Statistically designed optimisation of enzyme catalysed starch removal from potato pulp

Thomassen, Lise Vestergaard; Meyer, Anne S.

doi:10.1016/j.enzmictec.2009.11.003

Cited by 16 publications

(3 citation statements)

References 15 publications

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“…Separations were performed using a CarboPacTM PA20 (3 mm×150 mm) analytical column (Dionex Corp.) according to Thomassen and Meyer (2010). The quantification was carried out using the external monosaccharide standards: L-arabinose, L-rhamnose, L-fucose, D-galactose, D-glucose, D-mannose, D-xylose and D-glucuronic acid.…”

Section: Methodsmentioning

confidence: 99%

Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.

2011

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Fucose-containing sulfated polysaccharides can be extracted from the brown seaweed, Sargassum sp. It has been reported that fucose-rich sulfated polysaccharides from brown seaweeds exert different beneficial biological activities including anti-inflammatory, anticoagulant, and anti-viral effects. Classical extraction of fucose-containing sulfated polysaccharides from brown seaweed species typically involves extended, multiple-step, hot acid, or CaCl 2 treatments, each step lasting several hours. In this work, we systematically examined the influence of acid concentration (HCl), time, and temperature on the yield of fucosecontaining sulfated polysaccharides (FCSPs) in statistically designed two-step and single-step multifactorial extraction experiments. All extraction factors had significant effects on the fucose-containing sulfated polysaccharides yield, with the temperature and time exerting positive effects, and the acid concentration having a negative effect. The model defined an optimized single-step FCSPs extraction procedure for Sargassum sp. (a brown seaweed). A maximal fucosecontaining sulfated polysaccharides yield of ∼7% of the Sargassum sp. dry matter was achieved by the optimal extraction procedure of: 0.03 M HCl, 90°C, 4 h. HPAEC-PAD analysis confirmed that fucose, galactose, and glucuronic acid were the major constituents of the polysaccharides obtained by the optimized method. Lower polysaccharide yield, but relatively higher fucose content was obtained with shorter extraction time. The data also revealed that classical multi-step extraction with acid ≥0.2 M HCl at elevated temperature and extended time had a detrimental effect on the FCSPs yield as this treatment apparently disrupted the structural integrity of the polymer and evidently caused degradation of the carbohydrate chains built up of fucose residues.

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Section: Methodsmentioning

confidence: 99%

Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.

2011

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“…The insoluble pulp was washed with milli-Q ® water. For de-starching, the pulp was diluted in deionized water to 8% (w/w) dry matter and heated to 70 °C before the addition of 0.2% (v/w dry matter) Termamyl ® SC (Novozymes A/S, Bagsvaerd, Denmark) and the pulp suspension was then allowed to react for 65 min (Thomassen and Meyer, 2010). The pulp was washed with milli-Q ® water and filtered using a 12-inch Porcelain Büchner Funnel to remove the solubilized starch sugars resulting in destarched sweet potato pulp (Figure 1).…”

Section: Methodsmentioning

confidence: 99%

Discovery of novel secretome CAZymes from Penicillium sclerotigenum by bioinformatics and explorative proteomics analyses during sweet potato pectin digestion

Barrett

Zhao

Hao

et al. 2022

Front. Bioeng. Biotechnol.

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Novel selective enzymatic refining of sweet potato processing residues requires judicious enzyme selection and enzyme discovery. We prepared a pectinaceous cell wall polysaccharide fraction from sweet potato using an enzymatic a treatment to preserve the natural linkages and substitutions. Polysaccharide composition and linkage analysis data confirmed the pectinaceous polysaccharide fraction to be a rhamnogalacturonan I-rich fraction with a high content of arabinogalactan Type I. We hypothesized that the postharvest tuber pathogenic fungus Penicillium sclerotigenum would harbor novel enzymes targeting selective sweet potato pectin modification. As part of the study, we also report the first genome sequence of P. sclerotigenum. We incubated the sweet potato pectinaceous fraction with P. sclerotigenum. Using proteomics accompanied by CUPP-bioinformatics analysis, we observed induced expression of 23 pectin-associated degradative enzymes. We also identified six abundantly secreted, induced proteins that do not correspond to known CAZymes, but which we suggest as novel enzymes involved in pectin degradation. For validation, the predicted CUPP grouping of putative CAZymes and the exo-proteome data obtained for P. sclerotigenum during growth on sweet potato pectin were compared with proteomics and transcriptomics data reported previously for pectin-associated CAZymes from Aspergillus niger strain NRRL3. The data infer that P. sclerotigenum has the capacity to express several novel enzymes that may provide novel opportunities for sweet potato pectin modification and valorization of sweet potato starch processing residues. In addition, the methodological approach employed represents an integrative systematic strategy for enzyme discovery.

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“…The separation was made using a CarboPac TM PA20 (3 mm × 150 mm) analytical column based on a method of [37] with the following modifications: a two-eluent system comprising deionized water and a 0.5 M NaOH aqueous solution was used. Before injection of each sample (10 l) the column was reequilibrated with 15 mM NaOH for 10 min.…”

Section: Hpaec Analysismentioning

confidence: 99%

Tailored enzymatic production of oligosaccharides from sugar beet pectin and evidence of differential effects of a single DP chain length difference on human faecal microbiota composition after in vitro fermentation

Holck

Hjernø

Lorentzen

et al. 2011

Process Biochemistry

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Statistically designed optimisation of enzyme catalysed starch removal from potato pulp

Cited by 16 publications

References 15 publications

Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.

Designed optimization of a single-step extraction of fucose-containing sulfated polysaccharides from Sargassum sp.

Discovery of novel secretome CAZymes from Penicillium sclerotigenum by bioinformatics and explorative proteomics analyses during sweet potato pectin digestion

Tailored enzymatic production of oligosaccharides from sugar beet pectin and evidence of differential effects of a single DP chain length difference on human faecal microbiota composition after in vitro fermentation

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