Statistical optimization of medium components for production of extracellular chitinase byBasidiobolus ranarum: a novel biocontrol agent against plant pathogenic fungi

Abstract

The influence of concentration of medium components such as colloidal chitin, lactose, malt extract, yeast extract, and peptone on the chitinase production from Basidiobolous ranarum at the flask level were studied by using statistical tool Central Composite Design (CCD) and analysed by Response Surface Methodology (RSM). The results revealed that colloidal chitin, malt extract and peptone had significant effect (P < 0.01) on the chitinase production at their individual levels. The polynomial equation of the model developed incorporates 3 linear, 3 quadratic and 5 interactive terms. Maximum chitinase production of 3.47 U ml(-1) was achieved with 1.5% colloidal chitin, 0.125% lactose, 0.025% malt extract and 0.075% peptone. After optimization, chitinase activity was increased by 7.71 fold. A second order polynomial equation was found to be useful for the development of efficient bioprocess for chitinase production. To screen the biotechnological potential of this enzyme, degradation of fungal mycelia by ammonium sulphate precipitate of the same was studied for several pathogenic fungi-in vitro which showed promising results particularly against Rhizoctonia solani and Fusarium solani. This study provides the first evidence showing the effectiveness of RSM for the development of a robust statistical model for the chitinase production by Basidiobolus and for its application in the biocontrol of phytopathogenic fungi.

Application of Statistically Based Experimental Designs to Optimize Cellulase Production and Identification of Gene

“…The biosynthesis of cellulases in  Trichoderma reesei  was very high in medium with carboxymethylcellulose as carbon source [12]. Similarly maximum amount of chitinase (3.47 U/mL) was produced by Basidiobolus ranarum with 1.5 % colloidal chitin, 0.125 % lactose, 0.025 % malt extract and 0.075 % peptone [25]. Li et al [26] reported maximum cellulase activity (0.26 U/mL) of a  Bacillus  sp.…”

Chitinolytic Microorganisms and Their Possible Application in Environmental Protection

“…melonis , Sclerotium rolfsii [114] Trichoderma harzianum Macrophomina phaseolina , Fusarium sp. R. solani , Aspergillus niger (NCIM 563), Aspergillus , Rhizopus , Mucor sp.[4, 70, 75] Trichoderma atroviride PTCC5220 Rhizoctonia solani [39] Trichothecium roseum Alternaria alternata , Fusarium moniliforme, Magnaporthe grisea [22] Basidiobolus ranarum Rhizoctonia solani , F. solani .[68] Bacillus sp. BG-11 Rhizopus arrhizus , Sclerotium rolfsii , R. solani, Phytophthora infestans , F. oxysporum , Phanerochaete chrysosporium [7] Bacillus cereus YQQ 308 Fusarium oxysporum , F. solani , P. ultimum [11] Bacillus pumilus SG2 Fusarium graminearum , R. solani , Magnaporthe grisea , Sclerotinia sclerotiorum , Trichoderma reesei , B. cinerea , Bipolaris sp.[28] Bacillus cereus IO8 Botrytis cinerea [36] Bacillus thuringiensis subsp.…”

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

“…[1] A large number of microorganisms are a preferred sources of chitinases, because they produce high amounts of these enzymes and it is easy to control their cultural conditions and to follow their harvesting procedures. [2] Chitinases, used in agriculture, are known to carry out functions as biological control agents against root-knot nematode eggs. [3] Meloidogyne incognita is a major plant-parasitic root-knot nematode species, which affects the final yields, production and quality of many annual and perennial crops.…”