Statistical thermodynamics of molecules with multiple adsorption states: Application to protein adsorption

Quiroga, Evelina; Ramírez-Pastor, A. J.

doi:10.1016/j.cplett.2012.11.019

Cited by 16 publications

(29 citation statements)

References 50 publications

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“…The successful fit of our data by the Langmuir and modified Langmuir models suggests that these models are adequate for describing the behavior of the system. Although other approaches such as statistical thermodynamics may provide a slightly more accurate fit to the data, they would not significantly impact the essence of our results.…”

Section: Discussionmentioning

confidence: 99%

Utilizing Avidity To Improve Antifreeze Protein Activity: A Type III Antifreeze Protein Trimer Exhibits Increased Thermal Hysteresis Activity

Can

Holland

2013

Biochemistry

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Antifreeze proteins (AFPs) are ice growth inhibitors that allow the survival of several species living at temperatures colder than the freezing point of their bodily fluids. AFP activity is commonly defined in terms of thermal hysteresis, which is the difference observed for the solution freezing and melting temperatures. Increasing the thermal hysteresis activity of these proteins, particularly at low concentrations, is of great interest because of their wide range of potential applications. In this study, we have designed and expressed one-, two-, and three-domain antifreeze proteins to improve thermal hysteresis activity through increased binding avidity. The three-domain type III AFP yielded significantly greater activity than the one- and two-domain proteins, reaching a thermal hysteresis of >1.6 °C at a concentration of <1 mM. To elucidate the basis of this increase, the data were fit to a multidomain protein adsorption model based on the classical Langmuir isotherm. Fits of the data to the modified isotherms yield values for the equilibrium binding constants for the adsorption of AFP to ice and indicate that protein surface coverage is proportional to thermal hysteresis activity.

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Section: Discussionmentioning

confidence: 99%

Utilizing Avidity To Improve Antifreeze Protein Activity: A Type III Antifreeze Protein Trimer Exhibits Increased Thermal Hysteresis Activity

Can

Holland

2013

Biochemistry

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“…Equilibrium parameters of adsorption. To mathematically process the experimental data, the two-parameter models by Langmuir, Freundlich, Temkin and the three-parameter model by Langmuir-Freundlich were used [26][27][28] (Table 3). Error functions.…”

Section: Methodsmentioning

confidence: 99%

Effect of the membrane/protein interaction nature on adsorptive properties of ultrafiltration membranes based on aromatic poly- and copolyamides

Смирнова¹,

Nebukina

2018

sorpchrom

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Membranes are a science intensive product of interindustry use, without which a breakthrough development of basic and high-technology sectors of economy, development of science as well as effective solution of important goals of the social sphere and problems of environment protections are impossible. Active development of medicine, pharmaceutical industry and biotechnology in the recent years contributed to the growth of scientific and commercial interest in ultrafiltration. The most important characteristic of ultrafiltration membranes is selectivity. According to existing views the basis of the separation mechanism implemented in ultrafiltration is size selectivity. However, such phenomena as concentration polarization and adsorption play a very important role in separation. Investigation of these phenomena is of significant practical interest, because its results in many ways determine the choice of the membrane media, conditions of its recovery, the mode and conditions of filtration. Adsorption of proteins on the surface of porous membranes is a rather complicated process due to the individual mechanism implemented in each specific case. Therefore, the issue of the contribution of various types of interactions into the process of adsorption still remains controversial. To determine the nature of membrane/protein interaction and clarify the factors of its control, the present work involved investigation of sorption of several proteins on a number of synthesized porous membranes based on aromatic polyamides noted for the presence, nature and concentration of ionogenic groups. The investigations were carried out using bovine serum albumin, lysozyme of hen's eggs, myoglobin and bacitracin. Adsorption of proteins by the membranes was investigated in the static mode. The protein concentration was determined using the SF-2000 spectrophotometer (experimental-design bureau Spektr) by optical density at the wave length λ=278 nm. To mathematically process the experimental data, the two-parameter models by Langmuir, Freundlich, Temkin and the three-parameter model by Langmuir-Freundlich were used. To assess the compliance degree of the experimental data to the selected mathematical models, the values of the coefficient of determination (R2) and sum squares errors (SSE) were used. It has been shown that in the case of the presence of charge in protein macromolecules and the membrane surface the role of electrostatic forces is dominant in the protein adsorption mechanism, however, the contribution of non-electrostatic interactions in the investigated membrane/protein systems is significant.

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“…Polymer adsorption on a substrate has received considerable attention, both because of its intrinsic merit as an interesting problem in statistical mechanics [4] and because of its technological importance in the stabilization of colloidal dispersions used in paints, pharmaceuticals and foodstuffs [5]. Another model of this genre for molecules with multiple adsorption states can be studied using a lattice model [6]. In this kind of model, the statistical thermodynamic functions are used to describe the adsorption of antifreeze proteins onto an ice crystal.…”

Section: Introductionmentioning

confidence: 99%

Entropy of dimers chains placed on a one-dimensional lattice with q-states

Nascimento¹,

Neto²,

Salmon³

et al. 2015

Physica A: Statistical Mechanics and its Applications

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Statistical thermodynamics of molecules with multiple adsorption states: Application to protein adsorption

Cited by 16 publications

References 50 publications

Utilizing Avidity To Improve Antifreeze Protein Activity: A Type III Antifreeze Protein Trimer Exhibits Increased Thermal Hysteresis Activity

Utilizing Avidity To Improve Antifreeze Protein Activity: A Type III Antifreeze Protein Trimer Exhibits Increased Thermal Hysteresis Activity

Effect of the membrane/protein interaction nature on adsorptive properties of ultrafiltration membranes based on aromatic poly- and copolyamides

Entropy of dimers chains placed on a one-dimensional lattice with q-states

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