Statistical optimization of culture conditions for biomass production of probiotic gut-borneSaccharomyces cerevisiaestrain able to reduce fumonisin B₁

Abstract

Aim: To evaluate the ability of probiotic Saccharomyces cerevisiae RC016 strain to reduce fumonisin B 1 (FB 1 ) in vitro and to optimize the culture conditions for the growth of the yeast employing surface response methodology. Methods and Results: Using Plackett-Burman screening designs (PBSD) and central composite designs (CCD), an optimized culture medium containing (g l À1 ) fermentable sugars provided by sugar cane molasses (CMs), yeast extract (YE) and (NH 4 ) 2 HPO 4 (DAP) was formulated. The S. cerevisiae RC016 strain showed the greatest binding at all assayed FB 1 concentration. The CMs, YE, DAP concentrations and incubation time influenced significantly the biomass of S. cerevisiae RC016. Conclusion: A combination of CMs 17%; YE 4Á61 g l À1 and incubation time 60 h was optimum for maximum biomass of S. cerevisiae RC016. Significance and Impact of the Study: The importance of this work lies in the search for live strains with both probiotic and fumonisin B 1 decontamination properties that could be sustainably produced in a medium just containing cheap carbon, nitrogen and phosphorus sources and would be included in a novel product to animal feed.

In Vitro Detoxification of Aflatoxin B1, Deoxynivalenol, Fumonisins, T-2 Toxin and Zearalenone by Probiotic Bacteria from Genus Lactobacillus and Saccharomyces cerevisiae Yeast

“…In our research, high and diverse activity of the analysed S. cerevisiae strains reducing FUM concentration was also shown, and the results agree with those of Pizzolitto et al (2012b) [56]. Furthermore, Armando et al (2013), who also observed high FB 1 binding properties of S. cerevisiae yeast (strain RC016), observed that ability of binding the mycotoxin increases along with concentration [59]. Our results also showed that the strains isolated from the feed were characterised by a slightly lower ability to remove FUM (67.43% after 24 h of incubation) than achieved by baker's yeast (70.71-74.47% reduction of FUM concentration after 24 h of incubation) or strains isolated from a distillery environment (72.88% and 73.04% reduction of mycotoxin concentration after 24 h of incubation).…”

Mycotoxins – prevention and decontamination by yeasts

“…Fumonisin B 1 was successfully removed by a S. cerevisiae strain through physisorption as reported by Pizzolitto et al . Fumonisin‐detoxifying organisms are exploitable in the agriculture and an optimization study for cost‐efficient biomass production by a probiotic S. cerevisiae strain was recently published .…”

Food‐Grade Expression and Characterization of a Dextranase from Chaetomium gracile Suitable for Sugarcane Juice Clarification

“…Nitrogen sources are also essential components for the synthesis of microbial cell proteins and nucleic acids and can have an important influence on cell growth and the accumulation of metabolites. [17] The results showed that the strains could utilize both organic and inorganic nitrogen sources for growth and DEX production ( Figure 2B). Among the nitrogen sources tested, casein was found to induce maximum DEX production (38.91 U/mL, P < 0.05) and cell growth (1.378, P < 0.05).…”