Statistical media optimization studies for growth and polydroxybutyrate (PHB) production by Pseudomonas spp.

Abstract

Using glucose as carbon source and mustard cake and yeast extract as nitrogen sources bacterial isolate Pseudomonas B2 exhibited a maximum PHB recovery of 0.620 (in terms of O.D.) and PHB weight of 0.27g/L in 96 h. To determine the possibility of growth potential of Pseudomonas spp., it was grown in different carbon sources like fructose, glucose, maltose, mannitol etc. and it was found that glucose yielded good growth and PHB production. In order to incorporate cost effective nitrogen and carbon source, mustard cake and cotton cake as nitrogen source and molasses as carbon were used in medium. Statistical media optimization design was used to optimize the culture conditions for maximizing the PHB production. A maximum of 0.37 g/L of PHB and 0.746 (O.D.) PHB recoveries were obtained using optimized concentrations. Batch kinetics can be used for model development, which will make possible simulation of nutrient limited cultivation(s) for over accumulation of PHB. FTIR studies confirmed the presence of PHB.

Neoteric Solvent Systems as Sustainable Media for Dissolution and Film Preparation of Poly-[(R)-3-hydroxybutyrate]

“…All of the regenerated samples showed all characteristic peaks corresponding to those observed for standard PHB indicating preservation of chemical structure during dissolution in the ILs. 18,29 However, it may be noted that the characteristic band of the CO streching vibration observed at 1724 cm −1 is a bit weaker in the case of the regenerated polymers in comparison to the standard sample, which is perhaps due to the interaction of the CO group with the cation of the ionic liquids having an amine group. Further molecular docking studies which are discussed later showed that the oxygen of (CO) tends to form H-bonding with the anions of ILs.…”