Statistical screenings of medium components for the production of chitinase by the marine isolate Pantoea dispersa

Gohel, Vipul; Chaudhary, Tejas; Vyas, Pranav; Chhatpar, H. S.

doi:10.1016/j.bej.2005.09.002

Cited by 48 publications

(21 citation statements)

References 33 publications

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“…Pantoea is a member of the Enterobacteriaceae, but is found in a wide range of habitats. Members of the genus have been linked to EPS degradation in cooling tower biofilms (Ceyhan and Ozdemir 2008), and a marine isolate of the genus has been found to produce chitinase and glucosidases capable of degradation of complex macromolecules (Gohel et al 2006). Given that Bacteroidetes have been found associated with diatoms and estuarine biofilms (Sapp et al 2007;Teeling et al 2012), it is notable that we did not identify them in the most heavily 13 C-labeled fractions.…”

Section: Discussionmentioning

confidence: 91%

Microphytobenthic extracellular polymeric substances (EPS) in intertidal sediments fuel both generalist and specialist EPS‐degrading bacteria

Taylor

McKew

Kuhl

et al. 2013

Limnology & Oceanography

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Microphytobenthic biofilms contain high concentrations of carbohydrate-rich extracellular polymeric substances (EPS) that are important in sediment carbon cycling. Field measurements at two locations in the Colne Estuary, U.K., showed that a significant curvilinear relationship explained 50% of the variability in chlorophyll a and EPS content. Estimates of EPS production, based on field data and published rates of production by diatoms, revealed that EPS turnover of 52% to 369% over the tidal cycle was required to account for field standing stocks. We investigated EPS degradation in sediment slurries using purified 13 C-EPS produced by the diatom Nitzschia tubicola. Although EPS constituted only 5% of the sediment dissolved organic carbon (DOC) pool, 100% of the added EPS was utilized within 30 h, before decreases in other sediment-carbohydrate fractions and DOC concentrations. A general 13 C enrichment of phospholipid fatty acids (PLFAs), representative of Gram-positive and Gram-negative bacteria, occurred within 6 h, with the PLFAs a15:0, i15:0, and 18:1v7c being highly enriched. The diatom PLFA 20:5v3 had relatively low but significant 13 C enrichment. Stable isotope probing of 16S ribosomal ribonucleic acid (RNA-SIP) at 30 h revealed 13 C-enriched sequences from the diatom genus Navicula; further evidence that diatoms assimilated the EPS, or EPS-breakdown products, from other diatom taxa. RNA-SIP also demonstrated a diverse range of highly 13 C-enriched bacterial taxa, including a distinct subset (Alphaproteobacteria and Gammaproteobacteria) found only in the heavily labeled microbial assemblages. Thus, cycling of diatom EPS is rapid, and involves a wide range of microbial taxa, including some apparent specialists.

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Section: Discussionmentioning

confidence: 91%

Microphytobenthic extracellular polymeric substances (EPS) in intertidal sediments fuel both generalist and specialist EPS‐degrading bacteria

Taylor

McKew

Kuhl

et al. 2013

Limnology & Oceanography

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“…[38] The chitinase production was enhanced 4.21-fold by using a statistical medium optimization method in P. dispersa and 4.33-fold where purified chitin compound was used. [36,43] In this study, the 2.87-fold increase in chitinase activity indicated that the PBD and CCD RSM were useful tools for screening factors and optimizing medium components for chitinase production by P. fluorescens strain HN1205. The direct utilization of chitincontaining marine crustacean waste and the bioconversion of shell fish chitin waste has been recently investigated for the production of chitinases.…”

Section: Selection Of Important Medium Components Using Plackettàburmmentioning

confidence: 86%

“…[26,33,34] Shrimp shell waste containing chitin source was reported to be an important factor for chitinase production. [35] Gohel et al [36] has reported CaCl 2 as an influential media constituent for chitinase production by Pantoea dispersa. X 2 (KH 2 PO 4 ), X 3 ((NH 4 ) 2 SO 4 ), X 4 (MgSO 4 ¢7H 2 O) and X 5 (sucrose) are below 90% and therefore considered to be insignificant variables.…”

