Statistical optimization of chitosanase production by Aspergillus sp. QD-2 in submerged fermentation

Zhang, Hui; Sang, Qing; Zhang, Wenhui

doi:10.1007/s13213-011-0246-1

Cited by 35 publications

(18 citation statements)

References 33 publications

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“…L-DOPA is used as a precursor for the production of dopamine which is a potent drug for the treatment of Parkinson’s disease and to control the myocardium neurogenic injury (Ikram-ul-Haq et al 2002 ; Zaidi et al 2014 ). Chitosanase (EC 3.2.1.132) catalyze hydrolysis of chitosan to biologically active chitosan oligosaccharides (COS), which is used as antimicrobial, antioxidant, lowering of blood cholesterol and high blood pressure, controlling arthritis, protective effects against infections and improving antitumor properties (Kim and Rajapakse 2005 ; Ming et al 2006 ; Zhang et al 2012 ; Thadathil and Velappan 2014 ). Applications of microbial enzymes for different health problems are illustrated in Table 3 (Devlin 1986 ; Kaur and Sekhon 2012 ; Sabu 2003 ; Vellard 2003 ; Mane and Tale 2015 ).…”

Section: The Use Of Enzymes In Industrial Processesmentioning

confidence: 99%

Microbial enzymes: industrial progress in 21st century

et al. 2016

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Biocatalytic potential of microorganisms have been employed for centuries to produce bread, wine, vinegar and other common products without understanding the biochemical basis of their ingredients. Microbial enzymes have gained interest for their widespread uses in industries and medicine owing to their stability, catalytic activity, and ease of production and optimization than plant and animal enzymes. The use of enzymes in various industries (e.g., food, agriculture, chemicals, and pharmaceuticals) is increasing rapidly due to reduced processing time, low energy input, cost effectiveness, nontoxic and eco-friendly characteristics. Microbial enzymes are capable of degrading toxic chemical compounds of industrial and domestic wastes (phenolic compounds, nitriles, amines etc.) either via degradation or conversion. Here in this review, we highlight and discuss current technical and scientific involvement of microorganisms in enzyme production and their present status in worldwide enzyme market.Graphical abstract

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Section: The Use Of Enzymes In Industrial Processesmentioning

confidence: 99%

Microbial enzymes: industrial progress in 21st century

et al. 2016

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“…This saves cost and time and makes RSM more effective than classical methods [ 26 ]. RSM has been applied to various cases of enzyme production [ 27 – 29 ]. In recent years, proteolytic enzymes have attracted much more attention for their hydrolytic activity toward recalcitrant animal proteins such as keratin [ 30 ] and amyloid prion proteins [ 31 ] and collagen [ 32 ] and blood clot [ 33 ] and management of industrial and medical wastes.…”

Section: Introductionmentioning

confidence: 99%

Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme byBacillussp. IND12 Using Response Surface Methodology in Solid-State Fermentation

Vijayaraghavan

Rajendran

Vincent

et al. 2017

BioMed Research International

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Fibrinolytic enzymes have wide applications in clinical and waste treatment. Bacterial isolates were screened for fibrinolytic enzyme producing ability by skimmed milk agar plate using bromocresol green dye, fibrin plate method, zymography analysis, and goat blood clot lysis. After these sequential screenings, Bacillus sp. IND12 was selected for fibrinolytic enzyme production. Bacillus sp. IND12 effectively used cow dung for its growth and enzyme production (687 ± 6.5 U/g substrate). Further, the optimum bioprocess parameters were found out for maximum fibrinolytic enzyme production using cow dung as a low cost substrate under solid-state fermentation. Two-level full-factorial experiments revealed that moisture, pH, sucrose, peptone, and MgSO4 were the vital parameters with statistical significance (p < 0.001). Three factors (moisture, sucrose, and MgSO4) were further studied through experiments of central composite rotational design and response surface methodology. Enzyme production of optimized medium showed 4143 ± 12.31 U/g material, which was more than fourfold the initial enzyme production (978 ± 36.4 U/g). The analysis of variance showed that the developed response surface model was highly significant (p < 0.001). The fibrinolytic enzyme digested goat blood clot (100%), chicken skin (83 ± 3.6%), egg white (100%), and bovine serum albumin (29 ± 4.9%).

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“…Various cultivation parameters including carbon source, nitrogen source, inorganic salts, temperature, pH, incubation time, and agitation rate can directly affect chitosanase production . It has been observed that the use of one additional carbon source in combination with CC is beneficial to increase the enzyme activity because the added carbon source help to promote growth of bacteria in the initial period.…”

Section: Discussionmentioning

confidence: 99%

“…Instead, statistical methods are beneficial to evaluate several variables with minimum number of experiments and provide adequate data for satisfactory results. RSM is an effective technique for statistical optimization of multiple variables performing least number of experiments . This technique has been effectively used to optimize medium composition, enzymatic hydrolysis conditions, fermentation processes, and food manufacturing processes .…”

Section: Discussionmentioning

confidence: 99%

Optimization of chitosanase production by Bacillus mojavensis EGE‐B‐5.2i

et al. 2018

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Maximum production of industrially important enzymes such as chitosanases through media optimization still holds foremost interest. The present study was conducted to improve chitosanase activity of an indigenous strain identified as Bacillus mojavensis. Initially, carbon and nitrogen sources were optimized by one-variable-at-a-time approach. Further, fermentation medium was optimized using Plackett-Burman (PB) and central composite designs (CCD). PB verified soluble starch (SS), colloidal chitosan (CC) peptone, and NaCl as most significant variables affecting chitosanase production. CCD results predicted the optimum concentrations of SS, CC, peptone, and NaCl as 7.8, 7.0, 6.5, and 2.7 g L , respectively to achieve maximum chitosanase activity (21.1 U ml ). Discovery of the novel optimal medium has improved chitosanase production by B. mojavensis up-to 9.5 folds. Lastly, 18.6 U ml chitosanase activity was achieved in stirred tank bioreactor using optimal medium, which is quite satisfactory to proclaim this strain as a potential candidate to provide commercial chitosanase.

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Statistical optimization of chitosanase production by Aspergillus sp. QD-2 in submerged fermentation

Cited by 35 publications

References 33 publications

Microbial enzymes: industrial progress in 21st century

Microbial enzymes: industrial progress in 21st century

Novel Sequential Screening and Enhanced Production of Fibrinolytic Enzyme byBacillussp. IND12 Using Response Surface Methodology in Solid-State Fermentation

Optimization of chitosanase production by Bacillus mojavensis EGE‐B‐5.2i

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