Statistical optimization of production conditions of β-glucosidase from Bacillus stratosphericus strain SG9

Abstract

The present study illustrates the optimization and characterization of β-glucosidase from a bacterial isolate, strain SG9. Sixty-eight different variables were first screened by one factor at a time method. The screened variable optimization was then performed by Plackett-Burman design followed by Box-Behnken response surface methodology. Thirty-one variables were screened, of which five variables were found to be significant. Box-Behnken design was then performed using the most significant variables, viz., esculin, KHPO and MgSO. The maximum enzyme activity was observed with an optimal medium composition of esculin (1.9 g/L), KHPO (0. 5 g/L) and MgSO (0.3 g/L) with a predicted value of 3392.01 IU. The maximum β-glucosidase production achieved was 3340 IU. The bacterial strain was identified by 16S rRNA gene sequence and biochemical characterization. The strain was identified as Bacillus stratosphericus and is a first report of its kind.

Isolation of the Thermostable β-Glucosidase-Secreting Strain Bacillus altitudinis JYY-02 and Its Application in the Production of Gardenia Blue

“…β-Glucosidases are widely applied in the food, animal feed, pharmacy, and textile industries. Several Bacillus species, such as B. subtilis ( 17 ) and B. stratosphericus ( 18 ), have been found to secrete β-glucosidases, but the enzyme yield still needs to be improved. The fungus Trichoderma reesei was also reported to produce β-glucosidase but in very small amounts ( 19 ).…”

A Thermotolerant Yeast Cyberlindnera rhodanensis DK Isolated from Laphet-so Capable of Extracellular Thermostable β-Glucosidase Production

“…When In this investigation, PBD and RSM were employed to identify the critical factors affecting β-glucosidase production by C. rhodanensis DK. Numerous research reports have documented the application of these methods for medium optimization with various microorganims, including Zobellella denitrificans [74], A. versicolor [75] and B. stratosphericus [76]. Based on the findings from PBD, it was evident that among the six component factors considered, yeast extract, peptone and xylose/xylan had a statistically significant effect on enzyme production.…”

Oil Palm Empty Fruit Bunch (OPEFB) Handsheet Production from Optimized Biodelignification of Rhynchophorus Ferrugineus Microbiome’s Enzymes

“…Moreover, the result of the degree of freedom (DF) is 9 which is equivalent to the independent observation number. The p value can be taken as factor for significant verification of each coefficient which explains the interaction between the strength of each parameter and mutual interaction pattern between variables [30]. Several criteria were used to check goodness of fit of the model.…”

“…The determination coefficient (R 2 ) value 0.9372 indicates that the model was able to explain 93.72% of the variability in the response. In addition, the value of R 2 also is presented a correlation between the predicted and the experimental values [30], [31]. Also the adjusted determination coefficient (adj.…”