Statistical Optimization of Medium Composition for Bacterial Cellulose Production by Gluconacetobacter hansenii UAC09 Using Coffee Cherry Husk Extract – an Agro-Industry Waste

Rani, M. Usha; Rastogi, N. K.; Appaiah, K. A. Anu

doi:10.4014/jmb.1012.12026

Cited by 66 publications

(32 citation statements)

References 20 publications

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“…Many agricultural and industrial wastes have been already investigated for the BNC production, including cashew tree residues 16 , dry olive mill residues 17 , konjac powder 18 , rice bark 19 , waste beer yeast 20 , oat hulls 21 , and coffee cherry husk 22 . However, there is still substantial interest to find out and utilize economical substrates that could promote the yield of BNC.…”

Section: Resultsmentioning

confidence: 99%

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Abol-Fotouh

Hassan

Shokry

et al. 2020

Sci Rep

180

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Bacterial nanocellulose (Bnc) has been drawing enormous attention because of its versatile properties. Herein, we shed light on the BNC production by a novel bacterial isolate (MD1) utilizing various agroindustrial wastes. Using 16S rRNA nucleotide sequences, the isolate was identified as Komagataeibacter saccharivorans MD1. For the first time, BNC synthesis by K. saccharivorans MD1 was investigated utilizing wastes of palm date, fig, and sugarcane molasses along with glucose on the Hestrin-Schramm (HS) medium as a control. After incubation for 168 h, the highest BNC yield was perceived on the molasses medium recording 3.9 g/L with an initial concentration of (v/v) 10%. The physicochemical characteristics of the BNC sheets were inspected adopting field-emission scanning electron microscope (FESEM), atomic force microscopy (AFM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) analysis. The FESEM characterization revealed no impact of the wastes on either fiber diameter or the branching scheme, whereas the AFM depicted a BNC film with minimal roughness was generated using date wastes. Furthermore, a high crystallinity index was estimated by XRD up to 94% for the date wastes-derived BNC, while the FTIR analyses exhibited very similar profiles for all BNC films. Additionally, mechanical characteristics and water holding capacity of the produced Bncs were studied. Our findings substantiated that expensive substrates could be exchanged by agro-industrial wastes for Bnc production conserving its remarkable physical and microstructural properties. In the last decades, bacterial nanocellulose (BNC) has earned increasing global interest because of its remarkable physical and chemical properties, including green processing, low production costs, elevated mechanical properties, hydrophilicity, excellent biocompatibility, and biodegradability 1,2. Certain gram-negative non-pathogenic bacterial genera like Rhizobium, Xanthococcus, Pseudomonas, Azotobacter, Aerobacter, and Alcaligenes were reported to produce nanocellulose extracellularly, but the most common BNC-producing strains belong to the genus Komagataeibacter (formerly Acetobacter or commonly acetic acid bacteria) 1. Bacteria produce the BNC through a process of dual coupled steps: polymerization and crystallization. In the bacterial cytoplasm, glucose residues polymerize to β-1,4 glucan linear chains where they are extracellularly secreted. The developed chains are crystallized to microfibrils, then certain numbers of microfibrils consolidate to materialize highly pure 3D porous network of entangled nanoribbons of 20-60 nm in width 3 .

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Section: Resultsmentioning

confidence: 99%

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Abol-Fotouh

Hassan

Shokry

et al. 2020

Sci Rep

180

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“…As the substrates, especially the carbon source, can determine the costs of microbial fermentation processes, and particularly for BC production the culture media can be responsible for up to 65 % of the total process cost, several studies have been devoted to find new low-cost carbon sources, as glycerol remaining from biodiesel production and grape bagasse, a residue of wine production, coffee cherry husk extract, and corn steep liquor (Moosavi-Nasab and Yousefi 2011 ;Vazquez et al 2013;Castro et al 2013;Rani et al 2011;Carreira et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media

Jozala

Pértile

Santos

et al. 2014

Appl Microbiol Biotechnol

168

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Bacterial cellulose (BC) is used in different fields as a biological material due to its unique properties. Despite there being many BC applications, there still remain many problems associated with bioprocess technology, such as increasing productivity and decreasing production cost. New technologies that use waste from the food industry as raw materials for culture media promote economic advantages because they reduce environmental pollution and stimulate new research for science sustainability. For this reason, BC production requires optimized conditions to increase its application. The main objective of this study was to evaluate BC production by Gluconacetobacter xylinus using industry waste, namely, rotten fruits and milk whey, as culture media. Furthermore, the structure of BC produced at different conditions was also determined. The culture media employed in this study were composed of rotten fruit collected from the disposal of free markets, milk whey from a local industrial disposal, and their combination, and Hestrin and Schramm media was used as standard culture media. Although all culture media studied produced BC, the highest BC yield-60 mg/mL-was achieved with the rotten fruit culture. Thus, the results showed that rotten fruit can be used for BC production. This culture media can be considered as a profitable alternative to generate high-value products. In addition, it combines environmental concern with sustainable processes that can promote also the reduction of production cost.

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“…RSM is then used to investigate the optimum region of significant factors [21]. It has many benefits including lean experiment design space, reliability, and assisting in understanding the interactions among the factors of various concentrations and, especially, successful optimization of medium compositions and fermentation process parameters [28,29].…”

mentioning

confidence: 99%

Statistical Optimization for Monacolin K and Yellow Pigment Production and Citrinin Reduction by Monascus purpureus in Solid-State Fermentation

Jirasatid¹,

Nopharatana²,

Kitsubun³

et al. 2013

J. Microbiol. Biotechnol.

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Monacolin K and yellow pigment, produced by Monascus sp., have each been proven to be beneficial compounds as antihypercholesterolemic and anti-inflammation agents, respectively. However, citrinin, a human toxic substance, was also synthesized in this fungus. In this research, solidstate fermentation of M. purpureus TISTR 3541 was optimized by statistical methodology to obtain a high production of monacolin K and yellow pigment along with a low level of citrinin. Fractional factorial design was applied in this study to identify the significant factors. Among the 13 variables, five parameters (i.e., glycerol, methionine, sodium nitrate, cultivation time, and temperature) influencing monacolin K, yellow pigment, and citrinin production were identified. A central composite design was further employed to investigate the optimum level of these five factors. The maximum production of monacolin K and yellow pigment of 5,900 mg/kg and 1,700 units/g, respectively, and the minimum citrinin concentration of 0.26 mg/kg were achieved in the medium containing 2% glycerol, 0.14% methionine, and 0.01% sodium nitrate at 25°C for 16 days of cultivation. The yields of monacolin K and yellow pigment were about 3 and 1.5 times higher than the basal medium, respectively, whereas citrinin was dramatically reduced by 36 times.

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Statistical Optimization of Medium Composition for Bacterial Cellulose Production by Gluconacetobacter hansenii UAC09 Using Coffee Cherry Husk Extract – an Agro-Industry Waste

Cited by 66 publications

References 20 publications

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Bacterial nanocellulose from agro-industrial wastes: low-cost and enhanced production by Komagataeibacter saccharivorans MD1

Bacterial cellulose production by Gluconacetobacter xylinus by employing alternative culture media

Statistical Optimization for Monacolin K and Yellow Pigment Production and Citrinin Reduction by Monascus purpureus in Solid-State Fermentation

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