Statistical optimisation of xylanase production by estuarine Streptomyces sp. and its application in clarification of fruit juice

Abstract

Recently, xylanase has become an essential option for environmental friendly industrial biotechnological applications and the rising demand for its large scale production urge to take the advantage of statistical approach of optimization to investigate the interactive effects of prominent process factors involved to enhance xylanase production. In the present study, xylanase production from Streptomyces sp. strain ER1 isolated from Cochin estuarine soil; was optimised using statistical designs- Plackett-Burman and Central composite design. Plackett-Burman design was used to identify important fermentation condition factors affecting the xylanase production using beechwood xylan as the substrate. The optimum levels of these significant factors were determined employing the Central Composite Design. Out of the thirteen factors screened, concentration of beechwood xylan and olive oil, agitation speed, and inoculum age were recognized as the most significant factors. By analyzing the response surface plots and using numerical optimization method, the optimal levels for concentration of xylan and olive oil, agitation speed and inoculum age were determined as 0.37%, 33.10 mg/L, 42.87 RPM and 21.05 h, respectively. The optimised medium resulted in a 1.56-fold increased level of the xylanase (10,220 U/mL) production compared to the initial level (3986.444 U/mL) after 120 h of fermentation. The purified enzyme could successfully clarify orange, mousambi and pineapple juice to 20.87%, 23.64% and 27.89% respectively. Thus the present study has proved that Streptomyces sp. strain ER1 (KY449279) is a potential and useful organism for xylanase production and its purified enzyme could clarify the selected fruit juices.

A detailed overview of xylanases: an emerging biomolecule for current and future prospective

“…This increases the clarity, aroma, and color of the juice (Danalache et al 2018). Xylanase from Streptomyces sp was used for the clarification of orange, mousambi, and pineapple with 20.9%, 23.6% and 27.9% clarity, respectively (Rosmine et al 2017). Immobilized xylanase obtained from Bacillus pumilus VLK-1 was used for orange (29%) and grape juice (26%) enrichment .…”

Statistical optimization of xylanase production, using different agricultural wastes by Aspergillus oryzae MN894021, as a biological control of faba bean root diseases

“…Some of the most commonly agro-residues used as a cheap and renewable carbon source for xylanase production and for developing biotechnological processes of industrial interest; as wheat bran (El Shamy et al 2016), wheat husk (Kumar et al 2018), different and numerous of agricultural wastes, and different vegetable leaf industries and groundnut shell (Rosmine et al 2017;Sindhu et al 2017). A recent study also showed that wastewater from the pulp industry was reused as medium for xylanase production (de Queiroz-Fernandes et al 2017).…”

Diversity and Biotechnological Potential of Xylan-Degrading Microorganisms from Orange Juice Processing Waste

“…Reports on the induction of xylanases from allochthonous bacteria growing in orange processing (by)products are limited in number, while no work on indigenous xylanolytic microbiota isolated from orange juice processing waste exists in the international literature. Streptomyces [40][41][42] and Bacillus/Geobacillus [43,44] spp. isolated from soil are common bacterial strains that have found to exhibit endo-β-1,4-xylanase activities when tested on orange processing (by)products.…”