Section: Selection Of Important Medium Components Using Plackettàburmmentioning

confidence: 99%

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

Lee

Kim

2015

Biotechnology & Biotechnological Equipment

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In the present research, statistical PlackettÀBurman design (PBD) and central composite design (CCD) were used to optimize the medium's components in order to enhance the chitinase activity of Pseudomonas fluorescens strain HN1205. Among the seven nutritional elements that were studied, crab shell powder, CaCl 2 ¢2H 2 O and yeast extract were proved using PBD to have significant effect on chitinase activity. An optimal medium was obtained by applying a three-factor central composite design, which consisted of crab shell powder (1.0 g/L), CaCl 2 (0.5 g/L) and yeast extract (0.5 g/L), with the highest chitinase activity of 1.03 U/mL. This value was 2.87-fold higher than the activity obtained in the lowest productive medium in the design matrix. The chitinase produced by the HN1205 strain was partially purified with 80% ammonium sulphate and anion-exchange chromatography and assessed for inhibition of hatching of nematode eggs. The partially purified chitinase significantly reduced the hatch of Meloidogyne incognita eggs (8.1%), as compared to the effect of 10 mmol/L Tris-HCl buffer (pH 8.0) (49.4%) and boiled chitinase (54.7%). This study demonstrated the role of chitinolytic enzymes produced by P. fluorescens strain HN1205. The obtained optimal activity proved that the enzymes could be potential biological control agents.

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“…One of the optimization methodologies that has been used in industrial processes is the response surface methodology (RSM); it combines mathematical and statistical techniques to build empirical models (Hanrahan and Lu, 2006). This methodology is advantageous because it allows identifying the effect of factors that generate a basis for additional experiments and setting values to factors that improve performance; this leads to savings in time, materials, and labor (Gohel et al, 2006). Therefore, the aim of this study was to optimize the mass production of Metarhizium anisopliae in different substrates.…”

Section: Experimental Designmentioning

confidence: 99%

Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates

Barra-Bucarei

Vergara

Cortés

2016

Chilean J. Agric. Res.

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One of the best alternatives to reduce the amount of chemical insecticides released into the environment is biological agents. Metarhizium anisopliae (Metschn.) Sorokin 1883 (Hypocreales: Clavicipitaceae) is an entomopathogenic fungus with great potential as a biological pesticide to biologically control pests. However, the relatively high cost of the substrate needed for its mass production system increases product price and discourages its use. The objective of this study was to optimize the mass production conditions of M. anisopliae for use as a biological control agent using two solid substrates, new parboiled rice (NPR) and recycled parboiled rice (RPR). Conidial production was optimized by the response surface methodology (RSM). The effects of the temperature, time, and molasses variables and the interactions between them (conidia g -1 ) were determined. For the NPR substrate, it was determined that the significant variables were time and temperature, and the interactions were temperature × molasses and temperature × time. For the RPR substrate, the significant variables were temperature and time, and the interactions were time × molasses and temperature × time. Both substrates obtained the highest industrial yields at 25 °C for a period of 20 d. Given that the percentage of molasses was not critical for yields, it is recommended that it be set at 5% to reduce costs. Finally, it was possible to use the RPR substrate from the M. anisopliae produ9ction itself as an alternative to solid substrate; mean industrial performance (conidia g -1 ) was higher than values obtained with NPR and at a lower cost.

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Statistical screenings of medium components for the production of chitinase by the marine isolate Pantoea dispersa

Cited by 48 publications

References 33 publications

Microphytobenthic extracellular polymeric substances (EPS) in intertidal sediments fuel both generalist and specialist EPS‐degrading bacteria

Microphytobenthic extracellular polymeric substances (EPS) in intertidal sediments fuel both generalist and specialist EPS‐degrading bacteria

Statistical optimization of medium components for chitinase production byPseudomonas fluorescensstrain HN1205: role of chitinase on egg hatching inhibition of root-knot nematode

Conditions to optimize mass production of Metarhizium anisopliae (Metschn.) Sorokin 1883 in different substrates

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