the vaccinia hemagglutination-inhibition test were 37~ and 16 hours, respectively. A convenient method for preparing vaccinia hemagglutinating antigen consisted of infecting one-day-old primary chick embryo fibroblasts with a high multiplicity of vaccinia virus, employing a maintenance medium devoid of serum, preparing a 20-fold concentration of virus-infected cells, and finally extracting the concentrated antigen homogenate with genetron.

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Statistical Evaluation of Diluents and Automatic Diluting and Pipetting Machines in Influenza Serology

Cited by 3 publications

References 4 publications

New adjuvants on a polymethylmethacrylate base

New adjuvants on a polymethylmethacrylate base

Antibodies to type A influenza viruses in sera from nonhuman primates

Vaccinia virus: Kinetics of the hemagglutination-inhibition test and preparation of hemagglutinin

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