Statistical optimization of L-asparaginase production by using Fusarium solani

El-Hadi, Abeer A.; El-Refai, Heba A.; Shafei, Mona S; Zaki, Rania A.; Mostafa, Hanan

doi:10.4103/1687-4315.205825

Cited by 13 publications

(3 citation statements)

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“…Results in the present study revealed that fungal endophyte Fusarium solani isolated from Curcuma longa rhizomes showed L-asparaginase activity of 619.102 IU ml -1 which is higher than which previously reported enzyme activities. Similarity, El Refai et al (2018) and El-Hadi et al (2017) reported F. solani as a potent source of L-asparaginase enzyme with an enzyme activity of 121 U ml -1 and 254 U ml -1 respectively. Gaddad et al (2016) reported that Fusarium sp.…”

Section: Discussionsupporting

confidence: 76%

Screening of Fungal Endophytes Isolated From Medicinal Plants for Glutaminase Free L-Asparaginase Activity

Bhosale¹,

As-Suhbani²

2019

JEBAS

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Endophytes considered as a potent source of naturally produced bioactive compounds. This study was designed to isolate fungal endophytes that are capable of producing Glutaminase free L-asparaginase enzyme from the selected medicinal plants of Nanded city, Maharashtra, India. Four host medicinal plants traditionally known to have antitumour properties, i.e., Curcuma longa, Murraya koenigii, catharanthus roseus and Withania somnifera were selected for this study. Among the total seventy eight (78) isolated fungal endophytes, only five isolates were shown Glutaminase free L-asparaginase production activity. Fusarium solani isolated from Curcuma longa showed the highest enzyme production activity level as compared to others by 619.102 IU ml -1 with 8.807 IU mg -1 specific activity, followed by F.oxysporum (514.532 IU ml -1 with 7.401 IU mg -1 specefic activity), Penicillium sp. (307.114 IU ml -1 with 5.033 IU mg -1 specific activity ), Aspergillus sp. (216.847 IU ml -1 with 4.920 specific activity ) and Alternaria sp. (149.057 IU ml -1 with 3.035 IU mg -1 specific activity). This study demonstrate the importance of using fungal endophytes from antitumour plant hosts as a good and potent alternative source for L-asparaginase production with novel characteristics at high therapeutic index.

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Section: Discussionsupporting

confidence: 76%

Screening of Fungal Endophytes Isolated From Medicinal Plants for Glutaminase Free L-Asparaginase Activity

Bhosale¹,

As-Suhbani²

2019

JEBAS

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“…The graphical illustrations of the model equation which explained the relationship between different levels of pair-wise combination of the studied variables and pigment weight were generated by plotting the three-dimensional response surface plot (3D) and two-dimensional contour plots (2D) ( Figure 4 ) [ 31 ]. The empirical functional relationship is expressed as the pigment weight (response) on Z-axis against coded levels of two independent factors on horizontal axes, while the other explanatory factors are kept at their center point (zero levels).…”

Section: Resultsmentioning

confidence: 99%

Carotegenic Virgibacillus halodenitrificans from Wadi El-Natrun Salt Lakes: Isolation, Optimization, Characterization and Biological Activities of Carotenoids

Fayez

Youssif

Sabry

et al. 2022

Biology

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Carotenoids, as phytonutrient pigments, are signified by their unique beneficial features that serve human health and the surrounding ecosystem. Haloalkaliphiles from soda lakes produce different natural bioactive molecules; however, their ability to produce carotenoids has been limited. Therefore, this study focused on the screening and isolation of carotenoid-producing haloalkaliphilic microbes. Out of 10 isolates, a powerful carotigenic bacterium was isolated, characterized phenotypically and identified on the molecular level as Virgibacillus halodenitrificans. By employing statistical approaches like Plackett–Burman design and central composite design, the influence of significant nutritional variables on carotenoids production was screened and optimized. Predictive modeling manifested that carotenoid yield was 36.42 mg/mL, a 2.12-fold enhancement compared to the basal conditions through inoculating 1.8% of bacterial biomass on optimized medium containing yeast extract (2 g/mL), peptone (10 g/mL) and NaCl (233.6 g/mL). The carotenoids content was confirmed by UV-Vis spectrum which detected a characteristic unique peak with left and right shoulders at 461 nm, 490 and 522 nm. However, FTIR and Raman spectroscopy showed the presence of several functional groups. Meanwhile, LC–MS confirmed that the examined carotenoids were composed of β-carotene, lutein and β-Apo-8′-carotenal mixture. As a bioactive agent, the carotenoids of V. halodenitrificans DASH showed characteristic antagonistic potency against a wide spectrum of Gram-positive and Gram-negative pathogens. Interestingly, a potent antifungal capacity was observed against Candida albicans, reflecting promising mycocidal efficacy against COVID-19 white fungal post-infections. Furthermore, carotenoids (20 μg/mL) inhibited the biofilm formation of P. aeruginosa and S. aureus by 54.01 ± 3.97% and 80.082 ± 0.895%, respectively. Our results proposed that haloalkaliphiles of Wadi El-Natrun lakes are promising sources of carotenoids that exhibited efficiency as safe, biocompatible and natural bioactive agents for environmental, medical and industrial applications.

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“…The bottles were incubated for 96 hours (30 °C and 150 rpm), then to separate the fungal cell mass, centrifuged at 5000 rpm for 15 minutes at 4 °C. The supernatant was used as a raw enzyme source to determine enzyme activity [8,9,10].…”

Section: L-asparaginase Production Via Submerged Fermentationmentioning

confidence: 99%

Semi-quantitative detection for L-asparaginase producing fungi and the impact of carbon and nitrogen sources on enzyme activity

Hamed

Ateş

2021

Preprint

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Objective: To semi-quantitively screen filamentous fungi isolated from different habitats for L-asparaginase production by three indicators; phenol red, cresol red and bromothymol blue and to examine the impact of different carbon and nitrogen sources on the enzyme production using different fungal isolates. Materials and methods: Fifty-five fungal isolates were tested for L-asparaginase production by plate assay using Modified Czapek-Dox (MCD) medium. The enzyme activity was estimated using the Nessler method which measures the concentration of ammonia formed owing to the enzyme action on the substrate. The impact of nitrogen and carbon sources on the enzyme production was done by using the best three L-asparaginase producers from the semi-quantitative screening. Results and conclusions: A total of 53/55 (96.36%) fungal isolates were L-asparaginase producing strains, of them, Cladosporium tenuissimum, Penicillium camembertii and Aspergillus carneus showed high enzyme production. Production of L-asparaginase was higher with the glucose and urea as carbon and nitrogen sources, respectively. The highest enzyme level (5,558 U/ml) was produced by C. tenuissimum in a glucose-containing medium. This study shows that P. camemberti, A. carneus, and C. tenuissimum are good L-asparaginase producers and thus could be used for L-asparaginase production. Keywords: L-asparaginase, anticancer, indicator, fungi, carbon source, nitrogen source.

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Statistical optimization of L-asparaginase production by using Fusarium solani

Cited by 13 publications

References 0 publications

Screening of Fungal Endophytes Isolated From Medicinal Plants for Glutaminase Free L-Asparaginase Activity

Screening of Fungal Endophytes Isolated From Medicinal Plants for Glutaminase Free L-Asparaginase Activity

Carotegenic Virgibacillus halodenitrificans from Wadi El-Natrun Salt Lakes: Isolation, Optimization, Characterization and Biological Activities of Carotenoids

Semi-quantitative detection for L-asparaginase producing fungi and the impact of carbon and nitrogen sources on enzyme activity

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