Statistical optimization of medium components and growth conditions by response surface methodology to enhance lipase production by Aspergillus carneus

Kaushik, Rekha; Saran, Saurabh; Isar, Jasmine; Saxena, Rajendra Kumar

doi:10.1016/j.molcatb.2006.02.019

Cited by 170 publications

(97 citation statements)

References 14 publications

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“…Similar RSM using both nutritional and physiological factors have been reported by Kaushik et al [7] and Wang et al [13]. However, Sharma et al [14] used only three physical parameters i.e.…”

Section: Resultssupporting

confidence: 63%

“…Lipase production from Aspergillus carneus, Aspergillus terreus, Aspergillus niger have been reported by several investigators [3,6,7]. Previous investigations in our laboratory has shown Aspergillus carneus and Aspergillus terreus as potent lipase producers.…”

Section: Introductionmentioning

confidence: 59%

“…Previous investigations in our laboratory has shown Aspergillus carneus and Aspergillus terreus as potent lipase producers. We have reported certain results on production of these lipases [3,7,8]. In continuation of this, here we present some further studies carried out on process optimization of lipase from Aspergillus terreus by a statistical design method and its use in glyceride synthesis.…”

Section: Introductionmentioning

confidence: 88%

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Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

et al. 2010

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Aspergillus terreus produces lipase 7.01 IU/ml in 96 h after optimization by one variable at a time method. Using the significant factors i.e. corn oil (A), sodium nitrate (B), casein (C), agitation rate (D) and incubation period (E) RSM was carried out resulting in 19.65 IU/ml from the combination ?1(A), -1(B), -1(C), ?1(D) and 0(E). The interactions between sodium nitrate, casein and agitation with corn oil were most significant. Scale up of production from 250 ml shake flask to 30 l bioreactor resulted in increased productivity of 0.52 IU/ml/h as against 0.2 IU/ ml/h obtained in shake flasks. This lipase could carryout solvent free synthesis of partial glycerides of oleic acid with 96% efficiency in 12 h.

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Section: Resultssupporting

confidence: 63%

Section: Introductionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 88%

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Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

et al. 2010

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“…PB, in combination with RSM, has resulted in optimizing the medium and increasing the enzyme yield from a multitude of yeasts, fungi, and bacteria. Rhizopus delemar (Acıkel et al 2010), A. carneus (Kaushik et al 2006), Aspergillus awamori (Basheer et al 2011), Geotrichum sp. (Burkert et al 2004), C. cylindracea (Salihu et al 2011), Burkholderia sp.…”

Section: The Use Of Statistical Tools For Optimizing Lipase Productionmentioning

confidence: 99%

An insight into microbial lipases and their environmental facet

Kanmani¹,

Aravind²,

Kumaresan³

2014

Int. J. Environ. Sci. Technol.

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Lipases are serine hydrolases that catalyze the hydrolysis and synthesis of esters formed from glycerol and long-chain fatty acids, by acting at the oil-water interface. Lipases from microbial sources have received heightened attention for an array of industrial applications, and these enzymes have been well exploited in the environmental sector as well. In this article, we present an overview of microbial lipase, including the microorganisms from which it could be produced; the application of recombinant DNA technology tools to produce lipase with enhanced properties, the effective use of waste materials as substrates for lipase production; the usage of statistical tools to efficiently optimize the production medium; lipase purification strategies; and the immobilization of the enzyme on a variety of support materials. The next section of the article provides a gist of its application in diversified spheres and focusses exclusively on the environmentally relevant ones. Lipasecatalyzed esterification, transesterification, and interesterification reactions, an emerging area of green chemistry; lipase-mediated in vitro biopolymer synthesis and degradation; and the application of lipase for remediating fat and oil constituents in wastewater are dealt with in-depth. When its full potential is harnessed, the enzyme could play a pivotal role in environmental management.

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“…After optimization using RSM, 1.4-fold increase in lipase activity was observed with 27.25 U/ml lipase activity. Kaushik et al (2006) reported approximately 1.8-fold increase in the enzyme activity, resulting in 12.7 U/ml lipase activity. The optimum condition for lipase production was 34°C , oil concentration 3%, inoculum volume 7%, pH 7 and 60 h of incubation.…”

Section: Analysis Of Response Surfacementioning

confidence: 96%

Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes

Kumari

Mahapatra

Banerjee

2009

Braz. arch. biol. technol.

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Statistical optimization of medium components and growth conditions by response surface methodology to enhance lipase production by Aspergillus carneus

Cited by 170 publications

References 14 publications

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

Optimization of Lipase Production from Aspergillus terreus by Response Surface Methodology and Its Potential for Synthesis of Partial Glycerides Under Solvent Free Conditions

An insight into microbial lipases and their environmental facet

Statistical optimization of culture conditions by response surface methodology for synthesis of lipase with Enterobacter aerogenes

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