Statistical optimization of lignocellulosic waste containing culture medium for enhanced production of cellulase by Bacillus tequilensis G9

Abstract

The ever increasing energy demands of modern civilization and rapidly dwindling fossil fuels point towards a renewable substitute like biofuels. However, higher costs associated with biofuel productions is the major bottleneck for its commercialization. The present study demonstrates the use of a statistical approach called response surface methodology (RSM) to investigate the optimum parameters for maximum production of cellulase by Bacillus tequilensis G9. The Plackett-Burman design (PB) of the RSM analysis indicated grass straw (GS) concentration, pH, FeSO 4 , inoculum, MgSO4, incubation period and NH 4 Cl as significant variables that influence the cellulase production. Further, to propose the best medium for the maximum production of cellulase by B. tequilensis G9, the most influential parameters, namely concentrations of GS as substrate, FeSO 4 , pH, inoculum size, etc. were fine-tuned by central composite design (CCD) involving four factors and five levels. The CCD analysis demonstrated 8% substrate concentration, 1.5% of inoculum along with 10 ppm FeSO 4 and a pH of 5.5 in media as optimum conditions for highest enzyme production. The field emission scanning electron microscopic analysis of the treated GS showed structural alterations depicting significant deconstruction caused by B. tequilensis G9. The yield of the partially purified cellulase proteins were found to be 21% revealing molecular mass between 30 and 97 kDa. The enhanced cellulase production by B. tequilensis G9 demonstrated in our study brands its applications in many industrial processes like biorefinery, biofuels, etc.

Using Paper Waste and Local Bacterial Isolates to Produce Biomass and Cellulase Enzyme- A Study Waste Recycling

“…was obtained using a liquid medium containing paper waste as a single carbon source, distribute the medium in 250ml flasks, sterilize the farm and put the bacterial vaccine and incubated for three days, after which the bacterial cultures were filtered using filter papers to separate the portion The steel (precipitate) from the filtrate of the farm in which the enzyme is present. The precipitate was washed with distilled water and dried in the oven at 60 ° C for 24 hours, then the dry biomass was weighed by the sensitive scale to estimate the weight of the dry biomass which represents the final product of the single cell protein [8] Optimization environmental conditions for the growth of selective and degraded bacterial isolates of cellulose and single cell protein production:…”

“…The liquid medium containing the paper waste was used as the only source of carbon and energy in determining the factors affecting the production of the enzyme and the single cell protein, as it used conical flasks with a capacity of 250 milliliters and put in each of them 100 milliliters of the liquid medium and sterilized the decanters in the receptacle. And incubated in a rocker incubator at a speed of 120 shake / minute using various criteria including the pH, adjust the pH of the production medium (5,6,7,8,9,10) to determine the optimal pH for the production of the enzyme and single-cell protein for the bacterial isolation used. Different temperatures 20,25,30,35,40,45,50 C were selected to determine the optimum temperature for the production of the enzyme and protein.…”