Statistical informatics: software tools for statistical inference about expression arrays

Abstract

Poster abstracts 65 cations to oncology research we have developed the AmpliOnc TM I array, which contains 3 spots for each of over 50 target loci (mostly oncogenes) that have been shown to be potentially amplified in tumour tissues. In hybridizations of normal female (green) against normal male (red) total human DNA, the spots containing the androgen receptor (X chromosome) revealed a green/red ratio significantly higher than the ratios of all other loci (>99% confidence level). This suggests sufficient sensitivity for single copy-change detection. In tests with defined tumour cell lines, the expected ratios were obtained for multiple amplifications with more than 95% confidence on each chip. Multiple tumour cell lines and primary tumours have been analysed with the AmpliOnc TM chip. In all cases known oncogene amplifications were confirmed, and in some instances additional and so far unknown amplifications were discovered.