Statistical optimization of crude oil bio-degradation by a local marine bacterium isolate Pseudomonas sp. sp48

Abstract

Pseudomonas sp. sp48, a marine bacterium isolated from Bahary area (Alexandria, Egypt), showed a high potency for oil degradation up to 1.5%. Additionally, it showed an ability to consume aromatic hydrocarbons (phenol & naphthalene) and aliphatic (pentadecane) reaching to 79; 73; 62%, respectively. In the current study, Plackett-Burman factorial design was applied to evaluate culture conditions affecting the degradation potency. Analysis of Plackett-Burman design results revealed that, the most significant variables affecting oil removal were magnesium sulfate, inoculum size, glucose and Triton X-100. To optimize the levels of these significant variables Response Surface Methodology (RSM) was followed. In this respect, the three-level Box–Behnken design was employed and a polynomial model was created to correlate the relationship between the three variables and oil removal. The optimal combinations of the major constituents of media that was evaluated from the non-linear optimization algorithm of EXCEL-Solver was as follows: (w/v%) 1 crude oil, 0.5 peptone, 0.5 yeast-extract, 1 ammonium chloride, 0.7418 D-glucose, 0.5 MgSO4·7H2O, 0.1 Triton X-100 and inoculums size 4.18 ml% in natural sea water at pH 7; 30 °C incubation temperature, 200 rpm for 6 days. The predicted optimum oil removal was 89%, which is 2.4 times more than the basal medium.

Microbial Biodegradation of Paraffin Wax in Malaysian Crude Oil Mediated by Degradative Enzymes

“…Nutrients availability could also promote cell growth and degradation of crude oil by improving the production of the enzyme. The increase of bacterial growth and oil degradation with the addition of yeast extract (Okeke and Frankenberger, 2003;Mukred et al, 2008;Palanisamy et al, 2014;Farag et al, 2018), peptone (Mukred et al, 2008), and beef extract (Dixit and Pant, 2000) were previously reported. The addition of only yeast extract was shown to accelerate bacterial growth and allowed sufficient crude oil degradation as compared to other nutrients.…”

Isolation and Characterization of Bacteria from Refinery Effluent for Degradation of Petroleum Crude Oil in Seawater

“…Spillage of oil into marine and fresh water environment causes an increase in carbon content and a drastic decrease in nitrogen content 23 . It has been reported that the addition of a simple carbon source such as glucose increased the biodegradation rate of total petroleum hydrocarbons (TPH) in contaminated samples 24 . Hence in the present study the growth of identified isolates and the biodegradation of PCO were studied under the presence of trace amounts of glucose and yeast extract.…”

Isolation and identification of a psychrotolerant dimethyl phthalate-degrading bacterium from selected frozen soil of high-latitude areas in China and optimization of its fermentation conditions using response surface methodology

“…Based on the contour plots and response surface curves ( Figure 5(a-f)), the fermentation biomass at different levels of the four factors can be predicted [49]. Each contour ellipse plot represents an infinitive number of combinations of two factors, with the other two factors maintained at their central levels [50]. The maximum predicted value is indicated by the response surface confined in the smallest ellipse in the contour diagram.…”